Characterization of immobilized antibodies on silica surfaces

1988 ◽  
Vol 35 (6) ◽  
pp. 466-471 ◽  
Author(s):  
Jinn-Nan Lin ◽  
J. Herron ◽  
J.D. Andrade ◽  
M. Brizgys
Keyword(s):  
Silica Surfaces ◽  
Immobilized Antibodies

Chromatographic characterization of silica surfaces

1981 ◽  
Vol 218 ◽  
pp. 395-407 ◽  
Author(s):  
Heinz Engelhardt ◽  
Hartmut Müller
Keyword(s):  
Silica Surfaces ◽  
Chromatographic Characterization

scholarly journals Affinity purification and characterization of protease-susceptible antigen I of Streptococcus mutans

1980 ◽  
Vol 29 (3) ◽  
pp. 999-1006 ◽  
Author(s):  
M W Russell ◽  
E D Zanders ◽  
L A Bergmeier ◽  
T Lehner
Keyword(s):  
Streptococcus Mutans ◽  
Affinity Purification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Immobilized Antibodies ◽  
Homogeneous Product ◽  
Monospecific Antisera ◽  
Culture Supernatants

An antigenic component (antigen I) of the cell surface of Streptococcus mutans has been purified from culture supernatants and shown to be immunologically identical to the protease-susceptible moiety of antigen I/II. Ion-exchange and gel filtration chromatography failed to yield a physicochemically homogeneous product. Immunoasbsorbent chromatography on single and tandem columns containing immobilized antibodies to antigens I/II and II yielded identical products which were homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and which when injected into rabbits induced monospecific antisera to antigen I. This antigen consisted of approximately 70% protein. Its molecular weight was estimated as 150,000, and the isoelectric point was estimated to be 5.1. Immunofluorescence microscopy using monospecific antiserum to antigen I showed that a similar antigen was present on cells of S. mutans serotypes a, c, d, e, f, and g, but not b.

Synthesis and characterization of immobilized dendritic silica surfaces

2002 ◽  
Vol 5 (1) ◽  
pp. 67-77 ◽  
Author(s):  
George R. Newkome ◽  
Kyung Soo Yoo ◽  
Charles N. Moorefield
Keyword(s):  
Synthesis And Characterization ◽  
Silica Surfaces

Covalent Grafting of Organoalkoxysilanes on Silica Surfaces in Water-Rich Medium as Evidenced by 29Si NMR

2007 ◽  
Vol 1007 ◽  
Author(s):  
Christian Bonhomme ◽  
Sophie De Monredon ◽  
François Ribot ◽  
Florence Babonneau
Keyword(s):  
Silica Surface ◽  
Point Of View ◽  
Experimental Investigations ◽  
29Si Nmr ◽  
Rich Medium ◽  
Spectroscopy Analysis ◽  
Huge Amount ◽  
Silica Surfaces ◽  
Covalent Grafting

ABSTRACTDespite the huge amount of experimental investigations dedicated to the grafting of organoalkoxysilanes on silica, the characterization of covalent linkages remains a difficult task from a spectroscopic point of view. In most cases, the formation of a covalent link is taken for granted and not discussed. 29Si NMR, IR or UV spectroscopies are commonly used to study the modification of silica surface. Herein we report an unambiguous method to evidence the formation of covalent links. It is based on NMR spectroscopy analysis coupled with a rigorous synthetic grafting protocol.

Characterization of silica surfaces by free radical spin labels

1977 ◽  
Vol 67 (1) ◽  
pp. 251-268 ◽  
Author(s):  
Burkhard E Wagner ◽  
John N Helbert ◽  
Edward H Poindexter ◽  
Richard D Bates
Keyword(s):  
Free Radical ◽  
Spin Labels ◽  
Silica Surfaces

Mechanism of Heavy Element Retention in Hydrated Layers Formed on Leached Silicate Glasses

1988 ◽  
Vol 127 ◽  
Author(s):  
J.-C. Petit ◽  
J.-C. Dran ◽  
L. Trotignon ◽  
J.-M. Casabonne ◽  
A. Paccagnella ◽  
...  
Keyword(s):  
Rutherford Backscattering Spectrometry ◽  
Selective Dissolution ◽  
Silicate Glasses ◽  
Heavy Elements ◽  
Nuclear Reaction Analysis ◽  
Aqueous Corrosion ◽  
Silica Surfaces ◽  
Dissolution Behaviour ◽  
The Relationship

ABSTRACTWe have investigated the relationship between hydrated layer formation during aqueous corrosion of silicates and retention of heavy elements (Fe, REE, actinides). Our approach is based on the comparison of the dissolution behaviour of silicate glasses, silicate minerals implanted with increasing doses of lead ions (1×E+12 to 1×E+15 ions/cm2), sorption experiments on silica surfaces and direct precipitation of hydrosilicates. The characterization of reacted surfaces was performed by combining Rutherford backscattering spectrometry (RBS) for profiling heavy elements with Resonant Nuclear Reaction Analysis (RNRA) for hydrogen profilimetry. The accumulation of these elements does not necessarily imply a selective dissolution and can be explained by the “precipitation” of hydroxides or hydrosilicates.

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