Electrical recording of lipid membrane in a microfluidic device

TBEV-particles are assembled in an immature, noninfectious form in the endoplasmic reticulum by the envelopment of the viral core (containing the viral RNA) by a lipid membrane associated with two viral proteins, prM and E. Immature particles are transported through the cellular exocytic pathway and conformational changes induced by acidic pH in the trans-Golgi network allow the proteolytic cleavage of prM by furin, a cellular protease, resulting in the release of mature and infectious TBE-virions. The E protein controls cell entry by mediating attachment to as yet ill-defined receptors as well as by low-pH-triggered fusion of the viral and endosomal membrane after uptake by receptor-mediated endocytosis. Because of its key functions in cell entry, the E protein is the primary target of virus neutralizing antibodies, which inhibit these functions by different mechanisms. Although all flavivirus E proteins have a similar overall structure, divergence at the amino acid sequence level is up to 60 percent (e.g. between TBE and dengue viruses), and therefore cross-neutralization as well as (some degree of) cross-protection are limited to relatively closely related flaviviruses, such as those constituting the tick-borne encephalitis serocomplex.

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Alkylation of carboxylic acids in a microfluidic device: kinetics parameters determination, Hammett reaction constant measurement and optimization of preparative experiment

10.3390/ecsoc-13-00198 ◽

2009 ◽

Cited By ~ 1

Author(s):

Christian Rolando ◽

Maël Penhoat ◽

Azarmidokht Gholamipour-Shirazi

Keyword(s):

Carboxylic Acids ◽

Microfluidic Device ◽

Kinetics Parameters ◽

Constant Measurement ◽

Reaction Constant

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Novel Microfluidic Device Integrated with a Fluidic-Capacitor to Mimic Heart Beating for Generation of Functional Liver Organoids

IEEJ Transactions on Sensors and Micromachines ◽

10.1541/ieejsmas.139.209 ◽

2019 ◽

Vol 139 (7) ◽

pp. 209-216

Author(s):

Jiaxu Wu ◽

Yoshikazu Hirai ◽

Ken-ichiro Kamei ◽

Toshiyuki Tsuchiya ◽

Osamu Tabata

Keyword(s):

Microfluidic Device ◽

Heart Beating ◽

Liver Organoids

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Pneumatically-driven Microfluidic Device for Evaluating Active Transport by Kinesin Motor Protein

IEEJ Transactions on Sensors and Micromachines ◽

10.1541/ieejsmas.136.384 ◽

2016 ◽

Vol 136 (9) ◽

pp. 384-389

Author(s):

Kazuya Fujimoto ◽

Hirofumi Shintaku ◽

Hidetoshi Kotera ◽

Ryuji Yokokawa

Keyword(s):

Active Transport ◽

Microfluidic Device ◽

Motor Protein ◽

Kinesin Motor

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A microfluidic device with integrated impedance detection for λ-DNA

MRS Proceedings ◽

10.1557/proc-773-n6.5 ◽

2003 ◽

Vol 773 ◽

Cited By ~ 1

Author(s):

Myung-Il Park ◽

Jonging Hong ◽

Dae Sung Yoon ◽

Chong-Ook Park ◽

Geunbae Im

Keyword(s):

Microfluidic Device ◽

Electrochemical Impedance ◽

Direct Detection ◽

Full Potential ◽

Label Free ◽

Buffer Solution ◽

Detection Systems ◽

Significant Difference ◽

Detection Of Biomolecules ◽

Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

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