High-throughput protein structure determination using grid computing

2009 ◽  
Author(s):  
Jason W. Schmidberger ◽  
Blair Bethwaite ◽  
Colin Enticott ◽  
Mark A. Bate ◽  
Steve G. Androulakis ◽  
...  
Keyword(s):  
Protein Structure ◽  
Grid Computing ◽  
High Throughput ◽  
Structure Determination ◽  
Protein Structure Determination

scholarly journals Fast iodide-SAD phasing for high-throughput membrane protein structure determination

2017 ◽  
Vol 3 (5) ◽  
pp. e1602952 ◽  
Author(s):  
Igor Melnikov ◽  
Vitaly Polovinkin ◽  
Kirill Kovalev ◽  
Ivan Gushchin ◽  
Mikhail Shevtsov ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Protein ◽  
High Throughput ◽  
Structure Determination ◽  
Protein Structure Determination ◽  
Membrane Protein Structure ◽  
Sad Phasing ◽  
Membrane Protein Structure Determination

An approach to rapid protein crystallization using nanodroplets

2002 ◽  
Vol 35 (2) ◽  
pp. 278-281 ◽  
Author(s):  
B. D. Santarsiero ◽  
D. T. Yegian ◽  
C. C. Lee ◽  
G. Spraggon ◽  
J. Gu ◽  
...  
Keyword(s):  
Protein Structure ◽  
Drug Discovery ◽  
High Throughput ◽  
Structural Genomics ◽  
Structure Determination ◽  
Protein Crystallization ◽  
Protein Structure Determination ◽  
Automated System ◽  
Order Of Magnitude ◽  
Time Required

An approach that enables up to a two order of magnitude reduction in the amount of protein required and a tenfold reduction in the amount of time required for vapor-diffusion protein crystallization is reported. A prototype high-throughput automated system was used for the production of diffraction-quality crystals for a variety of proteins from a screen of 480 conditions using drop volumes as small as 20 nL. This approach results in a significant reduction in the time and cost of protein structure determination, and allows for larger and more efficient screens of crystallization parameter space. The ability to produce diffraction-quality crystals rapidly with minimal quantities of protein enables high-throughput efforts in structural genomics and structure-based drug discovery.

Automated crystallographic system for high-throughput protein structure determination

2003 ◽  
Vol 59 (7) ◽  
pp. 1138-1144 ◽  
Author(s):  
Joseph S. Brunzelle ◽  
Padram Shafaee ◽  
Xiaojing Yang ◽  
Steve Weigand ◽  
Zhong Ren ◽  
...  
Keyword(s):  
Protein Structure ◽  
High Throughput ◽  
Structure Determination ◽  
Protein Structure Determination
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