High-throughput real-time imaging flow cytometry based on Fourier sampling

2016 ◽  
Author(s):  
Qiang Guo ◽  
Hongwei Chen ◽  
Yuxi Wang ◽  
Minghua Chen ◽  
Sigang Yang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
High Throughput ◽  
Real Time Imaging ◽  
Imaging Flow Cytometry ◽  
Fourier Sampling

Application of single-cell real-time imaging flow cytometry in rapid detection of pathogenic fungi in clinical liquid specimens

2021 ◽  
Vol 9 (2) ◽  
pp. 025004
Author(s):  
Linting Lv ◽  
Li Dong ◽  
Jiajia Zheng ◽  
Tuohutaerbieke Maermaer ◽  
Xiangbo Huang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Real Time ◽  
Rapid Detection ◽  
Pathogenic Fungi ◽  
Real Time Imaging ◽  
Imaging Flow Cytometry

scholarly journals Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254739
Author(s):  
Jennifer J. Labisch ◽  
G. Philip Wiese ◽  
Kalpana Barnes ◽  
Franziska Bollmann ◽  
Karl Pflanz
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
High Throughput ◽  
Lentiviral Vector ◽  
Lentiviral Vectors ◽  
Downstream Processing ◽  
Stability Study ◽  
Imaging Method ◽  
Real Time Imaging ◽  
Infectious Titer

The analysis of the infectious titer of the lentiviral vector samples obtained during upstream and downstream processing is of major importance, however, also the most challenging method to be performed. Currently established methods like flow cytometry or qPCR lack the capability of enabling high throughput sample processing while they require a lot of manual handling. To address this limitation, we developed an immunological real-time imaging method to quantify the infectious titer of anti-CD19 CAR lentiviral vectors with a temporal readout using the Incucyte® S3 live-cell analysis system. The infective titers determined with the Incucyte® approach when compared with the flow cytometry-based assay had a lower standard deviation between replicates and a broader linear range. A major advantage of the method is the ability to obtain titer results in real-time, enabling an optimal readout time. The presented protocol significantly decreased labor and increased throughput. The ability of the assay to process high numbers of lentiviral samples in a high throughput manner was proven by performing a virus stability study, demonstrating the effects of temperature, salt, and shear stress on LV infectivity.

scholarly journals High-throughput multiparametric imaging flow cytometry: toward diffraction-limited sub-cellular detection and monitoring of sub-cellular processes

Cell Reports ◽  
2021 ◽  
Vol 34 (10) ◽  
pp. 108824
Author(s):  
Gregor Holzner ◽  
Bogdan Mateescu ◽  
Daniel van Leeuwen ◽  
Gea Cereghetti ◽  
Reinhard Dechant ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Multiparametric Imaging ◽  
Cellular Processes ◽  
Imaging Flow Cytometry

scholarly journals High‐Throughput Particle Uptake Analysis by Imaging Flow Cytometry

2017 ◽  
Vol 80 (1) ◽  
Author(s):  
Asya Smirnov ◽  
Michael D. Solga ◽  
Joanne Lannigan ◽  
Alison K. Criss
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Particle Uptake ◽  
Imaging Flow Cytometry

Optofluidic time-stretch imaging – an emerging tool for high-throughput imaging flow cytometry

Lab on a Chip ◽  
2016 ◽  
Vol 16 (10) ◽  
pp. 1743-1756 ◽  
Author(s):  
Andy K. S. Lau ◽  
Ho Cheung Shum ◽  
Kenneth K. Y. Wong ◽  
Kevin K. Tsia
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
High Throughput ◽  
Cell Imaging ◽  
Imaging Flow Cytometry ◽  
Single Cell Imaging ◽  
Optical Time ◽  
High Throughput Imaging

Optical time-stretch imaging is now proven for ultrahigh-throughput optofluidic single-cell imaging, at least 10–100 times faster.

scholarly journals Label Free Identification of Peripheral Blood Eosinophils Using High-Throughput Imaging Flow Cytometry

2017 ◽  
Vol 139 (2) ◽  
pp. AB163
Author(s):  
Justyna Piasecka ◽  
Holger Hennig ◽  
Fabian J. Theis ◽  
Paul Rees ◽  
Huw D. Summers ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Peripheral Blood ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Blood Eosinophils ◽  
High Throughput Imaging

scholarly journals High-Throughput Multi-parametric Imaging Flow Cytometry

Chem ◽  
2017 ◽  
Vol 3 (4) ◽  
pp. 588-602 ◽  
Author(s):  
Anandkumar S. Rane ◽  
Justina Rutkauskaite ◽  
Andrew deMello ◽  
Stavros Stavrakis
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Parametric Imaging ◽  
Imaging Flow Cytometry
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