DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells

2012 ◽  
Author(s):  
F. A. Bazley ◽  
A. Pourmorteza ◽  
S. Gupta ◽  
N. Pashai ◽  
C. Kerr ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Cord Injury ◽  
Contusive Spinal Cord Injury

The fate and function of oligodendrocyte progenitor cells after traumatic spinal cord injury

Glia ◽  
2019 ◽  
Vol 68 (2) ◽  
pp. 227-245 ◽  
Author(s):  
Greg J. Duncan ◽  
Sohrab B. Manesh ◽  
Brett J. Hilton ◽  
Peggy Assinck ◽  
Jason R. Plemel ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Traumatic Spinal Cord Injury ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Cord Injury ◽  
And Function

scholarly journals Rapid and Efficient Differentiation of Rodent Neural Stem Cells into Oligodendrocyte Progenitor Cells

2019 ◽  
Vol 41 (1-2) ◽  
pp. 79-93 ◽  
Author(s):  
Shen Li ◽  
Jiao Zheng ◽  
Linlin Chai ◽  
Mengsi Lin ◽  
Ruocheng Zeng ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Progenitor Cells ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Cord Injury

Oligodendrocyte progenitor cells (OPCs) may have beneficial effects in cell replacement therapy of neurodegenerative disease owing to their unique capability to differentiate into myelinogenic oligodendrocytes (OLs) in response to extrinsic signals. Therefore, it is of significance to establish an effective differentiation methodology to generate highly pure OPCs and OLs from some easily accessible stem cell sources. To achieve this goal, in this study, we present a rapid and efficient protocol for oligodendroglial lineage differentiation from mouse neural stem cells (NSCs), rat NSCs, or mouse embryonic stem cell-derived neuroepithelial stem cells. In a defined culture medium containing Smoothened Agonist, basic fibroblast growth factor, and platelet-derived growth factor-AA, OPCs could be generated from the above stem cells over a time course of 4–6 days, achieving a cell purity as high as ∼90%. In particular, these derived OPCs showed high expandability and could further differentiate into myelin basic protein-positive OLs within 3 days or alternatively into glial fibrillary acidic protein-positive astrocytes within 7 days. Furthermore, transplantation of rodent NSC-derived OPCs into injured spinal cord indicated that it is a feasible strategy to treat spinal cord injury. Our results suggest a differentiation strategy for robust production of OPCs and OLs from rodent stem cells, which could provide an abundant OPC source for spinal cord injury.

scholarly journals Oct4‐induced oligodendrocyte progenitor cells enhance functional recovery in spinal cord injury model

2015 ◽  
Vol 34 (23) ◽  
pp. 2971-2983 ◽  
Author(s):  
Jeong Beom Kim ◽  
Hyunah Lee ◽  
Marcos J Araúzo‐Bravo ◽  
Kyujin Hwang ◽  
Donggyu Nam ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Functional Recovery ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Spinal Cord Injury Model ◽  
Injury Model ◽  
Cord Injury

miR-219 overexpressing oligodendrocyte progenitor cells for treating compression spinal cord injury

2021 ◽  
Author(s):  
Bahareh Nazari ◽  
Zeinab Namjoo ◽  
Fatemeh Moradi ◽  
Mansure Kazemi ◽  
Somayeh Ebrahimi-Barough ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Cord Injury

Increase of oligodendrocyte progenitor cells after spinal cord injury

2001 ◽  
Vol 65 (6) ◽  
pp. 500-507 ◽  
Author(s):  
Ken Ishii ◽  
Masahiro Toda ◽  
Yoko Nakai ◽  
Hiroaki Asou ◽  
Masahiko Watanabe ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Cord Injury

scholarly journals Reactive oligodendrocyte progenitor cells (re-)myelinate the regenerating zebrafish spinal cord

Development ◽  
2020 ◽  
Vol 147 (24) ◽  
pp. dev193946 ◽  
Author(s):  
Vasiliki Tsata ◽  
Volker Kroehne ◽  
Daniel Wehner ◽  
Fabian Rost ◽  
Christian Lange ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Progenitor Cells ◽  
Transcriptional Profiling ◽  
Oligodendrocyte Progenitor Cells ◽  
Lesion Site ◽  
Oligodendrocyte Progenitor ◽  
Functional Deficits ◽  
Myelin Sheaths ◽  
Cord Injury ◽  
Global Reduction

ABSTRACTSpinal cord injury (SCI) results in loss of neurons, oligodendrocytes and myelin sheaths, all of which are not efficiently restored. The scarcity of oligodendrocytes in the lesion site impairs re-myelination of spared fibres, which leaves axons denuded, impedes signal transduction and contributes to permanent functional deficits. In contrast to mammals, zebrafish can functionally regenerate the spinal cord. Yet, little is known about oligodendroglial lineage biology and re-myelination capacity after SCI in a regeneration-permissive context. Here, we report that, in adult zebrafish, SCI results in axonal, oligodendrocyte and myelin sheath loss. We find that OPCs, the oligodendrocyte progenitor cells, survive the injury, enter a reactive state, proliferate and differentiate into oligodendrocytes. Concomitantly, the oligodendrocyte population is re-established to pre-injury levels within 2 weeks. Transcriptional profiling revealed that reactive OPCs upregulate the expression of several myelination-related genes. Interestingly, global reduction of axonal tracts and partial re-myelination, relative to pre-injury levels, persist at later stages of regeneration, yet are sufficient for functional recovery. Taken together, these findings imply that, in the zebrafish spinal cord, OPCs replace lost oligodendrocytes and, thus, re-establish myelination during regeneration.

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