DNA sequence alignment: A review of hardware accelerators and a new core architecture

2016 ◽  
Author(s):  
D. S. Nurdin ◽  
M. N. Isa ◽  
S. H. Goh
Keyword(s):  
Dna Sequence ◽  
Sequence Alignment ◽  
Hardware Accelerators ◽  
Dna Sequence Alignment

scholarly journals DISCo-microbe: design of an identifiable synthetic community of microbes

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8534 ◽  
Author(s):  
Dana L. Carper ◽  
Travis J. Lawrence ◽  
Alyssa A. Carrell ◽  
Dale A. Pelletier ◽  
David J. Weston
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Dna Sequence ◽  
Sequence Alignment ◽  
Amplicon Sequencing ◽  
Ribosomal Database Project ◽  
Community Members ◽  
Dna Sequence Alignment ◽  
Diverse Community

Background Microbiomes are extremely important for their host organisms, providing many vital functions and extending their hosts’ phenotypes. Natural studies of host-associated microbiomes can be difficult to interpret due to the high complexity of microbial communities, which hinders our ability to track and identify individual members along with the many factors that structure or perturb those communities. For this reason, researchers have turned to synthetic or constructed communities in which the identities of all members are known. However, due to the lack of tracking methods and the difficulty of creating a more diverse and identifiable community that can be distinguished through next-generation sequencing, most such in vivo studies have used only a few strains. Results To address this issue, we developed DISCo-microbe, a program for the design of an identifiable synthetic community of microbes for use in in vivo experimentation. The program is composed of two modules; (1) create, which allows the user to generate a highly diverse community list from an input DNA sequence alignment using a custom nucleotide distance algorithm, and (2) subsample, which subsamples the community list to either represent a number of grouping variables, including taxonomic proportions, or to reach a user-specified maximum number of community members. As an example, we demonstrate the generation of a synthetic microbial community that can be distinguished through amplicon sequencing. The synthetic microbial community in this example consisted of 2,122 members from a starting DNA sequence alignment of 10,000 16S rRNA sequences from the Ribosomal Database Project. We generated simulated Illumina sequencing data from the constructed community and demonstrate that DISCo-microbe is capable of designing diverse communities with members distinguishable by amplicon sequencing. Using the simulated data we were able to recover sequences from between 97–100% of community members using two different post-processing workflows. Furthermore, 97–99% of sequences were assigned to a community member with zero sequences being misidentified. We then subsampled the community list using taxonomic proportions to mimic a natural plant host–associated microbiome, ultimately yielding a diverse community of 784 members. Conclusions DISCo-microbe can create a highly diverse community list of microbes that can be distinguished through 16S rRNA gene sequencing, and has the ability to subsample (i.e., design) the community for the desired number of members and taxonomic proportions. Although developed for bacteria, the program allows for any alignment input from any taxonomic group, making it broadly applicable. The software and data are freely available from GitHub (https://github.com/dlcarper/DISCo-microbe) and Python Package Index (PYPI).

scholarly journals Benchmark of algorithms for multiple DNA sequence alignment across livestock species

2021 ◽  
Vol 3 (2) ◽  
pp. 41
Author(s):  
Artur Bąk ◽  
Grzegorz Migdałek ◽  
Chandra Shekhar Pareek ◽  
Kacper Żukowski
Keyword(s):  
Dna Sequence ◽  
Sequence Alignment ◽  
Livestock Species ◽  
Dna Sequence Alignment

scholarly journals A Resistive CAM Processing-in-Storage Architecture for DNA Sequence Alignment

IEEE Micro ◽  
2017 ◽  
Vol 37 (4) ◽  
pp. 20-28 ◽  
Author(s):  
Roman Kaplan ◽  
Leonid Yavits ◽  
Ran Ginosar ◽  
Uri Weiser
Keyword(s):  
Dna Sequence ◽  
Sequence Alignment ◽  
Dna Sequence Alignment
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