Observers for the biotechnological processes with unknown kinetics. Application to wastewater treatment

2002 ◽  
Author(s):  
O. Bernard ◽  
Z. Hadj-Sadok ◽  
J.-L. Gouze
Keyword(s):  
Wastewater Treatment ◽  
Biotechnological Processes

Biotechnological perspectives of microorganisms isolated from the Polar Regions

2018 ◽  
Vol 39 (3) ◽  
pp. 137
Author(s):  
Viktoria Shcherbakova ◽  
Olga Troshina
Keyword(s):  
Wastewater Treatment ◽  
Low Temperature ◽  
Liquid Water ◽  
Polar Regions ◽  
Biotechnological Applications ◽  
Cold Adapted ◽  
Culture Independent ◽  
Biotechnological Processes ◽  
Improved Methods ◽  
Culture Dependent

Polar permanently frozen grounds cover more than 20% of the earth's surface, and about 60% of the Russian territories are permafrost. In the permafrost environments, the combination of low temperature and poor availability of liquid water make these habitats extremely inhospitable for life. To date, both culture-dependent and culture-independent methods have shown that permafrost is a habitat for microorganisms of all three domains: Bacteria, Archaea and Eukarya. An overview of applying psychrophilic and psychrotolerant bacteria and archaea isolated from Arctic and Antarctic permafrost ecosystems in biotechnological processes of wastewater treatment, production of cold-adapted enzymes, etc. is discussed here. The study of existing collections of microorganisms isolated from permanently cold habitats, improved methods of sampling and enrichment will increase the potential biotechnological applications of permafrost bacteria and archaea producing unique biomolecules.

scholarly journals From Genomes to Phenotypes: Traitar, the Microbial Trait Analyzer

mSystems ◽  
2016 ◽  
Vol 1 (6) ◽  
Author(s):  
Aaron Weimann ◽  
Kyra Mooren ◽  
Jeremy Frank ◽  
Phillip B. Pope ◽  
Andreas Bremges ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Human Health ◽  
Bacterial Communities ◽  
Genome Sequences ◽  
Bacterial Genomes ◽  
Human Gut ◽  
Polluted Soils ◽  
Lab Experiments ◽  
Wet Lab ◽  
Biotechnological Processes

ABSTRACT Bacteria are ubiquitous in our ecosystem and have a major impact on human health, e.g., by supporting digestion in the human gut. Bacterial communities can also aid in biotechnological processes such as wastewater treatment or decontamination of polluted soils. Diverse bacteria contribute with their unique capabilities to the functioning of such ecosystems, but lab experiments to investigate those capabilities are labor-intensive. Major advances in sequencing techniques open up the opportunity to study bacteria by their genome sequences. For this purpose, we have developed Traitar, software that predicts traits of bacteria on the basis of their genomes. It is applicable to studies with tens or hundreds of bacterial genomes. Traitar may help researchers in microbiology to pinpoint the traits of interest, reducing the amount of wet lab work required. The number of sequenced genomes is growing exponentially, profoundly shifting the bottleneck from data generation to genome interpretation. Traits are often used to characterize and distinguish bacteria and are likely a driving factor in microbial community composition, yet little is known about the traits of most microbes. We describe Traitar, the microbial trait analyzer, which is a fully automated software package for deriving phenotypes from a genome sequence. Traitar provides phenotype classifiers to predict 67 traits related to the use of various substrates as carbon and energy sources, oxygen requirement, morphology, antibiotic susceptibility, proteolysis, and enzymatic activities. Furthermore, it suggests protein families associated with the presence of particular phenotypes. Our method uses L1-regularized L2-loss support vector machines for phenotype assignments based on phyletic patterns of protein families and their evolutionary histories across a diverse set of microbial species. We demonstrate reliable phenotype assignment for Traitar to bacterial genomes from 572 species of eight phyla, also based on incomplete single-cell genomes and simulated draft genomes. We also showcase its application in metagenomics by verifying and complementing a manual metabolic reconstruction of two novel Clostridiales species based on draft genomes recovered from commercial biogas reactors. Traitar is available at https://github.com/hzi-bifo/traitar . IMPORTANCE Bacteria are ubiquitous in our ecosystem and have a major impact on human health, e.g., by supporting digestion in the human gut. Bacterial communities can also aid in biotechnological processes such as wastewater treatment or decontamination of polluted soils. Diverse bacteria contribute with their unique capabilities to the functioning of such ecosystems, but lab experiments to investigate those capabilities are labor-intensive. Major advances in sequencing techniques open up the opportunity to study bacteria by their genome sequences. For this purpose, we have developed Traitar, software that predicts traits of bacteria on the basis of their genomes. It is applicable to studies with tens or hundreds of bacterial genomes. Traitar may help researchers in microbiology to pinpoint the traits of interest, reducing the amount of wet lab work required.

scholarly journals Filamentous Fungi as Promising Agents for the Biodegradation of Biosolids Compounds

2019 ◽  
Vol 8 (2) ◽  
pp. 35-51
Author(s):  
Laiza Rosa Naves ◽  
Lucas Leonardo Da Silva ◽  
Elida Lucia Da Cunha ◽  
Verediana Fiorentin Rosa De Almeida ◽  
Antônio Sérgio Ferreira De Sá ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Filamentous Fungi ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Carboxymethyl Cellulase ◽  
Biotechnological Processes ◽  
Potential Use

Wastewater Treatment Plant (WWTP) generate pasty wastes, known biosolids, which can be toxic and recalcitrant, motivating studies aiming at their degradation. Filamentous fungi were investigated to degradation of biosolids from a WWTP in the Goiânia, Goiás, Brazil. All grew in the presence of biosolids, being inhibited by increase in concentration, except SXS629, which increased proportionally to the concentration. All grew in the middle with biosolids at the original pH (10.5), although the correction (6.8) provided higher growth. Except SXS90, the others (SXS37, SXS615 and SXS628) degraded the biosolid, growing in medium containing biosolids as the only source of carbon; highlighting SXS628, whose growth in the biosolids exceeded the control. All evaluated isolates synthesize at least two prospected enzymes, especially SXS630 and SXS634, which synthesize all (carboxymethyl cellulase, tannase, polyphenoloxidase). This shows the potential use of these isolates (combined or not) in biotechnological processes aiming at the degradation of biosolids, especially SXS37.

Application of Thermostable Lipolytic Bacterial Enzymes for Modern Biotechnological Processes: Review

2018 ◽  
Vol 18 (6) ◽  
pp. 61-73 ◽  
Author(s):  
Yu. V. Samoilova ◽  
K. N. Sorokina ◽  
A. V. Piligaev ◽  
V. N. Parmon
Keyword(s):  
Wastewater Treatment ◽  
Substrate Specificity ◽  
Organic Solvents ◽  
Food Industry ◽  
Review Paper ◽  
Chemical Compounds ◽  
Functional Stability ◽  
Bacterial Enzymes ◽  
Biotechnological Processes

In the review paper, the modern investigations on the application of thermostable lipolytic bacterial enzymes for biotechnology are discussed, the properties of these enzymes discussed including their activity and functional stability at various temperatures, pH in organic solvents, as well as the substrate specificity and activity in the presence of various chemical compounds. The paper contains data on the development of recombinant producers of lipolytic bacterial enzymes and on approaches to improving their productivity. The application of the bacterial lipases for biotechnological processes of synthesis of biofuel, various chemicals and detergents, for food industry and wastewater treatment is considered.

Micropollutants: a bottleneck in sustainable wastewater treatment

1997 ◽  
Vol 35 (10) ◽  
pp. 13-26 ◽  
Author(s):  
I. Janssens ◽  
T. Tanghe ◽  
W. Verstraete
Keyword(s):  
Wastewater Treatment ◽  
Water Treatment ◽  
Large Scale ◽  
Threshold Values ◽  
Reclaimed Wastewater ◽  
Clear Cut ◽  
Drinking Water Production ◽  
Microbial Associations ◽  
Biotechnological Processes ◽  
Sustainable Wastewater Treatment

Every year a variety of xenobiotic chemicals as pesticides, dyes,… are introduced on a very large scale. The majority of these compounds have a rather poor biodegradability. Hence, fresh water resources become more and more contaminated with micro-quantities of these man-made pollutants. Moreover, some of these obscure pollutants may have the undesirable capability of having oestrogenic activity on various high forms of life. This paper deals with the problems of micropollutants in drinking water production and wastewater treatment. The biocatalytic properties of microorganisms can be limiting for several reasons such as lower threshold values for metabolism, insufficient free energy change or inadequate metabolic knowledge base in the microbial cell or community. The limiting biodegradative capacity of natural microbial associations necessitates the development of more integrated water treatment and management. Research is necessary on two levels i.e. the search for biotechnological processes able to remove such chemicals through engineering of pathways and microbial associations, and the need for reliable biosensors able to generate information on residual microorganics. Trends such as improved biocatalysis and accurate process control are of major significance. However, a clear-cut scientific and political endorsement of the necessity to use reclaimed wastewater is of prime importance to evolve towards sustainable water treatment and management.

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