Label-Free Detection of DNA Hybridization Using a Reflective Microfiber Bragg Grating Biosensor With Self-Assembly Technique

2017 ◽  
Vol 35 (16) ◽  
pp. 3354-3359 ◽  
Author(s):  
Dandan Sun ◽  
Tuan Guo ◽  
Bai-Ou Guan
Keyword(s):  
Self Assembly ◽  
Dna Hybridization ◽  
Bragg Grating ◽  
Label Free ◽  
Label Free Detection ◽  
Assembly Technique

scholarly journals Label-free Electrochemical Impedance Spectroscopy Aptasensor for Ultrasensitive Detection of Lung Cancer Biomarker Carcinoembryonic Antigen

2021 ◽  
Vol 9 ◽  
Author(s):  
Yawei Wang ◽  
Lei Chen ◽  
Tiantian Xuan ◽  
Jian Wang ◽  
Xiuwen Wang
Keyword(s):  
Carcinoembryonic Antigen ◽  
Self Assembly ◽  
Electrochemical Impedance ◽  
Reference Electrode ◽  
Electrode System ◽  
Impedance Change ◽  
Label Free ◽  
Working Electrode ◽  
Label Free Detection ◽  
Assembly Technique

In this work, an integrated electrode system consisting of a graphene working electrode, a carbon counter electrode and an Ag/AgCl reference electrode was fabricated on an FR-4 glass fiber plate by a polyethylene self-adhesive mask stencil method combined with a manual screen printing technique. The integrated graphene electrode was used as the base electrode, and AuNPs were deposited on the working electrode surface by cyclic voltammetry. Then, the carcinoembryonic antigen aptamer was immobilized using the sulfhydryl self-assembly technique. The sensor uses [Fe(CN)6]3−/4− as a redox probe for label free detection of carcinoembryonic antigen based on the impedance change caused by the difference in electron transfer rate before and after the binding of carcinoembryonic antigen aptamer and the target carcinoembryonic antigen. The results showed a good linear relationship when the CEA concentration is in the range of 0.2–15.0 ng/ml. The detection limit was calculated to be 0.085 ng/ml (S/N = 3).

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

scholarly journals Label-free detection of morpholino-DNA hybridization using a silicon photonics suspended slab micro-ring resonator

2021 ◽  
pp. 1-1
Author(s):  
Soha Yousuf ◽  
Jongmin Kim ◽  
Ajymurat Orozaliev ◽  
Marcus Dahlem ◽  
Yong-Ak Song ◽  
...  
Keyword(s):  
Silicon Photonics ◽  
Dna Hybridization ◽  
Ring Resonator ◽  
Label Free ◽  
Label Free Detection

Label-Free Detection of DNA Hybridization Utilizing Dual S-Tapered Thin-Core Fiber Interferometer

2019 ◽  
Vol 37 (11) ◽  
pp. 2762-2767 ◽  
Author(s):  
Xu Zhang ◽  
Bo Liu ◽  
Hao Zhang ◽  
Xu Zhang ◽  
Binbin Song ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Label Free ◽  
Label Free Detection ◽  
Core Fiber

Label free detection of DNA hybridization by refractive index tapered fiber biosensor

2010 ◽  
Author(s):  
M. I. Zibaii ◽  
H. Latifi ◽  
E. Ghanati ◽  
M. Gholami ◽  
S. M. Hosseini
Keyword(s):  
Refractive Index ◽  
Dna Hybridization ◽  
Label Free ◽  
Tapered Fiber ◽  
Label Free Detection
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