QCRI advanced transcription system (QATS) for the Arabic Multi-Dialect Broadcast media recognition: MGB-2 challenge

2016 ◽  
Author(s):  
Sameer Khurana ◽  
Ahmed Ali
Keyword(s):  
Broadcast Media ◽  
Transcription System

International broadcasting: stages of development, specificity and functional structure

2020 ◽  
Vol 4 (4(13)) ◽  
pp. 51-60
Author(s):  
Ksenia Olegovna NEVMERZHITSKAYA ◽  
Keyword(s):  
Foreign Policy ◽  
Global Media ◽  
False Information ◽  
Broadcast Media ◽  
World Community ◽  
Media Agenda ◽  
International Broadcasting ◽  
The World ◽  
The Media ◽  
Media Reports

The media influence politics by providing intelligence and arena for political statements. Therefore, the danger of spreading false information and deliberate disinformation can have serious consequences. It is impossible to accuse specific media outlets of unfair coverage, but one cannot fail to note the existing resonance in media reports from different countries. Interpretations of the same events are radically different, while a journalist must rely on facts. The world is faced with the problem of global misunderstanding and information discord. Modern international broadcasting plays an important role in shaping the picture of the event for the world community. It is impossible to deny that the information agenda of many foreign broadcast media depends to some extent on a number of reasons: nationality, foreign policy of his state, profitability. Otherwise, the global media would not contradict each other. We want to track how modern foreign broadcasting builds its agenda and what principles it is guided by. Keywords: Broadcasting, media, Media agenda

scholarly journals The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Yick W Fong ◽  
Jaclyn J Ho ◽  
Carla Inouye ◽  
Robert Tjian
Keyword(s):  
Stem Cell ◽  
Es Cells ◽  
Embryonic Stem ◽  
In Vitro Transcription ◽  
Specific Gene ◽  
Transcriptional Level ◽  
Ips Cell ◽  
Ribonucleoprotein Complex ◽  
Transcription System

Acquisition of pluripotency is driven largely at the transcriptional level by activators OCT4, SOX2, and NANOG that must in turn cooperate with diverse coactivators to execute stem cell-specific gene expression programs. Using a biochemically defined in vitro transcription system that mediates OCT4/SOX2 and coactivator-dependent transcription of the Nanog gene, we report the purification and identification of the dyskerin (DKC1) ribonucleoprotein complex as an OCT4/SOX2 coactivator whose activity appears to be modulated by a subset of associated small nucleolar RNAs (snoRNAs). The DKC1 complex occupies enhancers and regulates the expression of key pluripotency genes critical for self-renewal in embryonic stem (ES) cells. Depletion of DKC1 in fibroblasts significantly decreased the efficiency of induced pluripotent stem (iPS) cell generation. This study thus reveals an unanticipated transcriptional role of the DKC1 complex in stem cell maintenance and somatic cell reprogramming.

Toward a New Conceptualization of Journalistic Competency: An Analysis of U.S. Broadcasting Job Announcements

2020 ◽  
pp. 107769582092530 ◽  
Author(s):  
Lei Guo ◽  
Yong Volz
Keyword(s):  
Content Analysis ◽  
Professional Values ◽  
Professional Competencies ◽  
Competence Model ◽  
Four Dimensions ◽  
Broadcast Media ◽  
Ethical Competence ◽  
Cognitive Knowledge ◽  
Constitutive Element

Journalistic competency is a constitutive element of professional values and practices in journalism. But what constitutes journalistic competency in today’s ever-changing media landscape? Existing literature lacks theoretical and empirical understandings of journalistic competency, especially in broadcasting. Drawing on Cheetham and Chivers’s competence model, we examine professional competencies as defined by broadcast media through a content analysis of 359 job announcements. Four dimensions of journalistic competency were explicated and empirically assessed: cognitive/knowledge, functional, personal/behavioral, and ethical competence.

scholarly journals The dynamics of gene transcription with a periodic synthesis rate

2021 ◽  
Author(s):  
Qiwen Sun ◽  
Feng Jiao ◽  
Jianshe Yu
Keyword(s):  
Gene Activation ◽  
Transcript Level ◽  
Cyclic Behavior ◽  
Synthesis Rate ◽  
Activation Process ◽  
Transcription System ◽  
Constant Rate ◽  
Real Value ◽  
The Mean ◽  
Two State Model

AbstractThe periodic transcription output is ubiquitously observed in an isogenic cell population. To understand mechanisms of cyclic behavior in transcription, we extend the gene activation process in the two-state model by assuming that the synthesis rate is periodic. We derive the analytical forms of the mean transcript level and the noise. The limits of them indicate that the mean level and the noise display periodic behaviors. Numerical examples strongly suggest that the transcription system with a periodic synthesis rate generates more noise than that with a constant rate but maintains transcription homeostasis in each period. It is also suggested that if the periodicity is not considered, the calculated noise may be greater than the real value.

scholarly journals Cell-Free Transcription at 95°: Thermostability of Transcriptional Components and DNA Topology Requirements of Pyrococcus Transcription

Genetics ◽  
1999 ◽  
Vol 152 (4) ◽  
pp. 1325-1333
Author(s):  
Carina Hethke ◽  
Agnes Bergerat ◽  
Winfried Hausner ◽  
Patrick Forterre ◽  
Michael Thomm
Keyword(s):  
Transcription Factors ◽  
Half Life ◽  
Pyrococcus Furiosus ◽  
Polymerase Activity ◽  
Supercoiled Dna ◽  
Eukaryotic Transcription ◽  
Transcription System ◽  
Archaeal Transcription ◽  
Protein Components

Abstract Cell-free transcription of archaeal promoters is mediated by two archaeal transcription factors, aTBP and TFB, which are orthologues of the eukaryotic transcription factors TBP and TFIIB. Using the cell-free transcription system described for the hyperthermophilic Archaeon Pyrococcus furiosus by Hethke et al., the temperature limits and template topology requirements of archaeal transcription were investigated. aTBP activity was not affected after incubation for 1 hr at 100°. In contrast, the half-life of RNA polymerase activity was 23 min and that of TFB activity was 3 min. The half-life of a 328-nt RNA product was 10 min at 100°. Best stability of RNA was observed at pH 6, at 400 mm K-glutamate in the absence of Mg2+ ions. Physiological concentrations of K-glutamate were found to stabilize protein components in addition, indicating that salt is an important extrinsic factor contributing to thermostability. Both RNA and proteins were stabilized by the osmolyte betaine at a concentration of 1 m. The highest activity for RNA synthesis at 95° was obtained in the presence of 1 m betaine and 400 mm K-glutamate. Positively supercoiled DNA, which was found to exist in Pyrococcus cells, can be transcribed in vitro both at 70° and 90°. However, negatively supercoiled DNA was the preferred template at all temperatures tested. Analyses of transcripts from plasmid topoisomers harboring the glutamate dehydrogenase promoter and of transcription reactions conducted in the presence of reverse gyrase indicate that positive supercoiling of DNA inhibits transcription from this promoter.

scholarly journals Concentration dependence of transcriptional transactivation in inducible E1A-containing human cells.

1988 ◽  
Vol 8 (11) ◽  
pp. 4799-4807 ◽  
Author(s):  
L J Brunet ◽  
A J Berk
Keyword(s):  
Adenovirus Type ◽  
In Vitro Transcription ◽  
Mrna Levels ◽  
Adenovirus E1a ◽  
Transcription System ◽  
Nuclear Extracts ◽  
Tumor Virus ◽  
E1a Gene ◽  
High Level

The adenovirus E1A proteins are essential for the normal temporal activation of transcription from every other adenoviral early promoter. High-level E1A expression in the absence of viral infection would facilitate biochemical studies of E1A-mediated transactivation. Toward this end, we introduced the adenovirus type 2 E1A gene under the control of the murine mammary tumor virus promoter into HeLa cells. Uninduced cells expressed little or no detectable E1A mRNA. Upon induction, mRNA levels accumulated to about 50% of the level observed in 293 cells. The level of E1A expression in these cells could be controlled by varying the concentration of the inducing glucocorticoid. Under these conditions of varying E1A concentrations, it was observed that activation of the E2, E3, and E4 promoters of H5dl312 initiated at the same E1A concentration and that transcription from each promoter increased as the E1A concentration increased. These results indicate that E1A-mediated transactivation is proportional to the concentration of E1A protein. E1A-dependent transcriptional stimulation of the E4 promoter was reproduced in an in vitro transcription system, demonstrating that expression of only the E1A proteins was sufficient to increase the transcriptional activity of nuclear extracts.

Predictors of Advertising Avoidance in Print and Broadcast Media

1997 ◽  
Vol 26 (3) ◽  
pp. 61-76 ◽  
Author(s):  
Paul Surgi Speck ◽  
Michael T. Elliott
Keyword(s):  
Broadcast Media ◽  
Advertising Avoidance
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