High-resolution cell manipulation for longstanding load on red blood cells

2016 ◽  
Author(s):  
Chia-Hung Dylan Tsai ◽  
Mitsuhiro Horade ◽  
Hiroaki Ito ◽  
Makoto Kaneko ◽  
Motomu Tanaka
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cell Manipulation ◽  
Resolution Cell

Separation and Manipulation of Particles Using Traveling Wave Dielectrophoretic Force

2009 ◽  
Vol 74 ◽  
pp. 219-222
Author(s):  
Thitima Maturos ◽  
Kata Jaruwongrangsee ◽  
Assawapong Sappat ◽  
Tanom Lomas ◽  
Anurat Wisitsoraat ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Applied Voltage ◽  
Traveling Wave ◽  
Blood Cells ◽  
Electrode Array ◽  
Travelling Wave ◽  
Cell Manipulation ◽  
Mixed Solution ◽  
Traveling Wave Dielectrophoresis ◽  
Parallel Electrode

In this work, we present a device for cell manipulation and separation by using travelling wave dielectophoretic force. The device consists of a 16 parallel electrode array and microchamber. The dielectrophoretic PDMS chamber was fabricated by using standard microfabrication techniques. The Cr/Au parallel electrode array of 100 µm wide and 300 nm thick was patterned on a glass slide by sputtering through microshadow mask. The polystyrene microspheres suspension in de-ionized water and red blood cells in D-mannitol solution were used as tested cells. Cells respond to the electric field in various mechanisms depending on the applied voltage and frequency of the AC signals. For 4.5 µm polystyrene, the traveling wave dielectrophoresis happened when the applied voltage was 10 V, and the frequency of the applied signals was in the range of 50 kHz-700 kHz. For 10 µm polystyrene the twDEP occurred when the applied voltage was 7 V, and frequency was in the range 30 kHz-1MHz. While the red blood cells experienced the twDEP when the applied voltage was 3 V and frequency was in the range 50 kHz-2MHz. The mixed solution containing equal amount of 4.5 and 10 µm microspheres were used for separation test. The big microspheres were moved under twDEP force when the applied voltage was 7 V, and the frequency was in the range of 25 kHz-1MHz while the small microspheres were attached to the electrodes. Therefore, the twDEP device can manipulate and separate the microspheres with different sizes, and it can be further applied for cells selection.

High-resolution X-ray imaging ofPlasmodium falciparum-infected red blood cells

2008 ◽  
Vol 73A (10) ◽  
pp. 949-957 ◽  
Author(s):  
Garth J. Williams ◽  
Eric Hanssen ◽  
Andrew G. Peele ◽  
Mark A. Pfeifer ◽  
Jesse Clark ◽  
...  
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
X Ray ◽  
X Ray Imaging ◽  
Ofplasmodium Falciparum

High-resolution angle-resolved measurements of light scattered at small angles by red blood cells in suspension

2006 ◽  
Vol 45 (9) ◽  
pp. 1964 ◽  
Author(s):  
Ioan Turcu ◽  
Cristian V. L. Pop ◽  
Silvia Neamtu
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells

High-resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

2014 ◽  
Vol 46 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Katarzyna M. Marzec ◽  
Anna Rygula ◽  
Bayden R. Wood ◽  
Stefan Chlopicki ◽  
Malgorzata Baranska
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Spatial Location ◽  
Raman Imaging

Sensitive Detection of Survival of Paroxysmal Nocturnal Hemoglobinuria (PNH) Clones in Red Blood Cells, Granulocytes and Monocytes by High Resolution Multiparameter Flow Cytometry.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3436-3436
Author(s):  
Mayur K Movalia ◽  
Andrea Illingworth
Keyword(s):  
Flow Cytometry ◽  
High Resolution ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Clone Size ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Pnh Clone

Abstract Paroxysmal Nocturnal Hemoglobinuria (PNH) is an acquired clonal stem cell disorder characterized by intravascular hemolysis due to GPI-deficient red blood cells sensitive to complement-mediated lysis. Accurate and sensitive detection of PNH-type cells has become important not only to diagnose PNH but also because studies have shown PNH-type cells may indicate favorable response to therapy and favorable prognosis in patients with aplastic anemia and myelodysplastic syndrome. Previous studies have suggested optimal testing for PNH-type cells by flow cytometry should be limited to within 48 hours after collection of whole blood. Our laboratory has developed a very sensitive and specific high resolution flow cytometric method for detecting PNH-type cells based on testing over 3,000 patients with known PNH, aplastic anemia, myelodysplastic syndromes and other bone marrow failure syndromes. The aim for this study was to determine the longevity of PNH clones in whole blood samples, the day-to-day variability of these clones and the rate of deterioration of the PNH clones compared to normal blood cells. We analyzed 10 whole blood samples from patients known to have PNH-type cells on seven consecutive days utilizing a two-color assay with GPA-CD59 for the red blood cells, a 5-color assay with FLAER-CD24-CD14-CD15-CD45 for the granulocytes and a 5 color assay with FLAER-CD33-CD14-CD64-CD45 for the monocytes. The results are summarized in the table below. The initial PNH clone sizes ranged from 0.02% to 90.8%. The PNH cells showed an overall similar level of deterioration to the normal blood cells with even minor PNH clones of 0.02% able to be detected at day 7. The day-to-day variability of PNH clone sizes was generally less than 10%, with smaller clone sizes showing a higher degree of variation, up to 20%, due to their smaller absolute numbers. Interestingly, Type III PNH red blood cells showed slightly better overall survival than normal red blood cells and were detected in modestly increasing percentages throughout the study. Based on this data, we propose that accurate detection of PNH type cells can be achieved up to seven days after collection of whole blood when utilizing high resolution flow cytometry. PNH Clone Size on Sequential Days as Percentage of Original PNH Clone Size Original PNH Clone Sizes PNH Clone Sizes as Percentage of Original PNH Clone Size Cell Type Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Type III RBCs .02%–58.6% 102% 106% 107% 104% 108% 103% Granulocytes .29%–90.8% 100% 100% 93% 89% 79% 86% Monocytes .52%–89.9% 96% 96% 92% 94% 97% 85%

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