Extending and manipulating long DNA molecules for high resolution analysis-development of a novel nanobiotechnological tool for atomic force microscopy

2002 ◽  
Author(s):  
M. Gad ◽  
M. Machida ◽  
W. Mizutani ◽  
M. Ishikawa
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Atomic Force ◽  
High Resolution Analysis ◽  
Resolution Analysis

scholarly journals Advanced atomic force microscopy-based techniques for nanoscale characterization of switching devices for emerging neuromorphic applications

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
Young-Min Kim ◽  
Jihye Lee ◽  
Deok-Jin Jeon ◽  
Si-Eun Oh ◽  
Jong-Souk Yeo
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Electrostatic Force ◽  
Kelvin Probe ◽  
Force Microscopy ◽  
Atomic Force ◽  
Nanoscale Characterization ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Recognition Errors

AbstractNeuromorphic systems require integrated structures with high-density memory and selector devices to avoid interference and recognition errors between neighboring memory cells. To improve the performance of a selector device, it is important to understand the characteristics of the switching process. As changes by switching cycle occur at local nanoscale areas, a high-resolution analysis method is needed to investigate this phenomenon. Atomic force microscopy (AFM) is used to analyze the local changes because it offers nanoscale detection with high-resolution capabilities. This review introduces various types of AFM such as conductive AFM (C-AFM), electrostatic force microscopy (EFM), and Kelvin probe force microscopy (KPFM) to study switching behaviors.

High-resolution atomic force microscopy of duplex and triplex DNA molecules

2007 ◽  
Vol 18 (22) ◽  
pp. 225102 ◽  
Author(s):  
Dmitry Klinov ◽  
Benjamin Dwir ◽  
Eli Kapon ◽  
Natalia Borovok ◽  
Tatiana Molotsky ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Triplex Dna ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Covalent, sequence-specific attachment of long DNA molecules to a surface using DNA-templated click chemistry

2014 ◽  
Vol 50 (60) ◽  
pp. 8131-8133 ◽  
Author(s):  
Gary R. Abel ◽  
Blessing Huynh Cao ◽  
Jason E. Hein ◽  
Tao Ye
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Click Chemistry ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging ◽  
Specific Attachment ◽  
Novel Method

We present a novel method that covalently and sequence-specifically attaches long DNA molecules to a surface that is compatible with high-resolution atomic force microscopy (AFM) imaging.

scholarly journals Exploring Intramolecular Methyl–Methyl Coupling on a Metal Surface for Edge-Extended Graphene Nanoribbons

2021 ◽  
Vol 03 (02) ◽  
pp. 128-133
Author(s):  
Zijie Qiu ◽  
Qiang Sun ◽  
Shiyong Wang ◽  
Gabriela Borin Barin ◽  
Bastian Dumslaff ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Atomic Force Microscopy ◽  
High Resolution ◽  
Graphene Nanoribbons ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Force Microscopy ◽  
Methyl Methyl ◽  
Atomic Force ◽  
Edge Extension

Intramolecular methyl–methyl coupling on Au (111) is explored as a new on-surface protocol for edge extension in graphene nanoribbons (GNRs). Characterized by high-resolution scanning tunneling microscopy, noncontact atomic force microscopy, and Raman spectroscopy, the methyl–methyl coupling is proven to indeed proceed at the armchair edges of the GNRs, forming six-membered rings with sp3- or sp2-hybridized carbons.

scholarly journals Atomic force microscopy of DNA molecules

FEBS Letters ◽  
1992 ◽  
Vol 301 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jie Yang ◽  
Kunio Takeyasu ◽  
Zhifeng Shao
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Atomic Force

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

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