Interactions of Prostate Cancer Cells to Human Microvascular Endothelial Cells Under Shear Stress Conditions

2007 ◽  
Author(s):  
Hao Xu ◽  
Nene Kalu ◽  
Pavithra Raghavan ◽  
Myoung H. Kim ◽  
Kytai Truong Nguyen
Keyword(s):  
Prostate Cancer ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Cancer Cells ◽  
Stress Conditions ◽  
Microvascular Endothelial Cells ◽  
Prostate Cancer Cells ◽  
Microvascular Endothelial

Shear stress increases expression of a KATP channel in rat and bovine pulmonary vascular endothelial cells

2003 ◽  
Vol 285 (4) ◽  
pp. C959-C967 ◽  
Author(s):  
Shampa Chatterjee ◽  
Abu-Bakr Al-Mehdi ◽  
Irena Levitan ◽  
Troy Stevens ◽  
Aron B. Fisher
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Membrane Depolarization ◽  
Microvascular Endothelial Cells ◽  
Adaptation Period ◽  
Pulmonary Microvascular Endothelial Cells ◽  
Channel Expression ◽  
Microvascular Endothelial ◽  
Flow Adaptation ◽  
Expression And Activity

We have shown previously that acute ischemia leads to depolarization of pulmonary microvascular endothelial cells that is prevented with cromakalim, suggesting the presence of ATP-sensitive K+ (KATP) channels in these cells. Thus KATP channel expression and activity were evaluated in rat pulmonary microvascular endothelial cells (RPMVEC) by whole cell current measurements, dot blot (mRNA), and immunoblot (protein) for the inwardly rectifying K+ channel (KIR) 6.2 subunit and fluorescent ligand binding for the sulfonylurea receptor (SUR). Low-level expression of a KATP channel was detected in endothelial cells in routine (static) culture and led us to examine whether its expression is inducible when endothelial cells are adapted to flow. Channel expression (mRNA and both KIR6.2 and SUR proteins) and inwardly rectified membrane current by patch clamp increased significantly when RPMVEC were adapted to flow at 10 dyn/cm2 for 24 h in either a parallel plate flow chamber or an artificial capillary system. Induction of the KATP channel with flow adaptation was also observed in bovine pulmonary artery endothelial cells. Flow-adapted but not static RPMVEC showed cellular plasma membrane depolarization upon stop of flow that was inhibited by a KATP channel opener and prevented by addition of cycloheximide to the medium during the flow adaptation period. These studies indicate the induction of KATP channels by flow adaptation in pulmonary endothelium and that the expression and activity of this channel are essential for the endothelial cell membrane depolarization response with acute decrease in shear stress.

scholarly journals Human brain microvascular endothelial cells resist elongation due to shear stress

2015 ◽  
Vol 99 ◽  
pp. 8-18 ◽  
Author(s):  
Adam Reinitz ◽  
Jackson DeStefano ◽  
Mao Ye ◽  
Andrew D. Wong ◽  
Peter C. Searson
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Human Brain ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2850
Author(s):  
Marta Stojak ◽  
Magdalena Milczarek ◽  
Anna Kurpinska ◽  
Joanna Suraj-Prazmowska ◽  
Patrycja Kaczara ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Endothelial Cells ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Transendothelial Migration ◽  
Microvascular Endothelial Cells ◽  
Protein Disulphide Isomerase ◽  
Cellular Bioenergetics ◽  
Microvascular Endothelial

Cancer cell cross-talk with the host endothelium plays a crucial role in metastasis, but the underlying mechanisms are still not fully understood. We studied the involvement of protein disulphide isomerase A1 (PDIA1) in human breast cancer cell (MCF-7 and MDA-MB-231) adhesion and transendothelial migration. For comparison, the role of PDIA1 in proliferation, migration, cell cycle and apoptosis was also assessed. Pharmacological inhibitor, bepristat 2a and PDIA1 silencing were used to inhibit PDIA1. Inhibition of PDIA1 by bepristat 2a markedly decreased the adhesion of breast cancer cells to collagen type I, fibronectin and human lung microvascular endothelial cells. Transendothelial migration of breast cancer cells across the endothelial monolayer was also inhibited by bepristat 2a, an effect not associated with changes in ICAM-1 expression or changes in cellular bioenergetics. The silencing of PDIA1 produced less pronounced anti-adhesive effects. However, inhibiting extracellular free thiols by non-penetrating blocker p-chloromercuribenzene sulphonate substantially inhibited adhesion. Using a proteomic approach, we identified that β1 and α2 integrins were the most abundant among all integrins in breast cancer cells as well as in lung microvascular endothelial cells, suggesting that integrins could represent a target for PDIA1. In conclusion, extracellular PDIA1 plays a major role in regulating the adhesion of cancer cells and their transendothelial migration, in addition to regulating cell cycle and caspase 3/7 activation by intracellular PDIA1. PDIA1-dependent regulation of cancer–endothelial cell interactions involves disulphide exchange and most likely integrin activation but is not mediated by the regulation of ICAM-1 expression or changes in cellular bioenergetics in breast cancer or endothelial cells.

scholarly journals Gap junction-mediated transfer of miR-145-5p from microvascular endothelial cells to colon cancer cells inhibits angiogenesis

Oncotarget ◽  
2016 ◽  
Vol 7 (19) ◽  
pp. 28160-28168 ◽  
Author(s):  
Dominique Thuringer ◽  
Gaetan Jego ◽  
Kevin Berthenet ◽  
Arlette Hammann ◽  
Eric Solary ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Endothelial Cells ◽  
Gap Junction ◽  
Cancer Cells ◽  
Microvascular Endothelial Cells ◽  
Colon Cancer Cells ◽  
Microvascular Endothelial

scholarly journals VEGF induces expression of Bcl-2 and multiple signaling factors in microvascular endothelial cells in a prostate cancer model

2009 ◽  
Vol 27 (5) ◽  
pp. 659-666 ◽  
Author(s):  
Yoshihisa Sakai ◽  
Steve Goodison ◽  
Wengang Cao ◽  
Virginia Urquidi ◽  
Kazunori Namiki ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Cancer Model ◽  
Microvascular Endothelial ◽  
Multiple Signaling ◽  
Prostate Cancer Model

Bi-directional interactions of prostate cancer cells and bone marrow endothelial cells in three-dimensional culture

The Prostate ◽  
2005 ◽  
Vol 64 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Jeffrey M. Barrett ◽  
Kathy A. Mangold ◽  
Tamas Jilling ◽  
Karen L. Kaul
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Endothelial Cells ◽  
Cancer Cells ◽  
Three Dimensional ◽  
Prostate Cancer Cells ◽  
Three Dimensional Culture
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