Three-dimensional reconstruction of blood vessel network by weighted fusion between B-mode and Doppler-mode in ultrasound volumes

2015 ◽  
Author(s):  
Tomoki Yamashita ◽  
Tuan Hung Phan ◽  
Shinya Onogi ◽  
Takashi Mochizuki ◽  
Kohij Masuda
Keyword(s):  
Blood Vessel ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Weighted Fusion ◽  
Dimensional Reconstruction ◽  
Vessel Network ◽  
Blood Vessel Network

scholarly journals Three-Dimensional Reconstruction of Two-Dimensional Cardiovascular Angiography Image Sequences by Local Threshold Segmentation Algorithm

2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Shenming Yu
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Three Dimensional ◽  
Segmentation Algorithm ◽  
Two Dimensional ◽  
Three Dimensional Reconstruction ◽  
Gaussian Smoothing ◽  
Threshold Segmentation ◽  
Local Threshold ◽  
Dimensional Reconstruction

The study focused on the extraction of cardiovascular two-dimensional angiography sequences and the three-dimensional reconstruction based on the local threshold segmentation algorithm. Specifically, the two-dimensional cardiovascular angiography sequence was extracted first, and Gaussian smoothing was adopted for image preprocessing. Then, optimize maximum between-class variance (OSTU) was compared with the traditional two-dimensional OSTU and fast two-dimensional OSTU and applied in the segmentation of cardiovascular angiography images. It was found that the cardiovascular structure itself was continuous, the contrast agent diffused relatively evenly in the blood vessel, and the gray level of the blood vessel was also continuous. The degree of smoothness was consistent in all directions by Gaussian smoothing, avoiding the direction deviation of the smoothened image. The operation time (0.59 s) of the optimize OSTU was significantly shorter than that of traditional OSTU (35.68 s) and fast two-dimensional OSTU (6.34 s) ( P < 0.05 ). The local threshold segmentation algorithm can realize the continuous edge extraction of blood vessels and accurately reflect the stenosis of blood vessels. The results of blood vessel diameter measurement showed that the diameter from the end of blood vessel to the intersection varied linearly from 5.5 mm to 9.0 mm. In short, the optimize OSTU demonstrated good segmentation effects and fast calculation time; it successfully extracted continuous two-dimensional cardiovascular angiography images and can be used in three-dimensional reconstruction of cardiovascular images.

“Open-top” microfluidic device for in vitro three-dimensional capillary beds

Lab on a Chip ◽  
2017 ◽  
Vol 17 (20) ◽  
pp. 3405-3414 ◽  
Author(s):  
Soojung Oh ◽  
Hyunryul Ryu ◽  
Dongha Tahk ◽  
Jihoon Ko ◽  
Yoojin Chung ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Microfluidic Device ◽  
Three Dimensional ◽  
Vessel Network ◽  
Blood Vessel Network

We introduce a novel microfluidic device to co-culture a blood vessel network and cell tissues in an in vivo-like niche.

Methods for three-dimensional reconstruction of cellular components within a thick section

1986 ◽  
Vol 44 ◽  
pp. 18-21
Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder
Keyword(s):  
Tilt Angle ◽  
Tomographic Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Thick Section ◽  
Three Dimensional Reconstruction ◽  
Axis Ratio ◽  
Dimensional Reconstruction ◽  
Cellular Components

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.

Three-Dimensional Reconstruction of Native Androctonus Australis Hemocyanin

1990 ◽  
Vol 48 (1) ◽  
pp. 452-453
Author(s):  
Nicolas Boisset ◽  
Jean-Christophe Taveau ◽  
Jean Lamy ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Solid Body ◽  
Body Surface ◽  
Three Dimensional ◽  
Staining Technique ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
View Reconstruction ◽  
Surface Representations ◽  
Top View

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.

Three-Dimensional Reconstruction of Two Forms of the Chaperonin GRO EL

1990 ◽  
Vol 48 (1) ◽  
pp. 258-259
Author(s):  
J.L. Carrascosa ◽  
G. Abella ◽  
S. Marco ◽  
M. Muyal ◽  
J.M. Carazo
Keyword(s):  
Three Dimensional ◽  
The Other ◽  
Two Dimensional ◽  
Series Method ◽  
Three Dimensional Reconstruction ◽  
Structural Components ◽  
E Coli ◽  
Dimensional Reconstruction ◽  
Morphogenetic Factors ◽  
Tilt Series

Chaperonins are a class of proteins characterized by their role as morphogenetic factors. They trantsiently interact with the structural components of certain biological aggregates (viruses, enzymes etc), promoting their correct folding, assembly and, eventually transport. The groEL factor from E. coli is a conspicuous member of the chaperonins, as it promotes the assembly and morphogenesis of bacterial oligomers and/viral structures.We have studied groEL-like factors from two different bacteria:E. coli and B.subtilis. These factors share common morphological features , showing two different views: one is 6-fold, while the other shows 7 morphological units. There is also a correlation between the presence of a dominant 6-fold view and the fact of both bacteria been grown at low temperature (32°C), while the 7-fold is the main view at higher temperatures (42°C). As the two-dimensional projections of groEL were difficult to interprete, we studied their three-dimensional reconstruction by the random conical tilt series method from negatively stained particles.

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