Genetically modified mesodermal-derived cells for bone tissue engineering - Alternatives to autografts and allografts for accelerated healing of skeletal defects

2003 ◽  
Vol 22 (5) ◽  
pp. 57-64 ◽  
Author(s):  
M.D. Kofron ◽  
J.X. Zhang ◽  
J.R. Lieverman ◽  
C.T. Laurencin
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Genetically Modified ◽  
Skeletal Defects

Selective laser sintering manufacturing of polycaprolactone bone scaffolds for applications in bone tissue engineering

2015 ◽  
Vol 21 (4) ◽  
pp. 386-392 ◽  
Author(s):  
Alida Mazzoli ◽  
C Ferretti ◽  
A Gigante ◽  
E Salvolini ◽  
M Mattioli-Belmonte
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Design Methodology ◽  
Selective Laser Sintering ◽  
Human Mesenchymal Stem Cells ◽  
Laser Sintering ◽  
Porous Scaffolds ◽  
Content Type ◽  
Skeletal Defects

Purpose – The purpose of this study is to show how selective laser sintering (SLS) manufacturing of bioresorbable scaffolds is used for applications in bone tissue engineering. Design/methodology/approach – Polycaprolactone (PCL) scaffolds were computationally designed and then fabricated via SLS for applications in bone and cartilage repair. Findings – Preliminary biocompatibility data were acquired using human mesenchymal stem cells (hMSCs) assuring a satisfactory scaffold colonization by hMSCs. Originality/value – A promising procedure for producing porous scaffolds for the repair of skeletal defects, in tissue engineering applications, was developed.

Preparation of Mineralized Electrospun PCL/Gelatin Scaffolds via Double Diffusion System

2012 ◽  
Vol 512-515 ◽  
pp. 1740-1745
Author(s):  
Zhen Zhao Guo ◽  
Hong Li ◽  
Wei Cheng Guan ◽  
Bo Xue ◽  
Chang Ren Zhou
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Double Diffusion ◽  
Diffusion System ◽  
Precipitation Reaction ◽  
Tissue Engineering Application ◽  
Skeletal Defects

For successful reconstruction of skeletal defects, a range of materials including ceramics, polymers and their composites have been developed. The goal of our work is to prepare mineralized PCL/gelatin composite scaffolds in a double diffusion system as implants for bone tissue engineering application. Fibrous PCL/gelatin scaffold fabricated via electrospinning followed by immersing into disodium-β-glycerophosphate(β-GP) (10 mg/ml) for 12h were used as substrates for calcium phosphate (CaP) mineralization. The precipitation reaction was biomimetically carried out in a double diffusion system for a week. The CaP minerals precipitated on the scaffold were characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy and high-resolution transmission electron microscopy. The results show that apatite aggregates are combination of HAP, DCPD and ACP. β-GP can effectively promote the formation of CaP crystals. The composite scaffold fabricated in this paper hold promise for use in bone tissue engineering.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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