Serum Metabonomics Study of Systemic Lupus Erythematosus by Rapid Resolution Liquid Chromatography Coupled with Q-TOF Mass Spectrometry

2012 ◽  
Author(s):  
Xinghong Ding ◽  
Ying Yu
Keyword(s):  
Mass Spectrometry ◽  
Systemic Lupus Erythematosus ◽  
Liquid Chromatography ◽  
Lupus Erythematosus ◽  
Systemic Lupus ◽  
Rapid Resolution

scholarly journals Metabolic Analysis of Potential Key Genes Associated with Systemic Lupus Erythematosus Using Liquid Chromatography-Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Li Zeng ◽  
Nian Chen ◽  
Junlin Liao ◽  
Xu Shen ◽  
Shenghua Song ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Systemic Lupus Erythematosus ◽  
Liquid Chromatography ◽  
Lupus Erythematosus ◽  
Liquid Chromatography Mass Spectrometry ◽  
Systemic Lupus ◽  
Ampk Signaling ◽  
Chromatography Mass Spectrometry ◽  
Tyrosine Metabolism

The biological mechanism underlying the pathogenesis of systemic lupus erythematosus (SLE) remains unclear. In this study, we found 21 proteins upregulated and 38 proteins downregulated by SLE relative to normal protein metabolism in our samples using liquid chromatography-mass spectrometry. By PPI network analysis, we identified 9 key proteins of SLE, including AHSG, VWF, IGF1, ORM2, ORM1, SERPINA1, IGF2, IGFBP3, and LEP. In addition, we identified 4569 differentially expressed metabolites in SLE sera, including 1145 reduced metabolites and 3424 induced metabolites. Bioinformatics analysis showed that protein alterations in SLE were associated with modulation of multiple immune pathways, TP53 signaling, and AMPK signaling. In addition, we found altered metabolites associated with valine, leucine, and isoleucine biosynthesis; one carbon pool by folate; tyrosine metabolism; arginine and proline metabolism; glycine, serine, and threonine metabolism; limonene and pinene degradation; tryptophan metabolism; caffeine metabolism; vitamin B6 metabolism. We also constructed differently expressed protein-metabolite network to reveal the interaction among differently expressed proteins and metabolites in SLE. A total of 481 proteins and 327 metabolites were included in this network. Although the role of altered metabolites and proteins in the diagnosis and therapy of SLE needs to be further investigated, the present study may provide new insights into the role of metabolites in SLE.

MO430MASS-SPECTROMETRIC IDENTIFICATION OF POST-TRANSLATIONAL GUANIDINYLATED PROTEINS IN THE CONTEXT OF SYSTEMIC LUPUS ERYTHEMATOSUS

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Juliane Hermann ◽  
Ute Raffetseder ◽  
Michaela Lellig ◽  
Joachim Jankowski ◽  
Vera Jankowski
Keyword(s):  
Mass Spectrometry ◽  
Systemic Lupus Erythematosus ◽  
High Resolution ◽  
Lupus Erythematosus ◽  
Imaging Techniques ◽  
Mass Spectrometric ◽  
Mass Spectrometric Imaging ◽  
Systemic Lupus ◽  
Notch 3 ◽  
Resolution Mass

Abstract Background and Aims With continuous identification of post-translational modified isoforms of proteins, it is becoming increasingly clear that post-translational modifications limit or modify the biological functions of native proteins are majorly involved in development of various chronic disease. This is mostly due to technically advanced molecular identification and quantification methods, mainly based on mass spectrometry. Mass spectrometry has become one of the most powerful tools for the identification of lipids. Method In this study, we used sophisticated high-resolution mass-spectrometric methods to analyze the soluble ligand of receptor Notch-3, namely the Y-box protein (YB)-1, in serum from systemic lupus erythematosus (SLE) patients. In addition, kidneys of lupus-prone (MRL.lpr) mice were analyzed by mass-spectrometric imaging techniques to identify the underlying pathomechanisms. Serum YB-1 was isolated by chromatographic methods, afterwards digested by trypsin and analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The kidneys were fixed in paraffin, then kidney sections were deparaffinized, tryptic digested and analyzed by mass-spectrometric imaging techniques. Mass-spectrometry of extracellular YB-1 in SLE patient serum revealed post-translational guanidinylation of two lysine’s within the highly conserved cold shock domain (CSD) of the YB-1 protein (YB-1-2G). Patients with increased disease activity and those with active renal involvement (lupus nephritis, LN) had a higher degree of dual-guanidinylation within the CSD. Of note, at least one of these modifications was present in all analyzed LN patients, whereas single-guanidinylated YB-1 was present in only one and double modification in none of the control individuals. Mass-spectrometric imaging analyses specifically localized YB-1-2G and increases Notch-3 expression in kidney sections from MRL.lpr mice. Results The data from this study clearly demonstrate the high potential of high-resolution mass spectrometric methods as well as mass spectrometric imaging techniques to identify pathomechanisms of diseases like SLE/LN.

scholarly journals Biomarkers of systemic lupus erythematosus identified using mass spectrometry-based proteomics: a systematic review

2016 ◽  
Vol 21 (5) ◽  
pp. 993-1012 ◽  
Author(s):  
Orthodoxia Nicolaou ◽  
Andreas Kousios ◽  
Andreas Hadjisavvas ◽  
Bernard Lauwerys ◽  
Kleitos Sokratous ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Systematic Review ◽  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Systemic Lupus
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