Determination of Protoporphyrin IX Concentration in Photodynamic Therapy Based on Fluorescence Measurement

2009 ◽  
Author(s):  
Nadia Naghavi ◽  
Mohammad Hossein Miranbaygi ◽  
Ameneh Sazgarnia
Keyword(s):  
Photodynamic Therapy ◽  
Protoporphyrin Ix ◽  
Fluorescence Measurement

Fluorescence diagnosis of non-melanoma skin cancer

2012 ◽  
Vol 153 (34) ◽  
pp. 1334-1340 ◽  
Author(s):  
Magdolna Gaál ◽  
Róbert Kui ◽  
Zsolt Hunyadi ◽  
Lajos Kemény ◽  
Rolland Gyulai
Keyword(s):  
Photodynamic Therapy ◽  
Protoporphyrin Ix ◽  
Proliferating Cells ◽  
Tumor Margins ◽  
Non Melanoma Skin Cancer ◽  
Skin Cancers ◽  
Exact Determination ◽  
Melanoma Skin

Photodynamic therapy involves – in dermatological practice usually exogenous – application of a photosensitizer then activation of accumulated protoporphyrin IX by light with an appropriate wavelength after a short incubation period. It is an evidence based method to treat certain non-melanoma skin cancers. During treatment when the excited protoporphyrin IX returns to base state, reactive oxygen species are formed leading to cell death in rapidly proliferating cells. Fluorescence of excited protoporphyrin IX can be used in diagnostics as well. In ultraviolet light, the photodamaged or neoplastic areas show coral red fluorescence which can clearly be distinguished from the much lower fluorescence of adjacent normal tissue. This process is suitable for exact determination of tumor margins so it can be used for planning surgical procedures or after photodynamic therapy at a follow up visit for the visualization of the therapeutic result. The present article reviews the literature of photodynamic diagnosi that is also used by the authors. Orv. Hetil., 2012, 153, 1334–1340.

Protoporphyrin IX Fluorescence Photobleaching and the Response of Rat Barrett's Esophagus Following 5-aminolevulinic Acid Photodynamic Therapy

2006 ◽  
Vol 82 (6) ◽  
pp. 1638 ◽  
Author(s):  
Ingrid A. Boere ◽  
Dominic J. Robinson ◽  
Henriette S. de Bruijn ◽  
Jolanda Kluin ◽  
Hugo W. Tilanus ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix

scholarly journals Systematic Review and Meta-Analysis of In Vitro Anti-Human Cancer Experiments Investigating the Use of 5-Aminolevulinic Acid (5-ALA) for Photodynamic Therapy

2021 ◽  
Vol 14 (3) ◽  
pp. 229
Author(s):  
Yo Shinoda ◽  
Daitetsu Kato ◽  
Ryosuke Ando ◽  
Hikaru Endo ◽  
Tsutomu Takahashi ◽  
...  
Keyword(s):  
Systematic Review ◽  
Photodynamic Therapy ◽  
Aminolevulinic Acid ◽  
Human Cancer ◽  
Meta Analysis ◽  
Protoporphyrin Ix ◽  
Cell Types ◽  
Amino Acid Derivative ◽  
Photodynamic Diagnosis

5-Aminolevulinic acid (5-ALA) is an amino acid derivative and a precursor of protoporphyrin IX (PpIX). The photophysical feature of PpIX is clinically used in photodynamic diagnosis (PDD) and photodynamic therapy (PDT). These clinical applications are potentially based on in vitro cell culture experiments. Thus, conducting a systematic review and meta-analysis of in vitro 5-ALA PDT experiments is meaningful and may provide opportunities to consider future perspectives in this field. We conducted a systematic literature search in PubMed to summarize the in vitro 5-ALA PDT experiments and calculated the effectiveness of 5-ALA PDT for several cancer cell types. In total, 412 articles were identified, and 77 were extracted based on our inclusion criteria. The calculated effectiveness of 5-ALA PDT was statistically analyzed, which revealed a tendency of cancer-classification-dependent sensitivity to 5-ALA PDT, and stomach cancer was significantly more sensitive to 5-ALA PDT compared with cancers of different origins. Based on our analysis, we suggest a standardized in vitro experimental protocol for 5-ALA PDT.

Determination of the minimum suppressive concentration of photosensitizers of the second generation in relation to sanitaryindicative microorganisms

2021 ◽  
Vol 1 (11) ◽  
pp. 42-46
Author(s):  
Vadim Yu. Shlenskiy ◽  
◽  
Tatiana I. Pashnik ◽  
Svetlana Yu. Pigina ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Second Generation ◽  
Research Work ◽  
Protoporphyrin Ix ◽  
Bactericidal Effect ◽  
Disodium Salt ◽  
Bacterial Cultures

In the studies described in the article, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed. In the experiments, photosensitizers of the second generation were used: «Photoditazine», the active substance of which is dimeglumin chloride E6, and «Dimegin», which includes a derivative of protoporphyrin IX-2,4-di(1-methoxyethyl)- deuteroporphyrin IX disodium salt. In the described research work, the minimum concentrations of the studied drugs were determined by the method of sequential tenfold dilutions to obtain a bactericidal effect on strains of sanitary-indicative microorganisms Enterococcus faecalis, Staphylococcus aureus, Salmonella enteritidis.

Electron capture by some molecules of biological significance and the determination of absolute electron affinities

1968 ◽  
Vol 21 (12) ◽  
pp. 2853 ◽  
Author(s):  
A Fulton ◽  
LE Lyons ◽  
GC Morris
Keyword(s):  
Electron Capture ◽  
Protoporphyrin Ix ◽  
Biological Significance ◽  
Molecular Orbitals ◽  
Electron Affinities ◽  
Metal Free ◽  
Capture Method

The electron capture method was applied to protoporphyrin IX and meso- porphyrin IX dimethyl esters, metal-free phthalocyanine, chlorophyll, riboflavine, nicotinamide, tetracene, and pentacene. The energy quantities obtained correlated with calculated energies of the lowest empty molecular orbitals of these molecules.

scholarly journals eEF1A1 binds and enriches protoporphyrin IX in cancer cells in 5-aminolevulinic acid based photodynamic therapy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Zhichao Fan ◽  
Xiaojun Cui ◽  
Dan Wei ◽  
Wei Liu ◽  
Buhong Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix
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