Label-free electronic detection of growth factor induced cellular chatter on chemoreceptive neuron MOS (CvMOS) transistors

2009 ◽  
Author(s):  
Krishna Jayant ◽  
Teresa Porri ◽  
Jon. W. Erickson ◽  
Edwin C. Kan
Keyword(s):  
Growth Factor ◽  
Label Free ◽  
Electronic Detection

scholarly journals Label-Free Raman Imaging to Monitor Breast Tumor Signatures

2016 ◽  
Vol 16 (4) ◽  
pp. 461-469 ◽  
Author(s):  
Felicia S. Manciu ◽  
John D. Ciubuc ◽  
Karla Parra ◽  
Marian Manciu ◽  
Kevin E. Bennet ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Structural Characteristics ◽  
Simultaneous Detection ◽  
Epidermal Growth Factor Receptors ◽  
Label Free ◽  
Phosphorylated Akt ◽  
Data Points ◽  
Epidermal Growth

Although not yet ready for clinical application, methods based on Raman spectroscopy have shown significant potential in identifying, characterizing, and discriminating between noncancerous and cancerous specimens. Real-time and accurate medical diagnosis achievable through this vibrational optical method largely benefits from improvements in current technological and software capabilities. Not only is the acquisition of spectral information now possible in milliseconds and analysis of hundreds of thousands of data points achieved in minutes, but Raman spectroscopy also allows simultaneous detection and monitoring of several biological components. Besides demonstrating a significant Raman signature distinction between nontumorigenic (MCF-10A) and tumorigenic (MCF-7) breast epithelial cells, our study demonstrates that Raman can be used as a label-free method to evaluate epidermal growth factor activity in tumor cells. Comparative Raman profiles and images of specimens in the presence or absence of epidermal growth factor show important differences in regions attributed to lipid, protein, and nucleic acid vibrations. The occurrence, which is dependent on the presence of epidermal growth factor, of new Raman features associated with the appearance of phosphothreonine and phosphoserine residues reflects a signal transduction from the membrane to the nucleus, with concomitant modification of DNA/RNA structural characteristics. Parallel Western blotting analysis reveals an epidermal growth factor induction of phosphorylated Akt protein, corroborating the Raman results. The analysis presented in this work is an important step toward Raman-based evaluation of biological activity of epidermal growth factor receptors on the surfaces of breast cancer cells. With the ultimate future goal of clinically implementing Raman-guided techniques for the diagnosis of breast tumors (e.g., with regard to specific receptor activity), the current results just lay the foundation for further label-free optical tools to diagnose the disease.

Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor

2005 ◽  
Vol 44 (34) ◽  
pp. 5456-5459 ◽  
Author(s):  
Yi Xiao ◽  
Arica A. Lubin ◽  
Alan J. Heeger ◽  
Kevin W. Plaxco
Keyword(s):  
Blood Serum ◽  
Label Free ◽  
Electronic Detection

Label-Free Electronic Detection of DNA Using Simple Double-Walled Carbon Nanotube Resistors

2008 ◽  
Vol 112 (26) ◽  
pp. 9891-9895 ◽  
Author(s):  
Xiaochen Dong ◽  
Dongliang Fu ◽  
Yanping Xu ◽  
Jinquan Wei ◽  
Yumeng Shi ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Label Free ◽  
Electronic Detection

Label-free electronic detection of interleukin-6 using horizontally aligned carbon nanotubes

2016 ◽  
Vol 90 ◽  
pp. 852-857 ◽  
Author(s):  
Hu Chen ◽  
Teck Kian Choo ◽  
Jingfeng Huang ◽  
Yi Wang ◽  
Yuanjun Liu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Interleukin 6 ◽  
Label Free ◽  
Aligned Carbon Nanotubes ◽  
Electronic Detection

Label-free aptasensor for platelet-derived growth factor (PDGF) protein

2008 ◽  
Vol 613 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Tesfaye Hailu Degefa ◽  
Juhyoun Kwak
Keyword(s):  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Label Free
Sign in / Sign up

Export Citation Format

Share Document