Prokaryotic expression of the antigenic site B/C of S gene of porcine transmissible gastroenteritis virus and preparation of its monoclonal antibodies

2011 ◽  
Author(s):  
Xiaohua Nie ◽  
Huanrong Li ◽  
Ran Gao ◽  
Wang Xin ◽  
Defeng Cui ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Prokaryotic Expression ◽  
Antigenic Site ◽  
Transmissible Gastroenteritis Virus ◽  
S Gene ◽  
Gastroenteritis Virus ◽  
Porcine Transmissible Gastroenteritis Virus ◽  
Transmissible Gastroenteritis

scholarly journals Recombinant Canine Coronaviruses Related to Transmissible Gastroenteritis Virus of Swine Are Circulating in Dogs

2008 ◽  
Vol 83 (3) ◽  
pp. 1532-1537 ◽  
Author(s):  
Nicola Decaro ◽  
Viviana Mari ◽  
Marco Campolo ◽  
Alessio Lorusso ◽  
Michele Camero ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
The Other ◽  
Spike Protein ◽  
Transmissible Gastroenteritis Virus ◽  
Type Ii ◽  
Internal Organs ◽  
Systemic Involvement ◽  
Gastroenteritis Virus ◽  
Porcine Transmissible Gastroenteritis Virus ◽  
Transmissible Gastroenteritis

ABSTRACT Four canine coronavirus type II (CCoV-II) strains were identified in the guts and internal organs of pups which had died of acute gastroenteritis. The CCoV-II strains were strictly related to porcine transmissible gastroenteritis virus (TGEV) in the N-terminal domain of the spike protein, whereas in the other parts of the genome, a higher genetic relatedness to recent CCoV-II isolates was observed. Experimental infection of dogs with a TGEV-like isolate induced mild gastroenteritis without any systemic involvement. By virus neutralization tests, antigenic differences between reference and TGEV-like CCoVs were found. Our data support the potential recombinant origin of the TGEV-like CCoVs.

scholarly journals Construction and characterization of recombinant porcine adenovirus serotype 5 expressing the transmissible gastroenteritis virus spike gene

2001 ◽  
Vol 82 (1) ◽  
pp. 183-190 ◽  
Author(s):  
Tamás Tuboly ◽  
Éva Nagy
Keyword(s):  
Clinical Signs ◽  
Secretory Iga ◽  
Transmissible Gastroenteritis Virus ◽  
Recombinant Viruses ◽  
S Gene ◽  
Gastroenteritis Virus ◽  
A Genome ◽  
Serotype 5 ◽  
Transmissible Gastroenteritis ◽  
E3 Region

Five recombinant porcine adenoviruses of serotype 5 (PAdV-5) carrying the full-length or the 5′ 2·2 kb half of the transmissible gastroenteritis virus (TGEV) spike (S) gene were generated by homologous recombination in E. coli strain BJ5183 cells and subsequent transfection of swine testicle cells. The foreign genes were inserted into the E3 region of PAdV-5. One recombinant virus had no deletion in the E3 region, whereas a 1·2 kb fragment was removed from the E3 region in the remainder of the recombinant viruses. One stable construct with a 4·4 kb insertion had a genome size of 109·6% of the wild-type genome, the largest reported for any recombinant adenovirus. Only those viruses that carried the S gene in the left to right orientation expressed the S gene. Three recombinant viruses were tested by oral immunization of pigs and both antibody response and virus shedding were monitored. None of the pigs showed clinical signs and the virus was recovered from rectal swabs until 6–7 days post-infection. Viruses expressing the S gene induced TGEV- and PAdV-5-specific virus-neutralizing antibodies. Moreover, TGEV-specific secretory IgA was detected in the small intestine and in the lungs of the immunized animals.

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