Sweat Lactic Acid Biosensor using Continuous Flow as Sample Career

Author(s):  
Yoshiki TOJYO ◽  
Haruna SAITO ◽  
Keigo ENOMOTO ◽  
Hiroyuki KUDO
Keyword(s):  
1966 ◽  
Vol 12 (8) ◽  
pp. 497-504 ◽  
Author(s):  
Seymour Winsten ◽  
Jeanne Jackson ◽  
Paula Wolf

Abstract Large- scale, microglass bead, continuous- flow electrophoresis was used to separateand collect protein fractions from a single serum sample. These fractions were assayed for a multiplicity of enzyme activities. Relatively good recovery was obtained with lactic acid dehydrogenase, leucine aminopeptidase, and phosphohexoseisomerase. Artifactually high recoveries were observed with aldotase and isocitric acid dehydrogenase. The increase observed in aldolase activity may be due to a pH effect.


2014 ◽  
Vol 43 (4) ◽  
pp. 535-537 ◽  
Author(s):  
Toshinori Shimanouchi ◽  
Shouhei Ueno ◽  
Kazuki Shidahara ◽  
Yukitaka Kimura

RSC Advances ◽  
2020 ◽  
Vol 10 (52) ◽  
pp. 31123-31138
Author(s):  
Arthur M. Bruno ◽  
Thiago D. R. Simões ◽  
Mariana M. V. M. Souza ◽  
Robinson L. Manfro

The production of lactic acid (LA) from glycerol in alkaline medium was investigated using Cu catalysts supported on CaO, MgO and xCaO/MgO (x = 5, 10, 15 wt%), employing a continuous flow reaction system over a period of 30 h.


Author(s):  
Guangyi Zhang ◽  
Fangming Jin ◽  
Bing Wu ◽  
Jianglin Cao ◽  
Yousif S. Adam ◽  
...  

Abstract Using a self-established laboratory scale continuous-flow reactor, the experimental research on continuous hydrothermal conversion of glycerin into lactic acid was made, to lay a foundation for quantity production of lactic acid from glycerin. The optimal operation condition for producing lactic acid in the continuous-flow reactor, and a ~60% lactic acid yield was achieved. Bad mixing of the reactants as well as a large surface area of the inner wall of the reactor tube decreased significantly the lactic acid yields, especially with a long residence time under a high concentration of alkali. The continuous-flow reactor was modified by substituting the 1/8’’ tube with a 1/4’’ tube and equipping a mixing device, and was tested to perform as well as the one with a 1/8’’ tube. The research results would help design and establish a more high-efficient continuous-flow reactor for hydrothermal conversion of glycerin into lactic acid.


2018 ◽  
Vol 118 ◽  
pp. 160-171 ◽  
Author(s):  
Arthur M. Bruno ◽  
Carlos Alberto Chagas ◽  
Mariana M.V.M. Souza ◽  
Robinson L. Manfro

2016 ◽  
Vol 103 ◽  
pp. 143-147 ◽  
Author(s):  
Leo Gracin ◽  
Anet Režek Jambrak ◽  
Hrvoje Juretić ◽  
Slaven Dobrović ◽  
Irena Barukčić ◽  
...  

2021 ◽  
Vol 24 ◽  
Author(s):  
Vitor Brito ◽  
Renata Santos ◽  
Jeniffer Narcisa-Oliveira ◽  
Ivo Demiate ◽  
Marney Cereda

Abstract The Brazilian fermented cassava starch (polvilho azedo) is prized as a raw material for fast food preparation. Its traditional production by natural fermentation and exposure to sunlight favors contamination and impurities, in addition to depending on the climate. The literature explains the characteristic expansion of polvilho azedo as a reaction of the lactic acid produced during fermentation and sun radiation. With the aim to evaluate a commercial continuous flow process, a modular lamp (UV-C) was evaluated to substitute exposure to sunlight. The selected doses were 1,000, 5,000 and 10,000 Joules per liter (J l-1). Native cassava starch suspension in water at 6% (dry basis) was adjusted to 4% lactic acid (dry basis) and exposed to UV-C radiation: (a) without lactic acid, (b) with lactic acid reaction for 1 hour, (c) with ultraviolet radiation, in addition to the combination of (ac) and (bc). The results showed that only the combination of acidification and ultraviolet radiation (bc) allows an expansion (11 cm3 g-1) higher than the commercial polvilho azedo (8.24 cm3 g-1) adopted as standard. It was concluded that is possible to achieve a substitute for fermented cassava starch by acidification of native cassava starch with lactic acid in a continuous flow system starting with a dose around 5,000 J l-1 of UV-C radiation.


Synthesis ◽  
2017 ◽  
Vol 50 (04) ◽  
pp. 723-741 ◽  
Author(s):  
Christophe Len ◽  
Frederic Delbecq ◽  
Cristobal Cara Corpas ◽  
Encarnacion Ruiz Ramos

This report highlights the recent advances for glycerol valorization to valuable products in liquid phase continuous flow systems using different types of catalysts and processes. The main biobased chemicals obtained from glycerol, such as acrolein, lactic acid, dihydroxyacetone, propanediols, glycerol carbonate, solketal, acetin, alkyl ethers, and oligomers, will be presented.1 Introduction2 Continuous Dehydration2.1 Without Added Catalyst2.2 With Acid Catalyst3 Continuous Oxidation4 Continuous Hydrogenolysis5 Continuous Carbonatation6 Continuous Ketalization7 Continuous Esterification8 Continuous Etherification9 Continuous Oligomerization10 Outlook11 Conclusion


Author(s):  
A. W. Sedar ◽  
G. H. Bresnick

After experimetnal damage to the retina with a variety of procedures Müller cell hypertrophy and migration occurs. According to Kuwabara and others the reactive process in these injuries is evidenced by a marked increase in amount of glycogen in the Müller cells. These cells were considered originally supporting elements with fiber processes extending throughout the retina from inner limiting membrane to external limiting membrane, but are known now to have high lactic acid dehydrogenase activity and the ability to synthesize glycogen. Since the periodic acid-chromic acid-silver methenamine technique was shown to demonstrate glycogen at the electron microscope level, it was selected to react with glycogen in the fine processes of the Müller cell that ramify among the neural elements in various layers of the retina and demarcate these cells cytologically. The Rhesus monkey was chosen as an example of a well vascularized retina and the rabbit as an example of a avascular retina to explore the possibilities of the technique.


2020 ◽  
Vol 22 (19) ◽  
pp. 6437-6443
Author(s):  
Cheng-Kou Liu ◽  
Meng-Yi Chen ◽  
Xin-Xin Lin ◽  
Zheng Fang ◽  
Kai Guo

A catalyst-, oxidant-, acidic solvent- and quaternary ammonium salt-free electrochemical para-selective hydroxylation of N-arylamides at rt in batch and continuous-flow was developed.


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