Three dimensional graphene scaffold for cardiac tissue engineering and in-situ electrical recording

2016 ◽  
Author(s):  
S. K. Ameri ◽  
P. K. Singh ◽  
R. D'Angelo ◽  
W. Stoppel ◽  
L. Black ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Electrical Recording

scholarly journals Physiological aspects of cardiac tissue engineering

2012 ◽  
Vol 303 (2) ◽  
pp. H133-H143 ◽  
Author(s):  
Thomas Eschenhagen ◽  
Alexandra Eder ◽  
Ingra Vollert ◽  
Arne Hansen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Myocyte ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Basic Research ◽  
Cardiac Tissue Engineering ◽  
Predictive Toxicology

Cardiac tissue engineering aims at repairing the diseased heart and developing cardiac tissues for basic research and predictive toxicology applications. Since the first description of engineered heart tissue 15 years ago, major development steps were directed toward these three goals. Technical innovations led to improved three-dimensional cardiac tissue structure and near physiological contractile force development. Automation and standardization allow medium throughput screening. Larger constructs composed of many small engineered heart tissues or stacked cell sheet tissues were tested for cardiac repair and were associated with functional improvements in rats. Whether these approaches can be simply transferred to larger animals or the human patients remains to be tested. The availability of an unrestricted human cardiac myocyte cell source from human embryonic stem cells or human-induced pluripotent stem cells is a major breakthrough. This review summarizes current tissue engineering techniques with their strengths and limitations and possible future applications.

Analysis of Gene Expression and Morphology of P19 Cells Cultured in an Apatite-Fiber Scaffold

2012 ◽  
Vol 529-530 ◽  
pp. 370-373 ◽  
Author(s):  
Hide Ishii ◽  
Yuya Mukai ◽  
Mamoru Aizawa ◽  
Nobuyuki Kanzawa
Keyword(s):  
Heart Failure ◽  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Cultured Cells ◽  
Three Dimensional ◽  
Heart Tissue ◽  
Cardiac Tissue Engineering ◽  
Cardiac Differentiation ◽  
Embryonal Carcinoma Cell ◽  
Cells Cultured

Heart disease is the second most common cause of mortality in Japan. Most cases of late stage heart failure can only be effectively treated by a heart transplant. Cardiac tissue engineering is emerging both as a new approach for improving the treatment of heart failure and for developing new cardiac drugs. Apatite-fiber scaffold (AFS) was originally designed as a substitute material for bone. AFS contains two sizes of pores and is appropriate for the three dimensional proliferation and differentiation of osteoblasts. To establish engineered heart tissue, a pluripotent embryonal carcinoma cell line, P19.CL6, was cultured in AFS. P19.CL6 cells seeded into AFS proliferated well. Generally, cardiac differentiation of P19.CL6 cells is induced by treating suspension-cultured cells with dimethyl sulfoxide (DMSO), after which the cells form spheroids. However, our results showed that P19.CL6 cells cultured in AFS differentiated into myocytes without forming spheroidal aggregates, and could be cultured for at least one month. Thus, we conclude that AFS is a good candidate as a scaffold for cardiac tissue engineering.

Three-Dimensional Cardiac Tissue Engineering Using a Thermoresponsive Artificial Extracellular Matrix

ASAIO Journal ◽  
2004 ◽  
Vol 50 (4) ◽  
pp. 344-348 ◽  
Author(s):  
Hiroshi Naito ◽  
Yoshiaki Takewa ◽  
Toshihide Mizuno ◽  
Shoji Ohya ◽  
Yasuhide Nakayama ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Artificial Extracellular Matrix

Cardiac tissue engineering: characteristics of in unison contracting two- and three-dimensional neonatal rat ventricle cell (co)-cultures

Biomaterials ◽  
2002 ◽  
Vol 23 (24) ◽  
pp. 4793-4801 ◽  
Author(s):  
M.J.A van Luyn ◽  
R.A Tio ◽  
X.J Gallego y van Seijen ◽  
J.A Plantinga ◽  
L.F.M.H de Leij ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Neonatal Rat ◽  
Engineering Characteristics ◽  
Rat Ventricle

A conductive PEDOT/alginate porous scaffold as a platform to modulate the biological behaviors of brown adipose-derived stem cells

2020 ◽  
Vol 8 (11) ◽  
pp. 3173-3185 ◽  
Author(s):  
Boguang Yang ◽  
Fanglian Yao ◽  
Lei Ye ◽  
Tong Hao ◽  
Yabin Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Adipose Derived Stem Cells ◽  
Brown Adipose ◽  
Myocardial Differentiation

The development of three-dimensional conductive scaffolds is vital to support the adhesion, proliferation and myocardial differentiation of stem cells in cardiac tissue engineering.

scholarly journals Three-dimensional graphene foam as a conductive scaffold for cardiac tissue engineering

2019 ◽  
Vol 34 (1) ◽  
pp. 74-85 ◽  
Author(s):  
Sajad Bahrami ◽  
Nafiseh Baheiraei ◽  
Majid Mohseni ◽  
Mehdi Razavi ◽  
Atefeh Ghaderi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Graphene Foam

Abstract 332: Nanostructure-Templated Alignment of Cardiomyocytes for 3-Dimensional Cardiac Tissue Engineering

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Eduard Sleep ◽  
Jason Mantei ◽  
Mark McClendon ◽  
Eneda Hoxha ◽  
Raj Kishore ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Connexin 43 ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Ips Cell ◽  
3 Dimensional ◽  
Functional Maturation ◽  
The Matrix ◽  
First Time

Introduction: Heart muscle shows a significant amount of alignment and strategies for cardiac tissue engineering should aim at mimicking such topology. So far, conditions to align cardiomyocytes in three-dimensional (3D) engineered cardiac constructs include static and active mechanical stress and the application of a directional electric field. However, the question of how topological cues from the matrix in which cardiomyocytes are embedded affect their alignment has not been addressed yet. Here, we investigated how cardiomyocytes align and mature in novel 3D gels made out of peptide amphiphiles (PAs) that can be aligned at the nanostructure level. Methods: We seeded HL-1 cardiomyocytes, mouse ES cell-derived cardiomyocytes and human iPS cell-derived cardiomyocytes into PA solutions that were either or not aligned upon gellation. We assessed the alignment of the cardiomyocytes along with their maturation status by observing their structural proteins and the formation of functional syncitiums by connexin 43 expression and the propagation of calcium fluxes. We also tested whether the stiffness of the gel affected the above-mentioned parameters by changing the chemical structure of the PAs. Results: We found that cardiomyocytes aligned along the direction of the alignment of the nanostructures in the gel and that the alignment of the matrix contributed to the functional maturation of the construct. Moreover, we observed a relationship between the stiffness of the gels and the alignment of the cardiomyocytes. Conclusions: This study shows for the first time that the nanostructural features of 3D scaffolds can be exploited to create aligned cardiac constructs.

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