Robotized High Intensity Focused Ultrasound (HIFU) system for treatment of mobile organs using motion tracking by ultrasound imaging: An in vitro study

2015 ◽  
Author(s):  
Laure-Anais Chanel ◽  
Florent Nageotte ◽  
Jonathan Vappou ◽  
Jianwen Luo ◽  
Loic Cuvillon ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
High Intensity ◽  
Motion Tracking ◽  
Focused Ultrasound ◽  
In Vitro Study ◽  
High Intensity Focused Ultrasound ◽  
Vitro Study

Minimization of treatment time for in vitro 1.1MHz destruction of Pseudomonas aeruginosa biofilms by high-intensity focused ultrasound

Ultrasonics ◽  
2012 ◽  
Vol 52 (5) ◽  
pp. 668-675 ◽  
Author(s):  
Jin Xu ◽  
Timothy A. Bigelow ◽  
Larry J. Halverson ◽  
Jill M. Middendorf ◽  
Ben Rusk
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Intensity ◽  
Focused Ultrasound ◽  
Treatment Time ◽  
High Intensity Focused Ultrasound

scholarly journals A Doxorubicin-Glucuronide Prodrug Released from Nanogels Activated by High-Intensity Focused Ultrasound Liberated β-Glucuronidase

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 536
Author(s):  
Helena C. Besse ◽  
Yinan Chen ◽  
Hans W. Scheeren ◽  
Josbert M. Metselaar ◽  
Twan Lammers ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Anticancer Drugs ◽  
High Intensity ◽  
Focused Ultrasound ◽  
Anticancer Therapy ◽  
High Intensity Focused Ultrasound ◽  
Low Molecular Weight ◽  
Chemotherapeutic Drug ◽  
The Poor

The poor pharmacokinetics and selectivity of low-molecular-weight anticancer drugs contribute to the relatively low effectiveness of chemotherapy treatments. To improve the pharmacokinetics and selectivity of these treatments, the combination of a doxorubicin-glucuronide prodrug (DOX-propGA3) nanogel formulation and the liberation of endogenous β-glucuronidase from cells exposed to high-intensity focused ultrasound (HIFU) were investigated in vitro. First, a DOX-propGA3-polymer was synthesized. Subsequently, DOX-propGA3-nanogels were formed from this polymer dissolved in water using inverse mini-emulsion photopolymerization. In the presence of bovine β-glucuronidase, the DOX-propGA3 in the nanogels was quantitatively converted into the chemotherapeutic drug doxorubicin. Exposure of cells to HIFU efficiently induced liberation of endogenous β-glucuronidase, which in turn converted the prodrug released from the DOX-propGA3-nanogels into doxorubicin. β-glucuronidase liberated from cells exposed to HIFU increased the cytotoxicity of DOX-propGA3-nanogels to a similar extend as bovine β-glucuronidase, whereas in the absence of either bovine β-glucuronidase or β-glucuronidase liberated from cells exposed to HIFU, the DOX-propGA3-nanogels hardly showed cytotoxicity. Overall, DOX-propGA3-nanogels systems might help to further improve the outcome of HIFU-related anticancer therapy.

scholarly journals Localized delivery of compounds into articular cartilage by using high-intensity focused ultrasound

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Heikki J. Nieminen ◽  
Eetu Lampsijärvi ◽  
Gonçalo Barreto ◽  
Mikko A. J. Finnilä ◽  
Ari Salmi ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Articular Cartilage ◽  
High Intensity ◽  
Focused Ultrasound ◽  
Pulse Repetition Frequency ◽  
High Intensity Focused Ultrasound ◽  
Positive Pressure ◽  
Mechanical Index ◽  
Localized Delivery

Abstract Localized delivery of drugs into an osteoarthritic cartilaginous lesion does not yet exist, which limits pharmaceutical management of osteoarthritis (OA). High-intensity focused ultrasound (HIFU) provides a means to actuate matter from a distance in a non-destructive way. In this study, we aimed to deliver methylene blue locally into bovine articular cartilage in vitro. HIFU-treated samples (n = 10) were immersed in a methylene blue (MB) solution during sonication (f = 2.16 MHz, peak-positive-pressure = 3.5 MPa, mechanical index = 1.8, pulse repetition frequency = 3.0 kHz, cycles per burst: 50, duty cycle: 7%). Adjacent control 1 tissue (n = 10) was first pre-treated with HIFU followed by immersion into MB; adjacent control 2 tissue (n = 10) was immersed in MB without ultrasound exposure. The MB content was higher (p < 0.05) in HIFU-treated samples all the way to a depth of 600 µm from AC surface when compared to controls. Chondrocyte viability and RNA expression levels associated with cartilage degeneration were not different in HIFU-treated samples when compared to controls (p > 0.05). To conclude, HIFU delivers molecules into articular cartilage without major short-term concerns about safety. The method is a candidate for a future approach for managing OA.

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