A Detailed Model for High-Frequency Impedance Characterization of Ovarian Cancer Epithelial Cell Layer Using ECIS Electrodes

2009 ◽  
Vol 56 (2) ◽  
pp. 485-492 ◽  
Author(s):  
A.R.A. Rahman ◽  
Chun-Min Lo ◽  
S. Bhansali
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Cell ◽  
High Frequency ◽  
Cell Layer ◽  
Detailed Model ◽  
Epithelial Cell Layer ◽  
Impedance Characterization

1453: Lonidamine Reduces Prostate Weight in Rodents and is Accompanied by Apoptosis and Disruption of the Epithelial Cell Layer

2006 ◽  
Vol 175 (4S) ◽  
pp. 469-469
Author(s):  
W. Steve Ammons ◽  
Tatyana Lukoyanova ◽  
Amber Durnal ◽  
Geoffrey Del Rosario ◽  
Alan Colowick
Keyword(s):  
Epithelial Cell ◽  
Cell Layer ◽  
Prostate Weight ◽  
Epithelial Cell Layer

scholarly journals Infection with Rhinovirus Facilitates Allergen Penetration Across a Respiratory Epithelial Cell Layer

2015 ◽  
Vol 166 (4) ◽  
pp. 291-296 ◽  
Author(s):  
Katharina Gangl ◽  
Eva E. Waltl ◽  
Helga Vetr ◽  
Clarissa R. Cabauatan ◽  
Katarzyna Niespodziana ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Layer ◽  
Respiratory Epithelial Cell ◽  
Epithelial Cell Layer ◽  
Respiratory Epithelial

scholarly journals Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Ang Su ◽  
Jie Tong ◽  
Yuguang Fu ◽  
Sandy Müller ◽  
Yenehiwot Berhanu Weldearegay ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Layer ◽  
Airway Epithelial Cells ◽  
Infection Model ◽  
Target Cells ◽  
Respiratory Disorder ◽  
Airway Epithelial ◽  
Epithelial Cell Layer ◽  
Well Differentiated

AbstractPasteurella (P.) multocida is a zoonotic pathogen, which is able to cause respiratory disorder in different hosts. In cattle, P. multocida is an important microorganism involved in the bovine respiratory disease complex (BRDC) with a huge economic impact. We applied air–liquid interface (ALI) cultures of well-differentiated bovine airway epithelial cells to analyze the interaction of P. multocida with its host target cells. The bacterial pathogen grew readily on the ALI cultures. Infection resulted in a substantial loss of ciliated cells. Nevertheless, the epithelial cell layer maintained its barrier function as indicated by the transepithelial electrical resistance and the inability of dextran to get from the apical to the basolateral compartment via the paracellular route. Analysis by confocal immunofluorescence microscopy confirmed the intactness of the epithelial cell layer though it was not as thick as the uninfected control cells. Finally, we chose the bacterial neuraminidase to show that our infection model is a sustainable tool to analyze virulence factors of P. multocida. Furthermore, we provide an explanation, why this microorganism usually is a commensal and becomes pathogenic only in combination with other factors such as co-infecting microorganisms.

scholarly journals The Characteristics and Expression Profile of Transferrin in the Accessory Nidamental Gland of the Bigfin Reef Squid during Bacteria Transmission

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hau-Wen Li ◽  
Chih Chen ◽  
Wei-Lun Kuo ◽  
Chien-Ju Lin ◽  
Ching-Fong Chang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Outer Layer ◽  
Cell Layer ◽  
Reproductive Organ ◽  
Iron Sequestration ◽  
Nidamental Gland ◽  
Epithelial Cell Layer ◽  
Bacterial Transmission

AbstractThe accessory nidamental gland (ANG) is a female reproductive organ found in most squid and cuttlefish that contains a consortium of bacteria. These symbiotic bacteria are transmitted from the marine environment and selected by the host through an unknown mechanism. In animals, a common antimicrobial mechanism of innate immunity is iron sequestration, which is based on the development of transferrin (TF)-like proteins. To understand this mechanism of host-microbe interaction, we attempted to characterize the role of transferrin in bigfin reef squid (Sepioteuthis lessoniana) during bacterial transmission. qPCR analysis showed that Tf was exclusively expressed in the outer layer of ANG,and this was confirmed by in situ hybridization, which showed that Tf was localized in the outer epithelial cell layer of the ANG. Western blot analysis indicated that TF is a soluble glycoprotein. Immunohistochemical staining also showed that TF is localized in the outer epithelial cell layer of the ANG and that it is mainly expressed in the outer layer during ANG growth. These results suggest that robust Tf mRNA and TF protein expression in the outer layer of the ANG plays an important role in microbe selection by the host during bacterial transmission.

The epithelial barrier-protecting properties of a soy hydrolysate

2018 ◽  
Vol 9 (8) ◽  
pp. 4164-4172 ◽  
Author(s):  
Mensiena B. G. Kiewiet ◽  
Martín I. González Rodríguez ◽  
Renske Dekkers ◽  
Marjan Gros ◽  
Laurien H. Ulfman ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Layer ◽  
Epithelial Barrier ◽  
Specific Manner ◽  
Epithelial Cell Layer

A soy hydrolysate protects the epithelial cell layer from an increase in permeability in a barrier disruptor specific manner.

scholarly journals Change in the Electrical Impedance Caused by Cornification of the Epithelial Cell Layer of the Vaginal Mucosa in the Rat

1987 ◽  
Vol 36 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Masao KOTO ◽  
Masao MIWA ◽  
Ko-ichiro TSUJI ◽  
Michio OKAMOTO ◽  
Jiro ADACHI
Keyword(s):  
Epithelial Cell ◽  
Electrical Impedance ◽  
Cell Layer ◽  
Vaginal Mucosa ◽  
Epithelial Cell Layer

Modelling wound closure in an epithelial cell sheet using the cellular Potts model

2015 ◽  
Vol 7 (10) ◽  
pp. 1253-1264 ◽  
Author(s):  
Adrian R. Noppe ◽  
Anthony P. Roberts ◽  
Alpha S. Yap ◽  
Guillermo A. Gomez ◽  
Zoltan Neufeld
Keyword(s):  
Epithelial Cell ◽  
Potts Model ◽  
Wound Closure ◽  
Cell Layer ◽  
Cell Sheet ◽  
Two Dimensional ◽  
Cellular Potts Model ◽  
Epithelial Cell Layer

We use a two-dimensional cellular Potts model to represent the behavior of an epithelial cell layer and describe its dynamics in response to a microscopic wound.

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