Motion invariant contrast enhancement of optical imaging data in the gradient domain

2016 ◽  
Author(s):  
Philipp Flotho ◽  
Alejandro Romero-Santiago ◽  
Karsten Schwerdtfeger ◽  
Jacek Szczygielski ◽  
Matthias Hulser ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Contrast Enhancement ◽  
Imaging Data ◽  
Gradient Domain

Blind Signal Separation from Optical Imaging Data

2000 ◽  
pp. 91-98
Author(s):  
Ingo Schießl ◽  
Martin Stetter ◽  
John E. W. Mayhew ◽  
Niall McLoughlin ◽  
Jenny S. Lund ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Signal Separation ◽  
Blind Signal Separation ◽  
Imaging Data ◽  
Blind Signal

Analysis of Optical Imaging Data Using Weak Models and ICA

2000 ◽  
pp. 217-233 ◽  
Author(s):  
John Porrill ◽  
James V. Stone ◽  
Jason Berwick ◽  
John Mayhew ◽  
Peter Coffey
Keyword(s):  
Optical Imaging ◽  
Imaging Data

Spatiotemporal analysis of optical imaging data

NeuroImage ◽  
2003 ◽  
Vol 18 (3) ◽  
pp. 610-621 ◽  
Author(s):  
A. Sornborger ◽  
C. Sailstad ◽  
E. Kaplan ◽  
L. Sirovich
Keyword(s):  
Optical Imaging ◽  
Spatiotemporal Analysis ◽  
Imaging Data

scholarly journals In Vivo Tracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells and Ex Vivo Cross-Validation

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Chiara Garrovo ◽  
Natascha Bergamin ◽  
Dave Bates ◽  
Daniela Cesselli ◽  
Antonio Paolo Beltrami ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Optical Imaging ◽  
Adult Stem Cells ◽  
Near Infrared ◽  
Cross Validation ◽  
Ex Vivo ◽  
Imaging Data ◽  
Imaging Results

Stem cells are characterized by the ability to renew themselves and to differentiate into specialized cell types, while stem cell therapy is believed to treat a number of different human diseases through either cell regeneration or paracrine effects. Herein, an in vivo and ex vivo near infrared time domain (NIR TD) optical imaging study was undertaken to evaluate the migratory ability of murine adipose tissue-derived multipotent adult stem cells [mAT-MASC] after intramuscular injection in mice. In vivo NIR TD optical imaging data analysis showed a migration of DiD-labelled mAT-MASC in the leg opposite the injection site, which was confirmed by a fibered confocal microendoscopy system. Ex vivo NIR TD optical imaging results showed a systemic distribution of labelled cells. Considering a potential microenvironmental contamination, a cross-validation study by multimodality approaches was followed: mAT-MASC were isolated from male mice expressing constitutively eGFP, which was detectable using techniques of immunofluorescence and qPCR. Y-chromosome positive cells, injected into wild-type female recipients, were detected by FISH. Cross-validation confirmed the data obtained by in vivo/ex vivo TD optical imaging analysis. In summary, our data demonstrates the usefulness of NIR TD optical imaging in tracking delivered cells, giving insights into the migratory properties of the injected cells.

Furosemide and Mannitol Suppression of Epileptic Activity in the Human Brain

2005 ◽  
Vol 94 (2) ◽  
pp. 907-918 ◽  
Author(s):  
Michael M. Haglund ◽  
Daryl W. Hochman
Keyword(s):  
Electrical Stimulation ◽  
Human Brain ◽  
Optical Imaging ◽  
Neuronal Excitability ◽  
Critical Role ◽  
Intractable Epilepsy ◽  
Mesial Temporal Lobe Epilepsy ◽  
Epileptic Activity ◽  
Imaging Data ◽  
Epileptiform Discharges

Most research on basic mechanisms of epilepsy and the design of new antiepileptic drugs has focused on synaptic transmission or action potential generation. However, a number of laboratory studies have suggested that nonsynaptic mechanisms, such as modulation of electric field interactions via the extracellular space (ECS), might also contribute to neuronal hypersynchrony and epileptogenicity. To date, a role for nonsynaptic modulation of epileptic activity in the human brain has not been investigated. Here we studied the effects of molecules that modulate the volume and water content of the ECS on epileptic activity in patients suffering from neocortical and mesial temporal lobe epilepsy. Electrophysiological and optical imaging data were acquired from the exposed cortices of anesthetized patients undergoing surgical treatment for intractable epilepsy. Patients were given a single intravenous injection containing either 20 mg furosemide (a cation-chloride cotransporter antagonist) or 50 g mannitol (an osmolyte). Furosemide and mannitol both significantly suppressed spontaneous epileptic spikes and electrical stimulation-evoked epileptiform discharges in all subjects, completely blocking all epileptic activity in some patients without suppressing normal electroencephalographic activity. Optical imaging suggested that the spread of electrical stimulation-evoked activity over the cortex was significantly reduced by these treatments, but the magnitude of neuronal activation near the stimulating electrode was not diminished. These results suggest that nonsynaptic mechanisms play a critical role in modulating the epileptogenicity of the human brain. Furosemide and other drugs that modulate the ECS might possess clinically useful antiepileptic properties, while avoiding the side effects associated with the suppression of neuronal excitability.

scholarly journals In Vivo and Ex Vivo Microscopy: Moving Toward the Integration of Optical Imaging Technologies Into Pathology Practice

2018 ◽  
Vol 143 (3) ◽  
pp. 288-298 ◽  
Author(s):  
Wendy A. Wells ◽  
Michael Thrall ◽  
Anastasia Sorokina ◽  
Jeffrey Fine ◽  
Savitri Krishnamurthy ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Ex Vivo ◽  
Imaging Techniques ◽  
Clinical Care ◽  
Imaging Data ◽  
Imaging Technologies ◽  
Optical Images ◽  
Tissue Removal ◽  
Excised Tissue

The traditional surgical pathology assessment requires tissue to be removed from the patient, then processed, sectioned, stained, and interpreted by a pathologist using a light microscope. Today, an array of alternate optical imaging technologies allow tissue to be viewed at high resolution, in real time, without the need for processing, fixation, freezing, or staining. Optical imaging can be done in living patients without tissue removal, termed in vivo microscopy, or also in freshly excised tissue, termed ex vivo microscopy. Both in vivo and ex vivo microscopy have tremendous potential for clinical impact in a wide variety of applications. However, in order for these technologies to enter mainstream clinical care, an expert will be required to assess and interpret the imaging data. The optical images generated from these imaging techniques are often similar to the light microscopic images that pathologists already have expertise in interpreting. Other clinical specialists do not have this same expertise in microscopy, therefore, pathologists are a logical choice to step into the developing role of microscopic imaging expert. Here, we review the emerging technologies of in vivo and ex vivo microscopy in terms of the technical aspects and potential clinical applications. We also discuss why pathologists are essential to the successful clinical adoption of such technologies and the educational resources available to help them step into this emerging role.

Sign in / Sign up

Export Citation Format

Share Document