Kinetic Model of Enzymatic Hydrolysis of Protein about the Protein of Silkworm Pupae -- Alcalase System

Author(s):  
Zhongxing Zhao ◽  
Dankui Liao ◽  
Jianhua Sun ◽  
Zhangfa Tong ◽  
Kelin Huang ◽  
...  
2012 ◽  
Vol 87 (2) ◽  
pp. 1280-1285 ◽  
Author(s):  
Greta Radeva ◽  
Ivo Valchev ◽  
Stoiko Petrin ◽  
Eva Valcheva ◽  
Petya Tsekova

1998 ◽  
Vol 31 (12) ◽  
pp. 3846-3851 ◽  
Author(s):  
Mariastella Scandola ◽  
Maria Letizia Focarete ◽  
Giovanna Frisoni

2001 ◽  
Vol 47 (5) ◽  
pp. 397-403 ◽  
Author(s):  
T Chatterjee ◽  
B K Chatterjee ◽  
D K Bhattacharyya

Studies of the hydrolysis of bornyl, citronellyl, geranyl, and terpenyl acetates by commercially available lipases of Candida rugosa, Rhizopus arrhizus, and Mucor miehei are presented. The hydrolysis of these monoterpene esters is investigated at various temperatures and pHs, and the time dependence of the percentage of esters hydrolysed is studied. The catalytic activities of the lipases in hydrolysing the esters are compared and, overall, it is observed that under the experimental conditions used the nonspecific lipase from C. rugosa produces the highest yields of the monoterpene alcohols in comparison to the primary-ester specific lipases such as R. arrhizus and M. miehei. A rate kinetic model has been used to understand the time dependence of the yield of the product acid.Key words: enzymatic hydrolysis, kinetics, lipase, monoterpene esters.


2019 ◽  
Vol 11 (12) ◽  
pp. 1706-1710
Author(s):  
Yiping Xia ◽  
Hao Yu ◽  
Yaoguang Zhong

In this study, in order to study the kinetic mechanism of enzymatic hydrolysis of salmon protein, the kinetic model of enzymatic hydrolysis of salmon skin protein by papain was established. The skin protein of salmon was hydrolyzed by papain under the following conditions: the mass concentration of salmon skin protein is 55 g/L, the initial papain concentration is 2.0 g/L, the pH of enzymatic solution is 7.2 and the temperature of enzymatic hydrolysis is 55 °C. Finally, the kinetic model of hydrolysis was established as follows: Hydrolysis rate R =(27.217E0–0 0357S0exp[–0.2587(DH)]; Degree of hydrolysis DH = 3.879 ln[1 + 7.0165E0/S0 –0.0092t]. The reaction rate constant k3 = 27.217 min–1 and the enzyme deactivation constant kd = 7.0752 min–1 were deduced to control the enzymatic hydrolysis process. Further verification tests showed that the theoretical value of the degree of hydrolysis of the model was basically consistent with the actual value, and the kinetic model had certain practical value, indicating that the established salmon skin protease kinetic model could be used to guide and optimize the enzymatic hydrolysis process.


2003 ◽  
Vol 13 (1) ◽  
pp. 43-52 ◽  
Author(s):  
C Brandam ◽  
X.M Meyer ◽  
J Proth ◽  
P Strehaiano ◽  
H Pingaud

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