An image based quantitative fluorescence immunoassay reader for HbA1c testing: Calibration & repeatability study

2016 ◽  
Author(s):  
Kaushik Basak Chowdhury ◽  
Jayaraj Joseph ◽  
Nethra Reddy ◽  
Jayaraman Kiruthi Vasan ◽  
Mohanasankar Sivaprakasam
Keyword(s):  
Fluorescence Immunoassay ◽  
Quantitative Fluorescence

Portable Quantitative Fluorescence Immunoassay Analyzer

2013 ◽  
Vol 321-324 ◽  
pp. 667-670
Author(s):  
Meng Yuan Xie ◽  
Jun Zhang ◽  
Wen Gu ◽  
Zhe Chen ◽  
Jian Hui Yu
Keyword(s):  
Test Strip ◽  
Clinical Test ◽  
Fluorescence Immunoassay ◽  
C Reactive Protein ◽  
Detection Range ◽  
Fluorescent Marker ◽  
Reactive Protein ◽  
Linear Detection ◽  
Technical Specifications ◽  
Quantitative Fluorescence

The article introduces a portable quantitative fluorescence immunoassay analyzer which is based on the principle of fluorescence immunoassay and immunochromatographic assay, the analyzer can quantitatively detect the concentration of C-reactive protein (CRP) in human blood. After the immune reaction between CRP and fluorescent marker, they gather around the test line on test strip. The analyzer detects the fluorescent of CRP and calculates concentration of CRP in the sample according to standard working curves of the analyzer. The results show that the linear detection range of the CRP concentration by this analyzer is 1~27 mg/L, the technical specifications have reached the requirements of clinical test.

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

2019 ◽  
Vol 13 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Tzonka Godjevargova ◽  
Zlatina Becheva ◽  
Yavor Ivanov ◽  
Andrey Tchorbanov
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Fluorescence Immunoassay ◽  
Magnetic Separator ◽  
Milk Samples

Objectives: Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods: Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results: The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion: A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivan Y. Iourov ◽  
◽  
Ilia V. Soloviev ◽  
Yuri B. Yurov ◽  
Svetlana G. Vorsanova ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Quantitative Fluorescence

scholarly journals Quantitative Fluorescence Microscopy on SARS-CoV-2

2021 ◽  
Vol 120 (3) ◽  
pp. 360a
Author(s):  
Rayna M. Addabbo ◽  
John Kohler ◽  
Isaac Angert ◽  
Yan Chen ◽  
Heather Hanson ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Quantitative Fluorescence

scholarly journals Ball-lens assisted sensitivity improvement of fluorescence immunoassay in microchannels

RSC Advances ◽  
2021 ◽  
Vol 11 (44) ◽  
pp. 27541-27546
Author(s):  
Qingquan Zhang ◽  
Jiajia Li ◽  
Yuting Su ◽  
Xiaoyan Pan ◽  
Hongwei Gai
Keyword(s):  
Fluorescence Immunoassay ◽  
Improvement Method ◽  
Sensitivity Improvement

A contactless and ball-lens assisted sensitivity improvement method was present for the fluorescence or luminescence immunoassay in microchannel.

scholarly journals Development of a multicolor upconversion fluorescence immunoassay for the simultaneous detection of thiamethoxam and dextran by magnetic separation

RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 517-524
Author(s):  
Chuanyong Li ◽  
Wanlin Sun ◽  
Lianrun Huang ◽  
Nana Sun ◽  
Xiude Hua ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Determination ◽  
Magnetic Separation ◽  
Simultaneous Detection ◽  
Fluorescence Immunoassay ◽  
Upconversion Fluorescence

The anti-thiamethoxam and anti-dextran monoclonal antibodies were prepared to develop a multicolor upconversion fluorescence immunoassay for the simultaneous determination of thiamethoxam (544 nm) and dextran (477 nm).

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