The measurement of global shortening as a new parameter to evaluate bone specimen response to uniaxial loading: Length change measurement for bone tissue specimens

2016 ◽  
Author(s):  
Emanuele Rizzuto ◽  
Enrica Urciuoli ◽  
Barbara Peruzzi ◽  
Zaccaria Del Prete
Keyword(s):  
Bone Tissue ◽  
Length Change ◽  
Uniaxial Loading ◽  
Bone Specimen ◽  
Change Measurement ◽  
Tissue Specimens

scholarly journals A Method for Rapid Demineralization of Teeth and Bones

2010 ◽  
Vol 4 (1) ◽  
pp. 223-229 ◽  
Author(s):  
Andrew Cho ◽  
Shigeki Suzuki ◽  
Junko Hatakeyama ◽  
Naoto Haruyama ◽  
Ashok B Kulkarni
Keyword(s):  
Reporter Gene ◽  
Gene Expression Pattern ◽  
Cell Lineage ◽  
Careful Analysis ◽  
Lacz Gene ◽  
Bone Specimen ◽  
Specific Expression ◽  
Temporal Gene Expression ◽  
Gene And Protein Expression ◽  
Tissue Specimens

Tooth and bone specimen require extensive demineralization for careful analysis of cell morphology, as well as gene and protein expression levels. The LacZ gene, which encodes the ß-galactosidase enzyme, is often used as a reporter gene to study gene-structure function, tissue-specific expression by a promoter, cell lineage and fate. This reporter gene is particularly useful for analyzing the spatial and temporal gene expression pattern, by expressing the LacZ gene under the control of a promoter of interest. To analyze LacZ activity, and the expression of other genes and their protein products in teeth and bones, it is necessary to carry out a complete demineralization of the specimen before cutting sections. However, strong acids, such as formic acid used for tooth demineralization, destroy the activities of enzymes including those of ß-galactosidase. Therefore, most protocols currently use mild acids such as 0.1 M ethylene diamine tetra-acetic acid (EDTA) for demineralization of tooth and bone specimen, which require a longer period of treatment for complete demineralization. A method by which hard tissue specimens such as teeth and bones can be rapidly, but gently, decalcified is necessary to save time and effort. Here, we report a suitable method for rapid demineralization of mouse teeth in 0.1M EDTA at 42˚C without any loss of ß-galactosidase activity.

A modified method to culture human osteoblasts from bone tissue specimens using fibrin glue

2002 ◽  
Vol 54 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Martijn van Griensven ◽  
Johannes Zeichen ◽  
Thomas Tschernig ◽  
Andreas Seekamp ◽  
Hans-Christoph Pape
Keyword(s):  
Bone Tissue ◽  
Fibrin Glue ◽  
Human Osteoblasts ◽  
Modified Method ◽  
Tissue Specimens

Dynamic mechanical testing system for Raman microscopy of bone tissue specimens

2005 ◽  
Vol 38 (1-2) ◽  
pp. 101-105 ◽  
Author(s):  
Andrew F. Callender ◽  
William F. Finney ◽  
Michael D. Morris ◽  
Nadder D. Sahar ◽  
David H. Kohn ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Mechanical Testing ◽  
Raman Microscopy ◽  
Testing System ◽  
Dynamic Mechanical ◽  
Dynamic Mechanical Testing ◽  
Tissue Specimens

BONE FORMATION IN HYPERCORTISONISM

1970 ◽  
Vol 63 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Jenifer Jowsey ◽  
B. Lawrence Riggs
Keyword(s):  
Bone Formation ◽  
Bone Turnover ◽  
Bone Tissue ◽  
Present Report ◽  
Tissue Specimens

ABSTRACT Bone formation has been reported to be both low and high in hypercortisonism. The present report describes our efforts to elucidate this controversy. Five patients with hypercortisonism were given tetracycline to evaluate both the level and the rate of formation of new bone tissue. Specimens of bone were taken for biopsy. Measurements showed that the rate of formation is normal but the level is low, while resorption of bone is elevated. The study thus established that the abnormality of bone turnover in this disorder is of a depressed level of formation accompanied by increased resorption.

TRANSIENT STRUCTURAL EVOLUTION UPON THERMAL DEHYDRATION IN LAYERED-HECTORITE NANOPARTICLES

2012 ◽  
Vol 11 (06) ◽  
pp. 1240033 ◽  
Author(s):  
KIMINORI SATO ◽  
KAZUOMI NUMATA ◽  
KOICHIRO FUJIMOTO
Keyword(s):  
Structural Evolution ◽  
Length Change ◽  
Molecular Structures ◽  
Water Molecules ◽  
Open Spaces ◽  
Water Desorption ◽  
Time Resolved ◽  
Change Measurement ◽  
Thermal Pressurization ◽  
Insight Into

To gain an insight into earthquake slip weakening due to thermal pressurization, microscopic molecular structures associated with water desorption are investigated for layered-hectorite nanoparticles by positronium (Ps) annihilation spectroscopy, thermogravimetry and differential thermal analysis (TG–DTA) and time-resolved length-change measurement with high resolution dilatometry. The large macroscopic length change of ~ 6% occurs simultaneously with the formation of open spaces with the size of ~ 7 Å when water molecules are desorbed from the interlayer spaces.

Optical length-change measurement based on an incoherent single-bandpass microwave photonic filter with high resolution

2014 ◽  
Vol 2 (4) ◽  
pp. B35 ◽  
Author(s):  
Ye Deng ◽  
Ming Li ◽  
Ningbo Huang ◽  
Hui Wang ◽  
Ninghua Zhu
Keyword(s):  
High Resolution ◽  
Length Change ◽  
Optical Length ◽  
Change Measurement ◽  
Microwave Photonic
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