Calcium signaling of chondrocytes under osmotic stress and mechanical stimulation

2012 ◽  
Author(s):  
Wen Li ◽  
Miri Park ◽  
Catherine Kirn-Safran ◽  
Liyun Wang ◽  
X. Lucas Lu
Keyword(s):  
Osmotic Stress ◽  
Calcium Signaling ◽  
Mechanical Stimulation

Calcium Signaling of Chondrocytes Under Osmotic Stress and Mechanical Stimulation

2012 ◽  
Author(s):  
Wen Li ◽  
Miri Park ◽  
Catherine Kirn-Safran ◽  
Liyun Wang ◽  
X. Lucas Lu
Keyword(s):  
Extracellular Matrix ◽  
Osmotic Stress ◽  
Calcium Signaling ◽  
Cell Line ◽  
Mechanical Stimulation ◽  
Critical Role ◽  
Mechanical Stimulus ◽  
Primary Cells ◽  
Major Objective ◽  
Highly Sensitive

Chondrocytes play a critical role in cartilage remodeling by mediating the biosynthesis, organization, and modification of extracellular matrix (ECM) [1]. Previous studies showed that chondrocytes are highly sensitive to the surrounding mechanical and osmotic environments [2]. However, how these signals are perceived and transduced by chondrocytes remains unclear. One of the earliest responses of chondrocytes to stimuli is a transient oscillation in intracellular Ca2+ concentration ([Ca2+]i) [3]. The major objective of this study was to investigate and compare the Ca2+ signaling of chondrocytes, including both primary cells and chondrogenic cell line, under mechanical stimulus [4] and osmotic stress. The roles of seven essential pathways in Ca2+ signaling were further examined using pharmacological inhibitors.

732 Relation between Mechanical Stimulation by Localized Deformation and Calcium Signaling Response in Single Isolated Osteocytes

2006 ◽  
Vol 2006.5 (0) ◽  
pp. 263-264
Author(s):  
Yuki AONUMA ◽  
Mototsugu TANAKA ◽  
Taiji ADACHI ◽  
Hiroshi KAMIOKA ◽  
Teruko YAMAMOTO(TAKANO) ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Mechanical Stimulation ◽  
Localized Deformation

scholarly journals Intercellular calcium signaling via gap junctions in glioma cells.

1992 ◽  
Vol 118 (1) ◽  
pp. 195-201 ◽  
Author(s):  
A C Charles ◽  
C C Naus ◽  
D Zhu ◽  
G M Kidder ◽  
E R Dirksen ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Calcium Signaling ◽  
Single Cell ◽  
Mechanical Stimulation ◽  
Glioma Cells ◽  
C6 Glioma Cells ◽  
Dye Coupling ◽  
C6 Cells ◽  
Junction Protein ◽  
Stimulation Of

Calcium signaling in C6 glioma cells in culture was examined with digital fluorescence video microscopy. C6 cells express low levels of the gap junction protein connexin43 and have correspondingly weak gap junctional communication as evidenced by dye coupling (Naus, C. C. G., J. F. Bechberger, S. Caveney, and J. X. Wilson. 1991. Neurosci. Lett. 126:33-36). Transfection of C6 cells with the cDNA encoding connexin43 resulted in clones with increased expression of connexin43 mRNA and protein and increased dye coupling, as well as markedly reduced rates of proliferation (Zhu, D., S. Caveney, G. M. Kidder, and C. C. Naus. 1991. Proc. Natl. Acad. Sci. USA. 88:1883-1887; Naus, C. C. G., D. Zhu, S. Todd, and G. M. Kidder. 1992. Cell Mol. Neurobiol. 12:163-175). Mechanical stimulation of a single cell in a culture of non-transfected C6 cells induced a wave of increased intracellular calcium concentration ([Ca2+]i) that showed little or no communication to adjacent cells. By contrast, mechanical stimulation of a single cell in cultures of C6 clones expressing transfected connexin43 cDNA induced a Ca2+ wave that was communicated to multiple surrounding cells, and the extent of communication was proportional to the level of expression of the connexin43 cDNA. These results provide direct evidence that intercellular Ca2+ signaling occurs via gap junctions. Ca2+ signaling through gap junctions may provide a means for the coordinated regulation of cellular function, including cell growth and differentiation.

scholarly journals Correction: Rapid and Localized Mechanical Stimulation and Adhesion Assay: TRPM7 Involvement in Calcium Signaling and Cell Adhesion

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138281 ◽  
Author(s):  
Wagner Shin Nishitani ◽  
Adriano Mesquita Alencar ◽  
Yingxiao Wang
Keyword(s):  
Cell Adhesion ◽  
Calcium Signaling ◽  
Mechanical Stimulation ◽  
Adhesion Assay

scholarly journals Rapid and Localized Mechanical Stimulation and Adhesion Assay: TRPM7 Involvement in Calcium Signaling and Cell Adhesion

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126440 ◽  
Author(s):  
Wagner Shin Nishitani ◽  
Adriano Mesquita Alencar ◽  
Yingxiao Wang
Keyword(s):  
Cell Adhesion ◽  
Calcium Signaling ◽  
Mechanical Stimulation ◽  
Adhesion Assay

scholarly journals Bidirectional calcium signaling between satellite glial cells and neurons in cultured mouse trigeminal ganglia

2009 ◽  
Vol 6 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Sylvia O. Suadicani ◽  
Pavel S. Cherkas ◽  
Jonathan Zuckerman ◽  
David N. Smith ◽  
David C. Spray ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Sensory Neurons ◽  
Glial Cells ◽  
Mechanical Stimulation ◽  
Calcium Concentration ◽  
Cytosolic Calcium ◽  
Trigeminal Ganglia ◽  
Sensory Ganglia ◽  
Satellite Glial Cells ◽  
First Time

Astrocytes communicate with neurons, endothelial and other glial cells through transmission of intercellular calcium signals. Satellite glial cells (SGCs) in sensory ganglia share several properties with astrocytes, but whether this type of communication occurs between SGCs and sensory neurons has not been explored. In the present work we used cultured neurons and SGCs from mouse trigeminal ganglia to address this question. Focal electrical or mechanical stimulation of single neurons in trigeminal ganglion cultures increased intracellular calcium concentration in these cells and triggered calcium elevations in adjacent glial cells. Similar to neurons, SGCs responded to mechanical stimulation with increase in cytosolic calcium that spread to the adjacent neuron and neighboring glial cells. Calcium signaling from SGCs to neurons and among SGCs was diminished in the presence of the broad-spectrum P2 receptor antagonist suramin (50 μM) or in the presence of the gap junction blocker carbenoxolone (100 μM), whereas signaling from neurons to SGCs was reduced by suramin, but not by carbenoxolone. Following induction of submandibular inflammation by Complete Freund's Adjuvant injection, the amplitude of signaling among SGCs and from SGCs to neuron was increased, whereas the amplitude from neuron to SGCs was reduced. These results indicate for the first time the presence of bidirectional calcium signaling between neurons and SGCs in sensory ganglia cultures, which is mediated by the activation of purinergic P2 receptors, and to some extent by gap junctions. Furthermore, the results indicate that not only sensory neurons, but also SGCs release ATP. This form of intercellular calcium signaling likely plays key roles in the modulation of neuronal activity within sensory ganglia in normal and pathological states.

In Situ Calcium Signaling of Chondrocytes Under Non-Serum and Serum Culture

2013 ◽  
Author(s):  
Yilu Zhou ◽  
Lauren Resutek ◽  
Liyun Wang ◽  
X. Lucas Lu
Keyword(s):  
Calcium Signaling ◽  
Mechanical Stimulation ◽  
Biomechanical Properties ◽  
Cellular Level ◽  
Cartilage Explants ◽  
Serum Free ◽  
Better Than

Chemically defined serum-free medium has been shown to maintain the mechanical properties of cartilage allografts better than serum supplemented medium during long-term in vitro culture [1]. Little is known about this beneficial mechanism at a cellular level. Intracellular calcium ([Ca2+]i) signaling is one of the earliest responses in chondrocytes under mechanical stimulation [2]. It was recently found that calcium signaling is involved in the regulation of chondrocyte morphology changes and its short-term anabolic and catabolic responses under mechanical stimulation [3]. In this study we hypothesized that the beneficial mechanisms of serum-free culture could be indicated by the spatiotemporal features of [Ca2+]i signaling of chondrocytes in situ. We aimed to: (i) compare the in situ spontaneous [Ca2+]i responses of chondrocytes cultured in medium with and without serum; (ii) investigate the correlation between the [Ca2+]i responses of chondrocytes and the biomechanical properties of cartilage explants.

scholarly journals Cataract Progression Associated with Modifications in Calcium Signaling in Human Lens Epithelia as Studied by Mechanical Stimulation

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 369
Author(s):  
Marko Gosak ◽  
Dajana Gojić ◽  
Elena Spasovska ◽  
Marko Hawlina ◽  
Sofija Andjelic
Keyword(s):  
Basement Membrane ◽  
Calcium Signaling ◽  
Mechanical Stimulation ◽  
Intercellular Communication ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Cataract Formation ◽  
Functional Importance ◽  
Anterior Lens Capsule ◽  
Spatiotemporal Changes

Ca2+ homeostasis and signaling disturbances are associated with lens pathophysiology and are involved in cataract formation. Here, we explored the spatiotemporal changes in Ca2+ signaling in lens epithelial cells (LECs) upon local mechanical stimulation, to better understand the LECs’ intercellular communication and its association with cataractogenesis. We were interested in if the progression of the cataract affects the Ca2+ signaling and if modifications of the Ca2+ homeostasis in LECs are associated with different cataract types. Experiments were done on the human postoperative anterior lens capsule (LC) preparations consisting of the monolayer of LECs on the basement membrane. Our findings revealed that the Ca2+ signal spreads radially from the stimulation point and that the amplitude of Ca2+ transients decreases with increasing distance. It is noteworthy that a comparison of signaling characteristics with respect to the degree of cataract progression revealed that, in LCs from more developed cataracts, the Ca2+ wave propagates faster and the amplitudes of Ca2+ signals are lower, while their durations are longer. No differences were identified when comparing LCs with regard to the cataract type. Moreover, experiments with Apyrase have revealed that the Ca2+ signals are not affected by ATP-dependent paracrine communication. Our results indicated that cataract progression is associated with modifications in Ca2+ signaling in LECs, suggesting the functional importance of altered Ca2+ signaling of LECs in cataractogenesis.

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