Coagulation-Fenton Research on Oxidation Process Ion Exchange Resin Regenerating Wastewater

Author(s):  
Che Chunbo
2017 ◽  
Vol 12 (1) ◽  
pp. 8
Author(s):  
Muhammad Nur ◽  
Edi Munawar ◽  
Mariana Mariana

Abstract Study of reducing the content of ammonia, carbon dioxide and organic contaminants in wastewater process condensate using a combination of technologies of water Jet Stripping Bubble Colum, Advance Oxidation Process and Ion Exchange Resin have been done. The aims of this research is to look the effect of hydraulic resident time in Jet Stripping Bubble Colum and the amount of the addition of Ozon/H2O2 to reduction the content of carbon dioxide and organic contaminants as well as ammonia removal with ion exchange resin on the process condensate so that can be reuse as boiler feed water in ammonia plant of PT. PIM. This research was conducted using a combination Air Stripping Jet Bubble Colum, Advance Oxidation Process and Ion Exchange Resin which equipped with water and air flows adjustment. The water flow enter the column, pressure of the Colum and diameter of nozzle was maintained constantnts during experiment, while the air flow rate enter to the column, H2O2/O3 concentrations and the hydraulic retention time was vary  respectively. The results showed that the water Jet Stripping Bubble Colum at 50 Psi air pressure capable of achieving CO2 lowers 87.60% and ammonia to 65.31%. AOP technology capable of lowering the content of organic contaminant to 80.71% with the addition of hydrogen peroxide 8,000 ppm with a residence time 180 minutes. While the ammonia remaining in the water the process of condensate action exchange resin using eliminated with a reduction of ammonia reached 98.57%, so that the recovery process of water condensate technology uses a combination of Air Stripping Jet Bubble Colum, Advance Hydrocarbon processes (AOP) and Ion Exchange Resins already meets the requirements of viable reuses boiler feed water. The ammonia reduction was determined by using ASTM-D 1426 method, the carbon dioxide by using ASTM-512 method, and the organic contaminants by using titrimetric method (SII-0071). Keyword   :  Air Stripping Jet Bubble Colum, Advance Oxidation Process (AOP), Ammonia Removal, Carbon Dioxide Removal.


2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.


1977 ◽  
Vol 49 (6) ◽  
pp. 764-766 ◽  
Author(s):  
Toshihiko. Hanai ◽  
Harold F. Walton

RSC Advances ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 4478-4488
Author(s):  
Sivaprakasam Anbazhagan ◽  
Venugopal Thiruvengadam ◽  
Anandhakumar Sukeri

We have demonstrated a high Pb2+ removal efficiency (73.45%) from wastewater using a Prosopis juliflora-seed-modified Amberlite IRA-400 Cl− ion-exchange resin (SMA resin).


2019 ◽  
Vol 138 ◽  
pp. 681-692 ◽  
Author(s):  
Marina G. Holyavka ◽  
Maxim S. Kondratyev ◽  
Anatoly N. Lukin ◽  
Boris L. Agapov ◽  
Valery G. Artyukhov

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