Rapid enumeration of bacterial cells in drinking water using a microfluidic device

2006 ◽  
Author(s):  
Nobuyasu Yamaguchi ◽  
Chieko Sakamoto ◽  
Masumi Yamada ◽  
Hiroyasu Nagase ◽  
Minoru Seki ◽  
...  
Keyword(s):  
Drinking Water ◽  
Microfluidic Device ◽  
Bacterial Cells ◽  
Rapid Enumeration

scholarly journals Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

2020 ◽  
Author(s):  
Benjamin Krafft ◽  
Anna Tycova ◽  
Raphael D. Urban ◽  
Christian Dusny ◽  
Detlev Belder
Keyword(s):  
Drinking Water ◽  
Microfluidic Device

scholarly journals Protozoan Bacterivory and Escherichia coli Survival in Drinking Water Distribution Systems

1998 ◽  
Vol 64 (1) ◽  
pp. 197-202 ◽  
Author(s):  
I. Sibille ◽  
T. Sime-Ngando ◽  
L. Mathieu ◽  
J. C. Block
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Microbial Communities ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Trophic Relationships ◽  
Bacterial Cells ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

ABSTRACT The development of bacterial communities in drinking water distribution systems leads to a food chain which supports the growth of macroorganisms incompatible with water quality requirements and esthetics. Nevertheless, very few studies have examined the microbial communities in drinking water distribution systems and their trophic relationships. This study was done to quantify the microbial communities (especially bacteria and protozoa) and obtain direct and indirect proof of protozoan feeding on bacteria in two distribution networks, one of GAC water (i.e., water filtered on granular activated carbon) and the other of nanofiltered water. The nanofiltered water-supplied network contained no organisms larger than bacteria, either in the water phase (on average, 5 × 107bacterial cells liter−1) or in the biofilm (on average, 7 × 106 bacterial cells cm−2). No protozoa were detected in the whole nanofiltered water-supplied network (water plus biofilm). In contrast, the GAC water-supplied network contained bacteria (on average, 3 × 108 cells liter−1 in water and 4 × 107 cells cm−2 in biofilm) and protozoa (on average, 105cells liter−1 in water and 103 cells cm−2 in biofilm). The water contained mostly flagellates (93%), ciliates (1.8%), thecamoebae (1.6%), and naked amoebae (1.1%). The biofilm had only ciliates (52%) and thecamoebae (48%). Only the ciliates at the solid-liquid interface of the GAC water-supplied network had a measurable grazing activity in laboratory test (estimated at 2 bacteria per ciliate per h). Protozoan ingestion of bacteria was indirectly shown by adding Escherichia colito the experimental distribution systems. Unexpectedly, E. coli was lost from the GAC water-supplied network more rapidly than from the nanofiltered water-supplied network, perhaps because of the grazing activity of protozoa in GAC water but not in nanofiltered water. Thus, the GAC water-supplied network contained a functional ecosystem with well-established and structured microbial communities, while the nanofiltered water-supplied system did not. The presence of protozoa in drinking water distribution systems must not be neglected because these populations may regulate the autochthonous and allochthonous bacterial populations.

scholarly journals Microfluidic Device for Automated Synchronization of Bacterial Cells

2012 ◽  
Vol 84 (20) ◽  
pp. 8571-8578 ◽  
Author(s):  
Seth M. Madren ◽  
Michelle D. Hoffman ◽  
Pamela J. B. Brown ◽  
David T. Kysela ◽  
Yves V. Brun ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Bacterial Cells

Rapid quantification of bacterial cells in potable water using a simplified microfluidic device

2007 ◽  
Vol 68 (3) ◽  
pp. 643-647 ◽  
Author(s):  
Chieko Sakamoto ◽  
Nobuyasu Yamaguchi ◽  
Masumi Yamada ◽  
Hiroyasu Nagase ◽  
Minoru Seki ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Potable Water ◽  
Bacterial Cells ◽  
Rapid Quantification

scholarly journals Statistical optimization of the lysis agents for gram-negative bacterial cells in a microfluidic device

2006 ◽  
Vol 11 (6) ◽  
pp. 557-557
Author(s):  
Young-Bum Kim ◽  
Ji-Ho Park ◽  
Woo-Jin Chang ◽  
Yoon-Mo Koo ◽  
Eun-Ki Kim ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Statistical Optimization ◽  
Bacterial Cells ◽  
Gram Negative

scholarly journals Rapid, Semiautomated Quantification of Bacterial Cells in Freshwater by Using a Microfluidic Device for On-Chip Staining and Counting

2010 ◽  
Vol 77 (4) ◽  
pp. 1536-1539 ◽  
Author(s):  
Nobuyasu Yamaguchi ◽  
Masashi Torii ◽  
Yuko Uebayashi ◽  
Masao Nasu
Keyword(s):  
Escherichia Coli ◽  
Microfluidic Device ◽  
Potable Water ◽  
Pond Water ◽  
Bacterial Cells ◽  
On Chip

ABSTRACTA microfluidic device-based system for the rapid and semiautomated counting of bacteria in freshwater was fabricated and examined. Bacteria in groundwater and in potable water, as well as starvedEscherichia coliO157:H7 spiked in pond water, were able to be on-chip stained and enumerated within 1 h using this system.

Selective isolation of bacterial cells within a microfluidic device using magnetic probe-based cell fishing

2014 ◽  
Vol 195 ◽  
pp. 581-589 ◽  
Author(s):  
Jérémy Pivetal ◽  
Sylvain Toru ◽  
Marie Frenea-Robin ◽  
Naoufel Haddour ◽  
Sébastien Cecillon ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Magnetic Probe ◽  
Bacterial Cells ◽  
Selective Isolation
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