Isolation and purification of laccase from rice straw fermented with Pleurotus sajor-caju

2010 ◽  
Author(s):  
Nurfariza Bahrin ◽  
Pat M Lee ◽  
Kamsani Ngalib
Keyword(s):  
Rice Straw ◽  
Isolation And Purification ◽  
Pleurotus Sajor Caju

scholarly journals Effects of rice straw fermented with spent Pleurotus sajor-caju mushroom substrates on milking performance in Alpine dairy goats

2022 ◽  
Author(s):  
Geng-Jen Fan ◽  
Mei-Hsing Chen ◽  
Churng-Faung Lee ◽  
Bi Yu ◽  
Tzu-Tai Lee
Keyword(s):  
Rice Straw ◽  
Dairy Goats ◽  
Pleurotus Sajor Caju

scholarly journals Nutritional quality enrichment of rice straw using Pleurotus sajor-caju (Fr.) singer and micro-filamentous fungi

2014 ◽  
Vol 42 (2) ◽  
pp. 333-341 ◽  
Author(s):  
Most Ferdousi Begum ◽  
Abdul Razak Alimon
Keyword(s):  
Mixed Culture ◽  
Filamentous Fungi ◽  
Rice Straw ◽  
Reducing Sugar ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Mineral Contents ◽  
Endoglucanase Activity ◽  
Nutritional Qualities ◽  
Pleurotus Sajor Caju

Solid state fermentation was carried out by Pleurotus sajor-caju, micro-filamentous fungi and culture filtrate of P. sajor-caju with different combinations to improve nutritional qualities of rice straw. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of the fermented rice straw were significantly reduced in mixed culture with filamentous fungi and P. sajor-caju. The cellulose and hemicellulose contents also reduced in similar manner. P. sajor-caju showed strong lignolytic activity resulting cellulose/lignin ratio of 5:1 when rice straw and PKC were supplemented. The protein content increased from 5.86 - 11.12, 12.98 and 13.14%, respectively. The maximum and significant amount of soluble protein 11.84 mg/g and glucosamine 18.50 mg/g was recorded in mixed culture at 30% palm oil kernel cake (PKC) supplementation. The maximum reducing sugar of 18.76 mg/g and endoglucanase activity 11.54 U/ml was recorded in same treatment. The mineral contents K, P, Ca, Mg and Fe were significantly higher in fermented sample while Cu and Zn were non-significant. The antioxidant activity improved by 45% and significantly varied between fermented and unfermented samples. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18039 Bangladesh J. Bot. 42(2): 333-341, 2013 (December)

Immunogold labeling of CMP-KDO synthetase produced by recombinant E. Coli cells

1987 ◽  
Vol 45 ◽  
pp. 924-925
Author(s):  
F. A. Durum ◽  
R. G. Goldman ◽  
T. J. Bolling ◽  
M. F. Miller
Keyword(s):  
Inclusion Bodies ◽  
Large Scale ◽  
Recombinant Dna ◽  
Test System ◽  
Cell Protein ◽  
Gram Negative Bacteria ◽  
Cytoplasmic Inclusions ◽  
Isolation And Purification ◽  
E Coli ◽  
Low Concentrations

CMP-KDO synthetase (CKS) is an enzyme which plays a key role in the synthesis of LPS, an outer membrane component unique to gram negative bacteria. CKS activates KDO to CMP-KDO for incorporation into LPS. The enzyme is normally present in low concentrations (0.02% of total cell protein) which makes it difficult to perform large scale isolation and purification. Recently, the gene for CKS from E. coli was cloned and various recombinant DNA constructs overproducing CKS several thousandfold (unpublished data) were derived. Interestingly, no cytoplasmic inclusions of overproduced CKS were observed by EM (Fig. 1) which is in contrast to other reports of large proteinaceous inclusion bodies in various overproducing recombinant strains. The present immunocytochemical study was undertaken to localize CKS in these cells.Immune labeling conditions were first optimized using a previously described cell-free test system. Briefly, this involves soaking small blocks of polymerized bovine serum albumin in purified CKS antigen and subjecting them to various fixation, embedding and immunochemical conditions.

Effect of thermal treatment on Egyptian rice straw hardboard

1977 ◽  
Vol 27 (1) ◽  
pp. 93-98
Author(s):  
Naim A. Fadl ◽  
Magdi Z. Sefain ◽  
Mohomed Rakha
Keyword(s):  
Thermal Treatment ◽  
Rice Straw

Dairy Farmers Empowerment in Subak Anggabaya Penatih Timur Denpasar Bali Indonesia

2018 ◽  
Vol 9 (05) ◽  
pp. 20218-20223
Author(s):  
I Ketut Arnawa ◽  
I Wayan Runa ◽  
I Made Sukerta ◽  
Putu Sri Astuti ◽  
Dan Luh Kadek Budi Martini
Keyword(s):  
Rice Straw ◽  
Azadirachta Indica ◽  
Nutritional Value ◽  
Organic Fertilizer ◽  
Process Technology ◽  
Dairy Farmers ◽  
Training Activity ◽  
Good Nutrition ◽  
And Training ◽  
Spraying Method

The main purpose for the application of science and technology for livestock group of cow, is to form a group of skilled and professional livestock, as vaccinators, can take advantage of Azadirachta indica leaves a plant insecticide, apply a good cage sanitation, can produce organic fertilizer from feces of cow, can treat hay as feed to good nutritional value. The method used is education and training. Activity results obtained, that 10 percent of members of the group have managed to become vaccinators cadres, able to implement good sanitation stables, feces of cow (Stomoxys calsitrans) can process technology with EM4 into organic fertilizer and can process the rice straw with urea spraying method, to produce feed hay to the value of good nutrition for growing cattle.

MATCHING INGESTIVE BEHAVIOUR OF GOATS TO LOCAL FEED RESOURCES

2013 ◽  
Vol 67 (4) ◽  
Author(s):  
Phan Vũ Hải ◽  
Hồ Trung Thông ◽  
Đàm Văn Tiện
Keyword(s):  
Rice Straw ◽  
Social Facilitation ◽  
Diet Selection ◽  
Feed Resources ◽  
Local Feed Resources ◽  
Ingestive Behaviour

This study was undertaken to find ways of reducing the time taken by goats tobegin to eat an edible feed that they have not previously encountered. Experiment 1demonstrated that the time taken for goats (7-8 months old) to ingest an unfamiliar feed(rice straw) was shorter (4 days) when it was first offered to them in the presence offamiliar positive cues (the odor or flavor of juices extracted from previously eaten,nutritionally beneficial grasses), than if it was offered in the absence of such cues (10 days).In contrast, when the feed was offered in the presence of the odor of parasitised goat feces,the time to first ingestion was extended to 20 days. Experiment 2 showed that when sixmonthold goats were exposed to feeds they had not experienced previously (rice straw orrice bran) they did not ingest these feeds in less than 7 days. However, they commencedingesting these feeds immediately if they had been exposed to them, prior to weaning, inthe presence of their mother or another adult goat. Application of the principles of feedingbehavior, as illustrated by the present studies, to goats in Vietnam may improve theirproduction, especially when diets are changed frequently and include both familiar andunfamiliar materials.Keywords: Behavior; Diet selection; Flavor; Neophobia; Social facilitation; Goat.

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