A silicon lab-on-chip for integrated sample preparation by PCR and DNA analysis by hybridization

2003 ◽  
Author(s):  
A. Fuchs ◽  
H. Jeanson ◽  
P. Claustre ◽  
J.A. Gruss ◽  
F. Revol-Cavalier ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Dna Analysis ◽  
Lab On Chip ◽  
On Chip

Error-Correcting Sample Preparation with Cyberphysical Digital Microfluidic Lab-on-Chip

2016 ◽  
Vol 22 (1) ◽  
pp. 1-29 ◽  
Author(s):  
Sudip Poddar ◽  
Sarmishtha Ghoshal ◽  
Krishnendu Chakrabarty ◽  
Bhargab B. Bhattacharya
Keyword(s):  
Sample Preparation ◽  
Lab On Chip ◽  
On Chip ◽  
Digital Microfluidic

scholarly journals Fast Track DNA Analysis Suite for human identification

2013 ◽  
Author(s):  
Peter T Docker ◽  
Joanna Baker ◽  
Steve Haswell
Keyword(s):  
Microfluidic Chip ◽  
Fast Track ◽  
Dna Analysis ◽  
Human Identification ◽  
Rapid Analysis ◽  
Human Interaction ◽  
Lab On Chip ◽  
Human Dna ◽  
On Chip ◽  
Proof Of Principle

This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.

scholarly journals Advances in Directly Amplifying Nucleic Acids from Complex Samples

Biosensors ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 117 ◽  
Author(s):  
Faye M. Walker ◽  
Kuangwen Hsieh
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Point Of Care ◽  
Nucleic Acid Amplification ◽  
Current Status ◽  
Diagnostic Tools ◽  
Complex Samples ◽  
Lab On Chip ◽  
On Chip ◽  
Effective Visualization

Advances in nucleic acid amplification technologies have revolutionized diagnostics for systemic, inherited, and infectious diseases. Current assays and platforms, however, often require lengthy experimental procedures and multiple instruments to remove contaminants and inhibitors from clinically-relevant, complex samples. This requirement of sample preparation has been a bottleneck for using nucleic acid amplification tests (NAATs) at the point of care (POC), though advances in “lab-on-chip” platforms that integrate sample preparation and NAATs have made great strides in this space. Alternatively, direct NAATs—techniques that minimize or even bypass sample preparation—present promising strategies for developing POC diagnostic tools for analyzing real-world samples. In this review, we discuss the current status of direct NAATs. Specifically, we surveyed potential testing systems published from 1989 to 2017, and analyzed their performances in terms of robustness, sensitivity, clinical relevance, and suitability for POC diagnostics. We introduce bubble plots to facilitate our analysis, as bubble plots enable effective visualization of the performances of these direct NAATs. Through our review, we hope to initiate an in-depth examination of direct NAATs and their potential for realizing POC diagnostics, and ultimately transformative technologies that can further enhance healthcare.

scholarly journals Fast Track DNA Analysis Suite for human identification

2013 ◽  
Author(s):  
Peter T Docker ◽  
Joanna Baker ◽  
Steve Haswell
Keyword(s):  
Microfluidic Chip ◽  
Fast Track ◽  
Dna Analysis ◽  
Human Identification ◽  
Rapid Analysis ◽  
Human Interaction ◽  
Lab On Chip ◽  
Human Dna ◽  
On Chip ◽  
Proof Of Principle

This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.

Numerical Analyses of Microchannels Filling in a Lab-on-Chip Device

2006 ◽  
Author(s):  
Marco Rasponi ◽  
Monica Soncini ◽  
Franco Maria Montevecchi ◽  
Alberto Redaelli
Keyword(s):  
Numerical Model ◽  
Dna Analysis ◽  
Fluid Dynamic ◽  
Contact Angles ◽  
Surface Forces ◽  
Lab On Chip ◽  
Viscous Forces ◽  
Filling Time ◽  
Solid Substrates ◽  
On Chip

A prototype of a Lab-on-Chip (LoC) device manufactured by ST Microelectronics Inc., which is intended to be a diagnostic platform for DNA analysis, has been analyzed. In particular, the dynamics of the filling process was evaluated by means of a 3-D numerical model. Measurements of wettability were also conducted by evaluating the surface tension of the examined liquids and their contact angles on the solid substrates. Two different filling conditions were simulated: pure capillarity and a pressure of 1.5 kPa applied to the inlet. Results in terms of filling time, fluids velocities and percentage of air entrapped in the channels were analyzed. The numerical model revealed the presence of 3.4% of air in the channels (localized in the corner regions), when the pressure of 1.5 kPa was applied. In case of zero pressure, the top corners of the central channel got completely wetted, thus reducing the amount of air to 2.7%. The results showed that capillary forces are dominant during the filling of channels with dimensions smaller than 200 μm. General parameters used to have an insight into the kind of forces leading a fluid-dynamic process are the Reynolds (Re) and Capillary (Ca) numbers, ratios between inertial and viscous forces, and viscous and surface forces, respectively. The computed maximum values in our simulations were Re = 60 and Ca = 0.018, showing the predominance of surface forces on both viscous and, indirectly, inertial ones.

HV-CMOS single-chip electronics platform for lab-on-chip DNA analysis

2016 ◽  
Author(s):  
David L. Sloan ◽  
Benjamin Martin ◽  
Gordon Hall ◽  
Andrew Hakman ◽  
Philip Marshall ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Single Chip ◽  
Lab On Chip ◽  
On Chip

Demand-Driven Multi-Target Sample Preparation on Resource-Constrained Digital Microfluidic Biochips

2022 ◽  
Vol 27 (1) ◽  
pp. 1-21
Author(s):  
Sudip Poddar ◽  
Sukanta Bhattacharjee ◽  
Shao-Yun Fang ◽  
Tsung-Yi Ho ◽  
B. B. Bhattacharya
Keyword(s):  
Sample Preparation ◽  
Lab On Chip ◽  
Microfluidic Biochips ◽  
Target Ratio ◽  
Concentration Factors ◽  
Storage Cells ◽  
On Chip ◽  
Digital Microfluidic ◽  
Storage Units ◽  
Generation Problem

Microfluidic lab-on-chips offer promising technology for the automation of various biochemical laboratory protocols on a minuscule chip. Sample preparation (SP) is an essential part of any biochemical experiments, which aims to produce dilution of a sample or a mixture of multiple reagents in a certain ratio. One major objective in this area is to prepare dilutions of a given fluid with different concentration factors, each with certain volume, which is referred to as the demand-driven multiple-target (DDMT) generation problem. SP with microfluidic biochips requires proper sequencing of mix-split steps on fluid volumes and needs storage units to save intermediate fluids while producing the desired target ratio. The performance of SP depends on the underlying mixing algorithm and the availability of on-chip storage, and the latter is often limited by the constraints imposed during physical design. Since DDMT involves several target ratios, solving it under storage constraints becomes even harder. Furthermore, reduction of mix-split steps is desirable from the viewpoint of accuracy of SP, as every such step is a potential source of volumetric split error. In this article, we propose a storage-aware DDMT algorithm that reduces the number of mix-split operations on a digital microfluidic lab-on-chip. We also present the layout of the biochip with -storage cells and their allocation technique for . Simulation results reveal the superiority of the proposed method compared to the state-of-the-art multi-target SP algorithms.

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