Immobilization of bovine serum albumin onto spherical polyelectrolyte brushes in aqueous solution

2011 ◽  
Author(s):  
Siyi Wang ◽  
Yuanqing Zhu ◽  
Kaimin Chen ◽  
Li Li ◽  
Xuhong Guo
Keyword(s):  
Aqueous Solution ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polyelectrolyte Brushes ◽  
Spherical Polyelectrolyte Brushes

scholarly journals Spherical polyelectrolyte brushes as carrier particles for proteins: An investigation of the structure of adsorbed and desorbed bovine serum albumin

2004 ◽  
Vol 18 (2) ◽  
pp. 289-299 ◽  
Author(s):  
G. Jackler ◽  
A. Wittemann ◽  
M. Ballauff ◽  
C. Czeslik
Keyword(s):  
Ionic Strength ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Bovine Serum ◽  
Tertiary Structure ◽  
General View ◽  
Adsorbed State ◽  
Polyelectrolyte Brushes ◽  
Spherical Polyelectrolyte Brushes

The change in the secondary and tertiary structure of bovine serum albumin (BSA) induced by the interaction with spherical polyelectrolyte brushes (SPB) has been investigated using fluorescence and circular dichroism (CD) spectroscopy. The SPB consist of poly(acrylic acid) chains grafted to a poly(styrene) core. The colloidal SPB represent a new substrate for protein immobilization because their protein binding capacity can be controlled by the ionic strength of the solution: SPB bind large amounts of BSA at low ionic strength (pH=6.1), but they are largely protein resistant at moderate salt concentrations of 500 mM. The conformation of BSA which was labeled with the environmentally sensitive dansyl fluorophore was studied before adsorption to the SPB, in the adsorbed state, and after desorption from the SPB. In the adsorbed state the obtained fluorescence spectrum is red-shifted which indicates a hydration of the dansyl fluorophores due to a distortion of the tertiary structure of BSA. Fluorescence and CD spectroscopic analysis of BSA that was desorbed from the SPB shows that the adsorption-induced conformational changes are largely reversible. Convex constraint analysis of the observed CD spectra of BSA yield α-helix fractions of 68% and 57% before adsorption to and after desorption from the SPB, respectively. In a general view, the results of this study demonstrate that spherical polyelectrolyte brushes are suitable for a controlled immobilization and release of proteins without major conformational changes.

Exploration of interactions between decyl-β-d-glucopyranoside and bovine serum albumin in aqueous solution

RSC Advances ◽  
2016 ◽  
Vol 6 (24) ◽  
pp. 19700-19706 ◽  
Author(s):  
Gongke Wang ◽  
Huimin Hou ◽  
Ye Chen ◽  
Changling Yan ◽  
Guangyue Bai ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Surface Tension ◽  
Steady State ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Absorption Spectroscopy ◽  
Bovine Serum ◽  
Aqueous Media ◽  
Steady State Fluorescence

The interactions between decyl-β-d-glucopyranoside (DG) and bovine serum albumin (BSA), in aqueous media, were investigated through the use of surface tension, steady-state fluorescence, and UV-vis absorption spectroscopy measurements.

scholarly journals Thermally induced conformational changes and protein–protein interactions of bovine serum albumin in aqueous solution under different pH and ionic strengths as revealed by SAXS measurements

2017 ◽  
Vol 19 (26) ◽  
pp. 17143-17155 ◽  
Author(s):  
Dmitry Molodenskiy ◽  
Evgeny Shirshin ◽  
Tatiana Tikhonova ◽  
Andrey Gruzinov ◽  
Georgy Peters ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Interaction Potential ◽  
Protein Interactions ◽  
Bovine Serum ◽  
Protein Protein Interactions ◽  
Thermally Induced ◽  
Before And After

Temperature-induced oligomerization of albumin before and after protein melting was studied using SAXS and interpreted in terms of interaction potential.

Insights into the interaction of Bovine Serum Albumin with Surface-Active Ionic Liquids in aqueous solution

2020 ◽  
pp. 114537
Author(s):  
Márcia M.S. Alves ◽  
João M.M. Araújo ◽  
Ivo C. Martins ◽  
Ana B. Pereiro ◽  
Margarida Archer
Keyword(s):  
Aqueous Solution ◽  
Ionic Liquids ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Surface Active
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