Simultaneous assessing the intracellular potassium and calcium concentrations noninvasively in vivo with high resolution fluorescence imaging

2011 ◽  
Author(s):  
Hugang Ren ◽  
Zhongchi Luo ◽  
S. Elinav ◽  
Yingtian Pan ◽  
R. Fine ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Intracellular Potassium

In Vivo High-resolution Ratiometric Fluorescence Imaging of Inflammation Using NIR-II Nanoprobes with 1550 nm Emission

Nano Letters ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 2418-2427 ◽  
Author(s):  
Shangfeng Wang ◽  
Lu Liu ◽  
Yong Fan ◽  
Ahmed Mohamed El-Toni ◽  
Mansour Saleh Alhoshan ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Ratiometric Fluorescence

Optical fibers for high-resolution in vivo microendoscopic fluorescence imaging

2013 ◽  
Vol 19 (6) ◽  
pp. 760-771 ◽  
Author(s):  
Gyungseok Oh ◽  
Euiheon Chung ◽  
Seok H. Yun
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Optical Fibers

scholarly journals In vivo fluorescence imaging with high-resolution microlenses

2009 ◽  
Vol 6 (7) ◽  
pp. 511-512 ◽  
Author(s):  
Robert P J Barretto ◽  
Bernhard Messerschmidt ◽  
Mark J Schnitzer
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging

scholarly journals Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae

2021 ◽  
Author(s):  
Fumiaki Nin ◽  
Samuel Choi ◽  
Takeru Ota ◽  
Zhang Qi ◽  
Hiroshi Hibino
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Guinea Pig ◽  
Hair Cells ◽  
Sensory Epithelium ◽  
Outer Hair Cells ◽  
Acquisition Time ◽  
Total Acquisition Time ◽  
Sd Oct

AbstractSound evokes sub-nanoscale vibration within the sensory epithelium. The epithelium contains not only immotile cells but also contractile outer hair cells (OHCs) that actively shrink and elongate synchronously with the sound. However, the in vivo motion of OHCs has remained undetermined. The aim of this work is to perform high-resolution and -accuracy vibrometry in live guinea pigs with an SC-introduced spectral-domain optical coherence tomography system (SD-OCT). In this study, to reveal the effective contribution of SC source in the recording of the low reflective materials with the short total acquisition time, we compare the performances of the SC-introduced SD-OCT (SCSD-OCT) to that of the conventional SD-OCT. As inanimate comparison objects, we record a mirror, a piezo actuator, and glass windows. For the measurements in biological materials, we use in/ex vivo guinea pig cochleae. Our study achieved the optimization of a SD-OCT system for high-resolution in vivo vibrometry in the cochlear sensory epithelium, termed the organ of Corti, in mammalian cochlea. By introducing a supercontinuum (SC) light source and reducing the total acquisition time, we improve the axial resolution and overcome the difficulty in recording the low reflective material in the presence of biological noise. The high power of the SC source enables the system to achieve a spatial resolution of 1.72 ± 0.00 μm on a mirror and reducing the total acquisition time contributes to the high spatial accuracy of sub-nanoscale vibrometry. Our findings reveal the vibrations at the apical/basal region of OHCs and the extracellular matrix, basilar membrane.

scholarly journals Iron accumulation in the oculomotor nerve of the progressive supranuclear palsy brain

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hansol Lee ◽  
Myung Jun Lee ◽  
Eun-Joo Kim ◽  
Gi Yeong Huh ◽  
Jae-Hyeok Lee ◽  
...  
Keyword(s):  
High Resolution ◽  
Progressive Supranuclear Palsy ◽  
Oculomotor Nerve ◽  
Iron Accumulation ◽  
Blue Staining ◽  
Normal Brain ◽  
Myelinated Fiber ◽  
Healthy Controls ◽  
Icp Ms

AbstractAbnormal iron accumulation around the substantia nigra (SN) is a diagnostic indicator of Parkinsonism. This study aimed to identify iron-related microarchitectural changes around the SN of brains with progressive supranuclear palsy (PSP) via postmortem validations and in vivo magnetic resonance imaging (MRI). 7 T high-resolution MRI was applied to two postmortem brain tissues, from one normal brain and one PSP brain. Histopathological examinations were performed to demonstrate the molecular origin of the high-resolution postmortem MRI findings, by using ferric iron staining, myelin staining, and two-dimensional laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) imaging. In vivo iron-related MRI was performed on five healthy controls, five patients with Parkinson’s disease (PD), and five patients with PSP. In the postmortem examination, excessive iron deposition along the myelinated fiber at the anterior SN and third cranial nerve (oculomotor nerve) fascicles of the PSP brain was verified by LA-ICP-MS. This region corresponded to those with high R2* values and positive susceptibility from quantitative susceptibility mapping (QSM), but was less sensitive in Perls’ Prussian blue staining. In in vivo susceptibility-weighted imaging, hypointense pixels were observed in the region between the SN and red nucleus (RN) in patients with PSP, but not in healthy controls and patients with PD. R2* and QSM values of such region were significantly higher in patients with PSP compared to those in healthy controls and patients with PD as well (vs. healthy control: p = 0.008; vs. PD: p = 0.008). Thus, excessive iron accumulation along the myelinated fibers at the anterior SN and oculomotor nerve fascicles may be a pathological characteristic and crucial MR biomarker in a brain with PSP.

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