Investigation of an atmospheric pressure 2D-array of microdischarges in air using cross-correlation spectroscopy

2016 ◽  
Author(s):  
G. Nayak ◽  
Y. Du ◽  
R. Brandenburg ◽  
P. J. Bruggeman
Keyword(s):  
Atmospheric Pressure ◽  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
2D Array

Cross-correlation spectroscopy in investigations of filamentary gas discharges at atmospheric pressure

2002 ◽  
Author(s):  
K. V. Kozlov ◽  
V. V. Dobryakov ◽  
A. P. Monyakin ◽  
V. G. Samoilovich ◽  
O. S. Shepeliuk ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Gas Discharges

Progress in the Visualization of Filamentary Gas Discharges. Part 2: Visualization of DC Positive Corona Discharges

2004 ◽  
Vol 7 (1) ◽  
Author(s):  
Jerzy Mizeraczyk ◽  
Seiji Kanazawa ◽  
Toshikazu Ohkubo
Keyword(s):  
Atmospheric Pressure ◽  
Recent Progress ◽  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Dielectric Barrier Discharges ◽  
Corona Discharges ◽  
Dielectric Barrier ◽  
Gas Discharges ◽  
New Applications ◽  
Barrier Discharges

AbstractRecently the filamentary gas discharges at atmospheric pressure, such as dielectric-barrier and corona discharges regained their importance due to numerous new applications. These new applications impose a demand of better understanding of the fundamentals of the filamentary discharges. As a consequence, during the last ten years an essential progress in the diagnostics of the filamentary discharges has been made. The recent progress in the investigations of the dielectric-barrier discharges by cross-correlation spectroscopy was described in Part 1 of this paper. Part 2 is a survey of the recent progress in the visualization of DC positive corona discharges.

Cross-correlation spectroscopy study of the transient spark discharge in atmospheric pressure air

2017 ◽  
Vol 26 (5) ◽  
pp. 055010 ◽  
Author(s):  
Mário Janda ◽  
Tomáš Hoder ◽  
Abdollah Sarani ◽  
Ronny Brandenburg ◽  
Zdenko Machala
Keyword(s):  
Atmospheric Pressure ◽  
Cross Correlation ◽  
Spark Discharge ◽  
Correlation Spectroscopy ◽  
Spectroscopy Study

Cross-Correlation Spectroscopy Applied to the Investigation of Barrier Discharges in N2/O2 Mixtures at Atmospheric Pressure

2005 ◽  
Vol 45 (5-6) ◽  
pp. 338-347 ◽  
Author(s):  
H.-E. Wagner ◽  
R. Brandenburg ◽  
K. V. Kozlov ◽  
A. M. Morozov ◽  
P. Michel
Keyword(s):  
Atmospheric Pressure ◽  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Barrier Discharges

scholarly journals Fluorescence Cross-Correlation Spectroscopy Yields True Affinity and Binding Kinetics of Plasmodium Lactate Transport Inhibitors

2021 ◽  
Vol 14 (8) ◽  
pp. 757
Author(s):  
Iga Jakobowska ◽  
Frank Becker ◽  
Stefano Minguzzi ◽  
Kerrin Hansen ◽  
Björn Henke ◽  
...  
Keyword(s):  
Rate Constants ◽  
Cross Correlation ◽  
Fluorescent Protein ◽  
Correlation Spectroscopy ◽  
Confocal Imaging ◽  
Binding Kinetics ◽  
Acceptor Site ◽  
Lactate Transport ◽  
Entry Site ◽  
Hydrogen Bond Acceptor

Blocking lactate export in the parasitic protozoan Plasmodium falciparum is a novel strategy to combat malaria. We discovered small drug-like molecules that inhibit the sole plasmodial lactate transporter, PfFNT, and kill parasites in culture. The pentafluoro-3-hydroxy-pent-2-en-1-one BH296 blocks PfFNT with nanomolar efficiency but an in vitro selected PfFNT G107S mutation confers resistance against the drug. We circumvented the mutation by introducing a nitrogen atom as a hydrogen bond acceptor site into the aromatic ring of the inhibitor yielding BH267.meta. The current PfFNT inhibitor efficiency values were derived from yeast-based lactate transport assays, yet direct affinity and binding kinetics data are missing. Here, we expressed PfFNT fused with a green fluorescent protein in human embryonic kidney cells and generated fluorescent derivatives of the inhibitors, BH296 and BH267.meta. Using confocal imaging, we confirmed the location of the proposed binding site at the cytosolic transporter entry site. We then carried out fluorescence cross-correlation spectroscopy measurements to assign true Ki-values, as well as kon and koff rate constants for inhibitor binding to PfFNT wildtype and the G107S mutant. BH296 and BH267.meta gave similar rate constants for binding to PfFNT wildtype. BH296 was inactive on PfFNT G107S, whereas BH267.meta bound the mutant protein albeit with weaker affinity than to PfFNT wildtype. Eventually, using a set of PfFNT inhibitor compounds, we found a robust correlation of the results from the biophysical FCCS binding assay to inhibition data of the functional transport assay.

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