Design of multipurpose computational cluster based on ARM single-board computers

2018 ◽  
Author(s):  
Mikhail E. Kryuchkov ◽  
Aleksei V. Orlov ◽  
Grigoriy A. Mazurenko ◽  
Aleksandr B. Vavrenyuk ◽  
Yuriy V. Timofeev
Keyword(s):  
Computational Cluster

High temperature coolant demonstrated for a computational cluster

2016 ◽  
Author(s):  
Egor Druzhinin ◽  
Alexey Shmelev ◽  
Alexander Moskovsky ◽  
Yuri Migal ◽  
Vladimir Mironov ◽  
...  
Keyword(s):  
High Temperature ◽  
Computational Cluster

scholarly journals Computational cluster validation in post-genomic data analysis

2005 ◽  
Vol 21 (15) ◽  
pp. 3201-3212 ◽  
Author(s):  
J. Handl ◽  
J. Knowles ◽  
D. B. Kell
Keyword(s):  
Data Analysis ◽  
Genomic Data ◽  
Cluster Validation ◽  
Computational Cluster ◽  
Genomic Data Analysis

scholarly journals Nanoscale organization of Actin Filaments in the Red Blood Cell Membrane Skeleton

2021 ◽  
Author(s):  
Roberta B. Nowak ◽  
Haleh Alimohamadi ◽  
Kersi Pestonjamasp ◽  
Padmini Rangamani ◽  
Velia M. Fowler
Keyword(s):  
Blood Cell ◽  
Single Molecule ◽  
Red Blood Cell ◽  
Synthetic Data ◽  
Membrane Skeleton ◽  
Membrane Curvature ◽  
Data Sets ◽  
Actin Network ◽  
Computational Cluster ◽  
Planar Network

AbstractRed blood cell (RBC) shape and deformability are supported by a planar network of short actin filament (F-actin) nodes interconnected by long spectrin molecules at the inner surface of the plasma membrane. Spectrin-F-actin network structure underlies quantitative modelling of forces controlling RBC shape, membrane curvature and deformation, yet the nanoscale organization of F-actin nodes in the network in situ is not understood. Here, we examined F-actin distribution in RBCs using fluorescent-phalloidin labeling of F-actin imaged by multiple microscopy modalities. Total internal reflection fluorescence (TIRF) and Zeiss Airyscan confocal microscopy demonstrate that F-actin is concentrated in multiple brightly stained F-actin foci ∼200-300 nm apart interspersed with dimmer F-actin staining regions. Live cell imaging reveals dynamic lateral movements, appearance and disappearance of F-actin foci. Single molecule STORM imaging and computational cluster analysis of experimental and synthetic data sets indicate that individual filaments are non-randomly distributed, with the majority as multiple filaments, and the remainder sparsely distributed as single filaments. These data indicate that F-actin nodes are non-uniformly distributed in the spectrin-F-actin network and necessitate reconsideration of current models of forces accounting for RBC shape and membrane deformability, predicated upon uniform distribution of F-actin nodes and associated proteins across the micron-scale RBC membrane.

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