Improved Transfection Efficiency by the use of Lipofectamine-Modified Plasmid DNA in Laser-Induced Stress Wave-Assisted Gene Transfer: In Vitro Study

2006 ◽  
Author(s):  
Mitsuhiro Terakawa ◽  
Risa Otsuka ◽  
Shunichi Sato ◽  
Yasushi Satoh ◽  
Kunio Takishima ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Stress Wave ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
In Vitro Study ◽  
Vitro Study ◽  
Induced Stress

In vitro gene transfer by the use of laser-induced stress wave: effect of laser irradiation conditions on transfection efficiency

2005 ◽  
Author(s):  
Mitsuhiro Terakawa ◽  
Shunichi Sato ◽  
Makoto Ogura ◽  
Yoshinori Masaki ◽  
Maki Uenoyama ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Laser Irradiation ◽  
Stress Wave ◽  
Transfection Efficiency ◽  
Wave Effect ◽  
Induced Stress

In vitro gene transfer by the application of laser-induced stress wave: effect of cellular heating

2004 ◽  
Author(s):  
Mitsuhiro Terakawa ◽  
Shunichi Sato ◽  
Makoto Ogura ◽  
Kuniaki Nakanishi ◽  
Yoshinori Masaki ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Stress Wave ◽  
Wave Effect ◽  
Induced Stress

scholarly journals In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Keiichi Motoyama ◽  
Yoshihiro Nakashima ◽  
Yukihiko Aramaki ◽  
Fumitoshi Hirayama ◽  
Kaneto Uekama ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Cationic Liposomes ◽  
Nonviral Vector ◽  
Liposomal Membrane ◽  
Transfer Activity ◽  
Potential Use

The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed the highest gene transfer activity in HepG2 cells without any significant cytotoxicity. In addition, γ-CyD enhanced the encapsulation ratio of pDNA with AF-liposomes, and also increased gene transfer activity as the entrapment ratio of pDNA into AF-liposomes was increased. γ-CyD stabilized the liposomal membrane of AF-liposomes and inhibited the release of calcein from AF-liposomes. The stabilizing effect of γ-CyD may be, at least in part, involved in the enhancing gene transfer activity of pDNA/γ-CyD/AF-liposomes. Therefore, these results suggest the potential use of γ-CyD for an enhancer of transfection efficiency of AF-liposomes.

Enhanced Transfection Efficiency in Laser-Induced Stress Wave-Assisted Gene Transfer at Low Laser Fluence by Increasing Pressure Impulse

2008 ◽  
Vol 1 ◽  
pp. 038001 ◽  
Author(s):  
Shinta Takano ◽  
Shunichi Sato ◽  
Mitsuhiro Terakawa ◽  
Hiroshi Asida ◽  
Hideyuki Okano ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Stress Wave ◽  
Laser Fluence ◽  
Transfection Efficiency ◽  
Pressure Impulse ◽  
Induced Stress

In vitro study for laser gene transfer in BHK-21 fibroblast cell line

2009 ◽  
Author(s):  
M. Abdel Aziz ◽  
D. S. Salem ◽  
M. S. Salama ◽  
Y. Badr
Keyword(s):  
Gene Transfer ◽  
Cell Line ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Vitro Study ◽  
Fibroblast Cell Line

In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: effects of stress wave parameters, ambient temperature, and cell type

2006 ◽  
Vol 11 (1) ◽  
pp. 014026 ◽  
Author(s):  
Mitsuhiro Terakawa ◽  
Shunichi Sato ◽  
Hiroshi Ashida ◽  
Kazuya Aizawa ◽  
Maki Uenoyama ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Ambient Temperature ◽  
Stress Wave ◽  
Mammalian Cells ◽  
Stress Waves ◽  
Cell Type ◽  
Wave Parameters ◽  
Induced Stress ◽  
Effects Of Stress

Laser-induced stress wave-assisted gene transfection: improved transfection efficiency with cationic liposome-modified plasmid DNA

2007 ◽  
Author(s):  
Risa Otsuka ◽  
Mitsuhiro Terakawa ◽  
Shunichi Sato ◽  
Yasushi Satoh ◽  
Kunio Takishima ◽  
...  
Keyword(s):  
Stress Wave ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Cationic Liposome ◽  
Induced Stress

Gene transfer by the use of laser-induced stress wave: cell type dependence of transfection efficiency

2005 ◽  
Author(s):  
Mitsuhiro Terakawa ◽  
Shunichi Sato ◽  
Hiroshi Ashida ◽  
Minoru Obara
Keyword(s):  
Gene Transfer ◽  
Stress Wave ◽  
Transfection Efficiency ◽  
Cell Type ◽  
Induced Stress
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