Effect of mouthwash extracted from Miswak (Salvadora Persica) on periodontal pathogenic bacteria an in-vitro study

2010 ◽  
Author(s):  
Fouad Hussain Al-Bayaty ◽  
Mahmood Ameen Abdulla ◽  
Mohamed Ibrahim Abu Hassan ◽  
Siti Noraini Binti Roslan ◽  
Saba Fouad Hussain ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Vitro Study ◽  
Salvadora Persica

scholarly journals Antimicrobial efficacy of a new tri-antibiotic combination against resistant endodontic pathogens: An in-vitro study

2020 ◽  
Vol 23 (4) ◽  
pp. 8p ◽  
Author(s):  
Prasanna T. Dahake ◽  
Sudhindra M Baliga
Keyword(s):  
Root Canal ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
In Vitro Study ◽  
Vitro Study ◽  
New Combination ◽  
Antimicrobial Efficacy ◽  
E Coli ◽  
Background Removal

Background: Removal of all the pathogenic bacteria from the root canal system is of prime importance for the success of endodontic therapy. Objective: The study aimed to determine the antimicrobial efficacy of three antibiotics and their new combination against selected endodontic pathogens. Methods: In this in-vitro study, we used bacterial strains associated with the refractory endodontic condition and determined MIC and MBC of Clindamycin (C), Metronidazole (M), Doxycycline (D) as well as their combination CMD. We cultured Candida Albicans, Pseudomonas Aeruginosa, Escherichia Coli, Enterococcus Faecalis, Streptococcus Mutans, Bacillus Subtilis subsp. spizizenii, Actinomyces Actinomycetemcomitans on selective culture media. We analyzed the data using paired 't' test, one-way ANOVA, and Tuckey's HSD post hoc test. Results: Clindamycin inhibited the growth of C. Albicans (90%) and S. Mutans (90%) significantly and P. Aeruginosa, E. Coli, E. Faecalis, B. Subtilis, and A. Actinomycetemcomitans were resistant to it. Metronidazole did not inhibit any of the bacteria. Doxycycline inhibited C. Albicans (90%), P. Aeruginosa (90%), and S. Mutans (90%) significantly while E. Coli, E. Faecalis, B. Subtilis, and A. Actinomycetemcomitans were resistant to it. The combination of CMD inhibited all the microbes significantly. However, at bactericidal concentrations of CMD, E. Faecalis (p = 0.024), B. Subtilis (p = 0.021) and A. Actinomycetemcomitans (p = 0.041) were eliminated significantly, while C. Albicans (p = 0.164), P. Aeruginosa (p = 0.489), E. Coli (p = 0.106) and S. Mutans (p = 0.121) showed resistance. Conclusion: Combination CMD can be used against resistant endodontic pathogens to achieve predictable endodontic results.KEYWORDSAntimicrobial agents; Clindamycin; Doxycycline; Metronidazole; Root canal therapy.    

scholarly journals Inhibitory effect of Salvadora persica extract (Miswak) on collagen degradation in demineralized dentin: In vitro study

2021 ◽  
Vol 16 (1) ◽  
pp. 208-213
Author(s):  
Sahar Khunkar ◽  
Ilnaz Hariri ◽  
Ehab Alsayed ◽  
Amal Linjawi ◽  
Sawsan Khunkar ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Inhibitory Effect ◽  
Collagen Degradation ◽  
Vitro Study ◽  
Salvadora Persica ◽  
Demineralized Dentin

In Vitro Study on Commercial Organic Acid Activate WD (WD) Against Four Pathogenic Bacteria

2012 ◽  
Vol 19 (4) ◽  
pp. 62-65
Author(s):  
Jiang Guo-zheng ◽  
Li Ji-chang ◽  
Han Zhen-xing ◽  
Liu Ting ◽  
Wang Yuan ◽  
...  
Keyword(s):  
Organic Acid ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Antibacterial Activity of Haslea ostrearia Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)

Omni-Akuatika ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 30
Author(s):  
Ikfa Permatasari ◽  
Fiddy Semba Prasetiya ◽  
Sulastri Arsad ◽  
Mochamad Untung Kurnia Agung ◽  
Jean-Luc Mouget
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
In Vitro Study ◽  
Water Soluble ◽  
Vitro Study ◽  
Inhibitory Zone ◽  
Haslea Ostrearia

Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatic

A Comparative In Vivo and In Vitro Study on the Effectiveness of Homoeopathic Medicines on Aquarium Zebrafish Infected with Vibrio parahaemolyticus

2020 ◽  
Vol 33 (04) ◽  
pp. 263-271
Author(s):  
A.S. Aiswarya ◽  
K.R. Reshmy ◽  
C.V. Chandraja ◽  
P.R. Sisir
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Serial Dilution ◽  
Vitro Study ◽  
Resistant Bacteria ◽  
Deep Acting ◽  
Agar Media

Abstract Background Human infections associated with Vibrio parahaemolyticus rank high among the pathogenic bacteria associated with fishes which causes septicaemia and gastroenteritis in humans. Humans acquire such infections by handling of infected fishes. Therefore, rather than curing the diseases in humans, it can be prevented in fishes by homoeopathic medicines. Objectives The main objective of the study is to show the effectiveness of homoeopathic medicines in curing aquarium zebrafish infected with Vibrio parahaemolyticus. Materials and Methods Fifteen pairs of healthy aquarium zebrafish were chosen for the study. They were inoculated with Vibrio parahaemolyticus after the acclimatisation period of 1 month. The signs were noted down in chronological order. homoeopathic medicines Sulphur 200, Arsenicum album 200 and Pyrogenium 200 was administered based on the totality obtained. An in vitro study was also done by the serial dilution of water from each tank and also by the serial dilution of the fish gut which was plated in thiosulphate-citrate-bile salts-sucrose (TCBS) agar media. A growth sensitivity study to show the growth inhibition was done on Muller Hinton agar media. Results Although the incubation period of vibriosis was 4 days, the condition was completely cured within 10 days of administration of homoeopathic medicines. But in the case of tetracycline, the signs disappeared after 15 days. Sulphur being deep acting remedy showed cure in fishes as well as in the water. Arsenicum album showed therapeutic as well as prophylactic characters. Conclusion From the study, it is proved that the Sulphur 200 was the most effective remedy. Homoeopathic medicines do not produce any resistant bacteria but improves the immunity of the living organism. Sulphur, the deep acting remedy ensures 100% cure, i.e., both the water and the fishes are cured of Vibrio parahaemolyticus.

In vitro Study of the Antimicrobial Effects of Phenolic Extract of the Salvadora persica (Miswak) on the Growth of Certain Microorganisms Responsible for Oral Infections

2016 ◽  
Vol 12 (1) ◽  
pp. 58-73 ◽  
Author(s):  
Ouiza Ait Chaban ◽  
Djamel Ait Saada ◽  
Ahmed Mohamed Al ◽  
Ghalem Selselet-A ◽  
Kaddour Bouderoua ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Salvadora Persica ◽  
Antimicrobial Effects ◽  
Oral Infections ◽  
Phenolic Extract
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