Gene expression inhibition of N-Myc downregulated gene 1 (NDRG1) monitoring and facilitation via transfectional transfer of NDRG1-siRNA constructs into- in vitro-cultured human glioblastoma cells

2011 ◽  
Author(s):  
Harun M. Said ◽  
Buelent Polat ◽  
Mathias Guckenberger ◽  
Adrian Staab ◽  
Carsten Hagemann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Downregulated Gene ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

scholarly journals A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1249
Author(s):  
Alberto Azzalin ◽  
Francesca Brambilla ◽  
Eloisa Arbustini ◽  
Katia Basello ◽  
Attilio Speciani ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Membrane Trafficking ◽  
Adaptor Protein ◽  
Glucose Starvation ◽  
Glioblastoma Cells ◽  
Glucose Addition ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells ◽  
Inducible Protein

Adaptation of glioblastoma to caloric restriction induces compensatory changes in tumor metabolism that are incompletely known. Here we show that in human glioblastoma cells maintained in exhausted medium, SHC adaptor protein 3 (SHC3) increases due to down-regulation of SHC3 protein degradation. This effect is reversed by glucose addition and is not present in normal astrocytes. Increased SHC3 levels are associated to increased glucose uptake mediated by changes in membrane trafficking of glucose transporters of the solute carrier 2A superfamily (GLUT/SLC2A). We found that the effects on vesicle trafficking are mediated by SHC3 interactions with adaptor protein complex 1 and 2 (AP), BMP-2-inducible protein kinase and a fraction of poly ADP-ribose polymerase 1 (PARP1) associated to vesicles containing GLUT/SLC2As. In glioblastoma cells, PARP1 inhibitor veliparib mimics glucose starvation in enhancing glucose uptake. Furthermore, cytosol extracted from glioblastoma cells inhibits PARP1 enzymatic activity in vitro while immunodepletion of SHC3 from the cytosol significantly relieves this inhibition. The identification of a new pathway controlling glucose uptake in high grade gliomas represents an opportunity for repositioning existing drugs and designing new ones.

An in vitro study on the antitumor effect of sonodynamic therapy using sinoporphyrin sodium on human glioblastoma cells

Ultrasonics ◽  
2021 ◽  
Vol 110 ◽  
pp. 106272
Author(s):  
Yuanyuan Shen ◽  
Yiling Chen ◽  
Yongpeng Huang ◽  
Xiaojun Zeng ◽  
Lanhui Huang ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Glioblastoma Cells ◽  
Sonodynamic Therapy ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

Down-regulation of ribosomal protein S15A inhibits proliferation of human glioblastoma cells in vivo and in vitro via AKT pathway

Tumor Biology ◽  
2015 ◽  
Vol 37 (4) ◽  
pp. 4979-4990 ◽  
Author(s):  
Yiqun Yao ◽  
Yongjian Liu ◽  
Xiupeng Lv ◽  
Bin Dong ◽  
Feng Wang ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Akt Pathway ◽  
Glioblastoma Cells ◽  
Down Regulation ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

scholarly journals Effects of X-irradiation Alone and in Combination with ACNU on Human Glioblastoma Cells in Vitro

1990 ◽  
Vol 30 (5) ◽  
pp. 295-300 ◽  
Author(s):  
Shoji MASHIYAMA ◽  
Ryuichi KATAKURA ◽  
Kou TAKAHASHI ◽  
Masakazu KITAHARA ◽  
Jiro SUZUKI ◽  
...  
Keyword(s):  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
X Irradiation ◽  
Human Glioblastoma Cells

scholarly journals CYTOTOXICITY AND HEMOCOMPATIBILITY OF DOXORUBICIN-LOADED PLGA NANOPARTICLES

2020 ◽  
Vol 19 (1) ◽  
pp. 71-80
Author(s):  
Yu. A. Malinovskaya ◽  
E. I. Kovalenko ◽  
T. S. Kovshova ◽  
N. S. Osipova ◽  
O. O. Maksimenko ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Coagulation ◽  
Hemoglobin Concentration ◽  
Coagulation Cascade ◽  
Coagulation System ◽  
Intracranial Tumour ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

Introduction. The use of polymeric biodegradable nanoparticles (NP) as drug delivery systems is a promising approach to overcome histohematomatic barriers. Thus, poloxamer 188-coated poly (lactide-co-glycolide) (PLGA) NP are able to overcome blood-brain barrier and to deliver therapeutic agents, in particular doxorubicin, into intracranial tumour upon intravenous administration. It is important to evaluate NP interaction with blood components in preclinical studies.The objective of the study was to investigate cytotoxicity and hemocompatibility of doxorubicin-loaded PLGA NP (Dox-PLGA NP), to essess NP uptake by glioblastoma cells.Materials and methods. The influence of NP on coagulation cascade was evaluated by prothrombin time measuring before and after plasma incubation with NP. To assess NP thrombogenicity the platelet activation level was determined by flow cytometry. The NP hemolytic activity (released hemoglobin concentration) was measured spectrophotometrically. NP cytotoxicity was determined by MTS assay. NP uptake by human glioblastoma cells was evaluated by flow cytometry.Results. Dox-PLGA NP did not influence blood coagulation time and thrombocyte activity at concentrations up to 100 mcg/mL: PT values were 12–15 s for all tested samples, and P-selectin expression level did not exceed 15 %. All samples were not hemolytic after 3 h of incubation. Cytotoxicity of doxorubicin released from PLGA NP on glioma U87MG cells was comparable to that of free doxorubicin. As shown by flow cytometry Dox-PLGA NP were efficiently internalized into the cells.Conclusion. The study of hemocompatibility confirmed the safety of Dox-PLGA NP: NP did not influence blood coagulation system and did not induce hemolysis. NP were efficiently internalized into the human glioblastoma cells and produced considerable antitumor effect in vitro.

scholarly journals Disulfiram, a Re-positioned Aldehyde Dehydrogenase Inhibitor, Enhances Radiosensitivity of Human Glioblastoma Cells In Vitro

2019 ◽  
Vol 51 (2) ◽  
pp. 696-705 ◽  
Author(s):  
Hyeon Kang Koh ◽  
Soo Yeon Seo ◽  
Jin Ho Kim ◽  
Hak Jae Kim ◽  
Eui Kyu Chie ◽  
...  
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

scholarly journals Long-term In Vitro Treatment of Human Glioblastoma Cells with Temozolomide Increases Resistance In Vivo through Up-regulation of GLUT Transporter and Aldo-Keto Reductase Enzyme AKR1C Expression

Neoplasia ◽  
2010 ◽  
Vol 12 (9) ◽  
pp. 727-739 ◽  
Author(s):  
Benjamin Le Calvé ◽  
Michal Rynkowski ◽  
Marie Le Mercier ◽  
Céline Bruyère ◽  
Caroline Lonez ◽  
...  
Keyword(s):  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells ◽  
In Vitro Treatment

Investigating the Therapeutic Effects of Alginate Nanogel Co-loaded with Gold Nanoparticles and Cisplatin on U87-MG Human Glioblastoma Cells

2018 ◽  
Vol 18 (6) ◽  
pp. 882-890 ◽  
Author(s):  
Ali Neshastehriz ◽  
Maziar Khateri ◽  
Habib Ghaznavi ◽  
Ali Shakeri-Zadeh
Keyword(s):  
Flow Cytometry ◽  
Gold Nanoparticles ◽  
Survival Rate ◽  
Therapeutic Effects ◽  
X Rays ◽  
Glioblastoma Cells ◽  
Pilot Studies ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

Background and Purpose: It has been well-known both gold nanoparticles (AuNPs) and cisplatin are potential radiosensitizers for radiotherapy of cancer. In this in vitro study, we investigated the chemoradiotherapeutic effects of alginate nanogel co-loaded with AuNPs and cisplatin (ACA) on U87-MG human glioblastoma cells. Methods: Based on the accomplished pilot studies, U87-MG cells were incubated with ACA and cisplatin at 10% inhibitory concentration (IC10) for 4h. Then, the cells were irradiated to different doses of 6MV X-rays (2 and 10 Gy). MTT assay was performed to evaluate the cell survival rate. Apoptosis was determined by flow cytometry using an annexinV–fluorescein isothiocyanate/propidium iodide apoptosis detection kit. Results: The results showed that ACA at the concentration of 4 µg/ml (per cisplatin) and free cisplatin at concentration of 15 μg/ml have the same effects on U87-MG cells (survival rate: 90%). The combination of ACA with radiation resulted in a significant decrease in cell viability (survival rate: 30%). The flow cytometry assay also showed that such a combination therapy induces more apoptosis than necrosis. Conclusion: It may be concluded that co-delivery of AuNPs and cisplatin with a single nanoplatform like ACA nanocomplex enhances the therapeutic ratio of human glioblastoma radiation therapy.

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