Comparative Analysis of Sequence Variability of Dengue Virus Serotypes Using Second Generation Wavelets

2017 ◽  
Author(s):  
Shiwani Saini ◽  
Lillie Dewan
Keyword(s):  
Comparative Analysis ◽  
Dengue Virus ◽  
Second Generation ◽  
Sequence Variability ◽  
Dengue Virus Serotypes ◽  
Second Generation Wavelets

scholarly journals Two Complex, Adenovirus-Based Vaccines That Together Induce Immune Responses to All Four Dengue Virus Serotypes

2006 ◽  
Vol 14 (2) ◽  
pp. 182-189 ◽  
Author(s):  
David H. Holman ◽  
Danher Wang ◽  
Kanakatte Raviprakash ◽  
Nicholas U. Raja ◽  
Min Luo ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dengue Virus ◽  
Vaccine Development ◽  
De Novo ◽  
Natural Infection ◽  
Hemorrhagic Fever ◽  
Virus Infections ◽  
Dengue Vaccine ◽  
Membrane Antigens ◽  
Dengue Virus Serotypes

ABSTRACT Dengue virus infections can cause hemorrhagic fever, shock, encephalitis, and even death. Worldwide, approximately 2.5 billion people live in dengue-infested regions with about 100 million new cases each year, although many of these infections are believed to be silent. There are four antigenically distinct serotypes of dengue virus; thus, immunity from one serotype will not cross-protect from infection with the other three. The difficulties that hamper vaccine development include requirements of the natural conformation of the envelope glycoprotein to induce neutralizing immune responses and the necessity of presenting antigens of all four serotypes. Currently, the only way to meet these requirements is to use a mixture of four serotypes of live attenuated dengue viruses, but safety remains a major problem. In this study, we have developed the basis for a tetravalent dengue vaccine using a novel complex adenovirus platform that is capable of expressing multiple antigens de novo. This dengue vaccine is constructed as a pair of vectors that each expresses the premembrane and envelope genes of two different dengue virus serotypes. Upon vaccination, the vaccine expressed high levels of the dengue virus antigens in cells to mimic a natural infection and induced both humoral and cellular immune responses against multiple serotypes of dengue virus in an animal model. Further analyses show the humoral responses were indeed neutralizing against all four serotypes. Our studies demonstrate the concept of mimicking infections to induce immune responses by synthesizing dengue virus membrane antigens de novo and the feasibility of developing an effective tetravalent dengue vaccine by vector-mediated expression of glycoproteins of the four serotypes.

scholarly journals Vector competence of Malaysian Aedes albopictus with and without Wolbachia to four dengue virus serotypes

2017 ◽  
Vol 22 (9) ◽  
pp. 1154-1165 ◽  
Author(s):  
Sylvia Joanne ◽  
Indra Vythilingam ◽  
Boon-Teong Teoh ◽  
Cherng-Shii Leong ◽  
Kim-Kee Tan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
Vector Competence ◽  
Dengue Virus Serotypes

Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay

2021 ◽  
Author(s):  
Kundan Tandel ◽  
Mahadevan Kumar ◽  
G.S. Bhalla ◽  
S.P.S. Shergill ◽  
Vijaya Swarnim ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Dengue Virus Serotypes

Co-circulation of dengue virus serotypes with chikungunya virus in Madhya Pradesh, central India

2014 ◽  
Vol 3 (1) ◽  
pp. 36 ◽  
Author(s):  
Neeru Singh ◽  
PradipV Barde ◽  
MohanK Shukla ◽  
PraveenK Bharti ◽  
BhupeshK Kori ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Chikungunya Virus ◽  
Central India ◽  
Madhya Pradesh ◽  
Dengue Virus Serotypes
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