scholarly journals Homography based parallel volume intersection: toward real-time volume reconstruction using active cameras

Author(s):  
T. Wada ◽  
Xiaojun Wu ◽  
S. Tokai ◽  
T. Matsuyama
2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Prashant Kumar ◽  
Prakash Joshi ◽  
Jigmi Basumatary ◽  
Partha Pratim Mondal

AbstractOptical imaging is paramount for disease diagnosis and to access its progression over time. The proposed optical flow imaging (VFC/iLIFE) is a powerful technique that adds new capabilities (3D volume visualization, organelle-level resolution, and multi-organelle screening) to the existing system. Unlike state-of-the-art point-illumination-based biomedical imaging techniques, the sheet-based VFC technique is capable of single-shot sectional visualization, high throughput interrogation, real-time parameter estimation, and instant volume reconstruction with organelle-level resolution of live specimens. The specimen flow system was realized on a multichannel (Y-type) microfluidic chip that enables visualization of organelle distribution in several cells in-parallel at a relatively high flow-rate (2000 nl/min). The calibration of VFC system requires the study of point emitters (fluorescent beads) at physiologically relevant flow-rates (500–2000 nl/min) for determining flow-induced optical aberration in the system point spread function (PSF). Subsequently, the recorded raw images and volumes were computationally deconvolved with flow-variant PSF to reconstruct the cell volume. High throughput investigation of the mitochondrial network in HeLa cancer cell was carried out at sub-cellular resolution in real-time and critical parameters (mitochondria count and size distribution, morphology, entropy, and cell strain statistics) were determined on-the-go. These parameters determine the physiological state of cells, and the changes over-time, revealing the metastatic progression of diseases. Overall, the developed VFC system enables real-time monitoring of sub-cellular organelle organization at a high-throughput with high-content capacity.


2010 ◽  
Author(s):  
Philipp Stolka ◽  
Hyun-jae Kang ◽  
Emad Boctor

Ultrasound is a very useful and convenient modality in medical imaging, due to its relatively small footprint, high resolution, negligible adverse effects, and real-time imaging capability – in spite of its well-known artifacts. However, it is fairly difficult to fully benefit from its resolution and speed in advanced imaging research because of the closed nature of most ultrasound systems.We propose a modular and extensible open-source toolkit MUSiiC S/W for rapid development and system setup for ultrasound research. Aimed at algorithm and experimental system builders as well as at clinical research, it allows standardized access to various types of ultrasound-derived real-time data streams, incorporates various tracking facilities, volume reconstruction, and connections to external software tools. By providing well-defined open standard interfaces between those building blocks, we aim at easy extensibility for third-party researchers.


2007 ◽  
Vol 24 (9) ◽  
pp. 967-974 ◽  
Author(s):  
Osama I.I. Soliman ◽  
Boudewijn J. Krenning ◽  
Marcel L. Geleijnse ◽  
Attila Nemes ◽  
Robert-Jan van Geuns ◽  
...  

1979 ◽  
Vol 44 ◽  
pp. 41-47
Author(s):  
Donald A. Landman

This paper describes some recent results of our quiescent prominence spectrometry program at the Mees Solar Observatory on Haleakala. The observations were made with the 25 cm coronagraph/coudé spectrograph system using a silicon vidicon detector. This detector consists of 500 contiguous channels covering approximately 6 or 80 Å, depending on the grating used. The instrument is interfaced to the Observatory’s PDP 11/45 computer system, and has the important advantages of wide spectral response, linearity and signal-averaging with real-time display. Its principal drawback is the relatively small target size. For the present work, the aperture was about 3″ × 5″. Absolute intensity calibrations were made by measuring quiet regions near sun center.


Sign in / Sign up

Export Citation Format

Share Document