Detection of Autoimmune Disease Markers by Optical Label-Free Immunosensors

2018 ◽  
Author(s):  
V.A. Bragina ◽  
N.V. Guteneva ◽  
S.L. Znoyko ◽  
B.G. Gorshkov ◽  
A.V. Orlov
Keyword(s):  
Autoimmune Disease ◽  
Label Free ◽  
Disease Markers

Label-free optical bifunctional oligonucleotide probe for homogeneous amplification detection of disease markers

2011 ◽  
Vol 29 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Kejun Feng ◽  
Li-Ping Qiu ◽  
Yifeng Yang ◽  
Zai-Sheng Wu ◽  
Guo-Li Shen ◽  
...  
Keyword(s):  
Oligonucleotide Probe ◽  
Label Free ◽  
Disease Markers

scholarly journals Nanoarchitecture Based SERS for Biomolecular Fingerprinting and Label-Free Disease Markers Diagnosis

2016 ◽  
Vol 49 (12) ◽  
pp. 2725-2735 ◽  
Author(s):  
Sudarson Sekhar Sinha ◽  
Stacy Jones ◽  
Avijit Pramanik ◽  
Paresh Chandra Ray
Keyword(s):  
Label Free ◽  
Disease Markers

scholarly journals Circulating Proteasomes Are Functional and Have a Subtype Pattern Distinct from 20S Proteasomes in Major Blood Cells

2006 ◽  
Vol 52 (11) ◽  
pp. 2079-2086 ◽  
Author(s):  
Annette Zoeger ◽  
Michael Blau ◽  
Karl Egerer ◽  
Eugen Feist ◽  
Burkhardt Dahlmann
Keyword(s):  
Autoimmune Disease ◽  
Human Plasma ◽  
Lupus Erythematosus ◽  
Blood Cells ◽  
Healthy Donor ◽  
Intracellular Protein ◽  
Disease Markers ◽  
Apparent Homogeneity ◽  
Healthy Donors ◽  
Donor Plasma

Abstract Background: 20S proteasomes, the proteolytic core particles of the major intracellular protein degradative pathway, are potential disease markers because they are detectable in human plasma as circulating proteasomes and their concentrations are increased in patients suffering from various diseases. To investigate the origin of circulating proteasomes, we compared some of their features with those of proteasomes isolated from major blood cells. Methods: We isolated circulating proteasomes from the plasma of 2 patients with rheumatoid arthritis and 2 with systemic lupus erythematosus and from human plasma from healthy donors. We purified the proteasomes to apparent homogeneity and then used electron microscopy for imaging and chromatography for subtype spectrum analysis. We compared subtype results with those from 20S proteasomes purified from 4 major blood cell populations. We also tested proteasomes for enzymatic activity and immunosubunit content. Results: Circulating proteasomes from plasma of healthy donors and from patients with autoimmune disease were found to have the same size and shape as erythrocyte proteasomes, be proteolytically active, and contain standard- and immunosubunits. Chromatography revealed 6 circulating proteasome subtype peaks in healthy donor plasma and 7 in patient donor plasma. Proteasomes from erythrocytes had 3 subtype peaks and those of monocytes, T-lymphocytes, and thrombocytes each had 5 different subtype peaks. Conclusion: Circulating proteasomes were intact and enzymatically active in plasma from healthy donors and from patients with autoimmune disease. Because the subtype patterns of circulating proteasomes clearly differ from those of proteasomes from blood cells, these cells cannot be regarded as a major source of circulating proteasomes.

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

Laryngeal Manifestations of Autoimmune Diseases

2020 ◽  
Vol 5 (2) ◽  
pp. 439-456
Author(s):  
Jenny L. Pierce
Keyword(s):  
Autoimmune Disease ◽  
Autoimmune Diseases ◽  
Patient Outcomes ◽  
Correct Diagnosis ◽  
Upper Airway ◽  
Clinical Implications ◽  
Laryngeal Function ◽  
The Past ◽  
Referral Practices ◽  
Timely Referral

Purpose This review article provides an overview of autoimmune diseases and their effects on voice and laryngeal function. Method A literature review was conducted in PubMed. Combinations of the following keywords were used: “autoimmune disease and upper airway,” “larynx,” “cough,” “voice,” “dysphonia,” and “dyspnea.” Precedence was given to articles published in the past 10 years due to recent advances in this area and to review articles. Ultimately, 115 articles were included for review. Results Approximately 81 autoimmune diseases exist, with 18 of those highlighted in the literature as having laryngeal involvement. The general and laryngeal manifestations of these 18 are discussed in detail, in addition to the clinical implications for a laryngeal expert. Conclusions Voice, breathing, and cough symptoms may be an indication of underlying autoimmune disease. However, these symptoms are often similar to those in the general population. Appropriate differential diagnosis and timely referral practices maximize patient outcomes. Guidelines are provided to facilitate correct diagnosis when an autoimmune disease is suspected.

Are pharyngeal pouches a manifestation of autoimmune disease?

1998 ◽  
Vol 23 (3) ◽  
pp. 284-284
Author(s):  
Oko ◽  
Swan ◽  
Bottazzo ◽  
Collins
Keyword(s):  
Autoimmune Disease
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