Measurement of Mechanical Properties of Living Cells Using Micro Fingers and AFM Cantilevers

2006 ◽  
Author(s):  
Kenji Inoue ◽  
Daisuke Nishi ◽  
Tomohito Takubo ◽  
Tatsuo Arai
Keyword(s):  
Mechanical Properties ◽  
Living Cells

scholarly journals Anisotropic Mechanical Properties of Living Cells Revealed by Integrated Spinning Disk Confocal and Atomic Force Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 513a
Author(s):  
Yuri M. Efremov ◽  
Mirian Velay-Lizancos ◽  
Daniel M. Suter ◽  
Pablo D. Zavattieri ◽  
Arvind Raman
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spinning Disk ◽  
Anisotropic Mechanical Properties

Determination of Cellular Mechanical Properties by Cell Poking, With an Application to Leukocytes

1990 ◽  
Vol 112 (3) ◽  
pp. 283-294 ◽  
Author(s):  
G. I. Zahalak ◽  
W. B. McConnaughey ◽  
E. L. Elson
Keyword(s):  
Mechanical Properties ◽  
Final Section ◽  
Mechanical Response ◽  
Living Cells ◽  
Micropipette Aspiration ◽  
Current Status ◽  
Technical Description ◽  
Analytical Results ◽  
Simple Models

In this paper we review the cell-poking technique as an approach for investigating the mechanical properties of living cells. We first summarize the rationale for the technique and the mainly qualitative results obtained so far. Then we provide a technical description of the instrument as it is configured at present. This is followed by a discussion of the current status of analytical results available for interpreting cell-poking measurements. In the final section we apply these results to an analysis of unmodulated and modulated lymphocytes and neutrophils, and conclude that the mechanical response of these leukocytes to indentation is not consistent with simple models developed by previous investigators on the basis of micropipette-aspiration experiments.

Mechanical properties of single living cells encapsulated in polyelectrolyte matrixes

2006 ◽  
Vol 124 (4) ◽  
pp. 723-731 ◽  
Author(s):  
Tiziana Svaldo Lanero ◽  
Ornella Cavalleri ◽  
Silke Krol ◽  
Ranieri Rolandi ◽  
Alessandra Gliozzi
Keyword(s):  
Mechanical Properties ◽  
Living Cells ◽  
Single Living Cells ◽  
Single Living

STUDYING THE LOCAL MECHANICAL PROPERTIES OF LIVING CELLS VIA SCANNING ION-CONDUCTING MICROSCOPY

2020 ◽  
pp. 154-156
Author(s):  
A. Vaneev ◽  
N. Savin ◽  
A. Yakovlev ◽  
A. Alova ◽  
S. Lavrushkina ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mechanical Properties ◽  
Actin Cytoskeleton ◽  
Tumor Cells ◽  
Cell Nucleus ◽  
Living Cells ◽  
Mechanisms Of Action ◽  
Human Prostate Cancer ◽  
Before And After ◽  
Ion Conducting

The mechanical properties of PC-3 tumor cells of human prostate cancer before and after exposure to substances acting on the actin cytoskeleton, microtubules, and cell nucleus were measured by scanning ion-conducting microscopy. Changes in local mechanical properties corresponding to the mechanisms of action of these substances were found

scholarly journals Atomic Force Microscopy as a Tool for the Investigation of Living Cells

Medicina ◽  
2013 ◽  
Vol 49 (4) ◽  
pp. 25 ◽  
Author(s):  
Inga Morkvėnaitė-Vilkončienė ◽  
Almira Ramanavičienė ◽  
Arūnas Ramanavičius
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
High Resolution ◽  
Force Spectroscopy ◽  
Living Cells ◽  
Fixation Method ◽  
Cell Preparation ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Fixation

Atomic force microscopy is a valuable and useful tool for the imaging and investigation of living cells in their natural environment at high resolution. Procedures applied to living cell preparation before measurements should be adapted individually for different kinds of cells and for the desired measurement technique. Different ways of cell immobilization, such as chemical fixation on the surface, entrapment in the pores of a membrane, or growing them directly on glass cover slips or on plastic substrates, result in the distortion or appearance of artifacts in atomic force microscopy images. Cell fixation allows the multiple use of samples and storage for a prolonged period; it also increases the resolution of imaging. Different atomic force microscopy modes are used for the imaging and analysis of living cells. The contact mode is the best for cell imaging because of high resolution, but it is usually based on the following: (i) image formation at low interaction force, (ii) low scanning speed, and (iii) usage of “soft,” low resolution cantilevers. The tapping mode allows a cell to behave like a very solid material, and destructive shear forces are minimized, but imaging in liquid is difficult. The force spectroscopy mode is used for measuring the mechanical properties of cells; however, obtained results strongly depend on the cell fixation method. In this paper, the application of 3 atomic force microscopy modes including (i) contact, (ii) tapping, and (iii) force spectroscopy for the investigation of cells is described. The possibilities of cell preparation for the measurements, imaging, and determination of mechanical properties of cells are provided. The applicability of atomic force microscopy to diagnostics and other biomedical purposes is discussed.

scholarly journals Mechanical Properties of Organelles Driven by Microtubule-Dependent Molecular Motors in Living Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18332 ◽  
Author(s):  
Luciana Bruno ◽  
Marcelo Salierno ◽  
Diana E. Wetzler ◽  
Marcelo A. Despósito ◽  
Valeria Levi
Keyword(s):  
Mechanical Properties ◽  
Molecular Motors ◽  
Living Cells

OS1103 Mechanical properties of living cells in transient-state by atomic force microscopy

2012 ◽  
Vol 2012 (0) ◽  
pp. _OS1103-1_-_OS1103-2_
Author(s):  
Yusuke MIZUTANI ◽  
Cai PINGGEN ◽  
Masahiro TSUCHIYA ◽  
Takaharu OKAJIMA
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Transient State ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force
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