Characterization of an novel extracellular biopolymer BC11 by scanning electron microscopy and FT-IR spectroscopy and its use for lead removal

2010 ◽  
Author(s):  
Yanli Mao ◽  
Xiaocun Xiao ◽  
Zhipeng Lu ◽  
Shitian Luo ◽  
Xiaolin Lv ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ir Spectroscopy ◽  
Lead Removal ◽  
Ft Ir ◽  
Scanning Electron ◽  
Ft Ir Spectroscopy

Characterization of banana peel by scanning electron microscopy and FT-IR spectroscopy and its use for cadmium removal

2008 ◽  
Vol 66 (2) ◽  
pp. 260-265 ◽  
Author(s):  
Jamil R. Memon ◽  
Saima Q. Memon ◽  
M.I. Bhanger ◽  
G. Zuhra Memon ◽  
A. El-Turki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ir Spectroscopy ◽  
Banana Peel ◽  
Cadmium Removal ◽  
Ft Ir ◽  
Scanning Electron ◽  
Ft Ir Spectroscopy

scholarly journals Molecular spectroscopy analysis of the substitution of bone tissue by HAp/PLLA composite biomaterial

2004 ◽  
Vol 18 (4) ◽  
pp. 553-565 ◽  
Author(s):  
Nenad Ignjatovic ◽  
Dragan Uskokovic
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Scanning Electron Microscopy ◽  
Ir Spectroscopy ◽  
Molecular Spectroscopy ◽  
Absorption Bands ◽  
Molecular Weights ◽  
Ft Ir ◽  
Scanning Electron ◽  
Ft Ir Spectroscopy

Due to its pronounced osteoinductive properties, calcium hydroxyapatite (HAp) has been widely used in medicine. Bioresorptive poly-L-lactide (PLLA) as a polymer biomaterial has been also used extensively in medicine for its non-toxicity and biocompatibility. To combine the advantages exhibited by each of these materials, a HAp/PLLA composite biomaterial has been synthesized and used for reconstruction and repair of bone defects. Hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial with PLLA of 50,000 and 430,000 g/mole molecular weight was studiedin vivo. The biocomposite with PLLA of both molecular weights was implanted into mice, then removed from their organisms and analyzed by the Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and histopathologic analysis. Characteristic absorption bands, registered and defined by FT-IR spectroscopy, confirm the formation of new functional groups and compounds during the bone repair process using HAp/PLLA biocomposite with PLLA of 50,000 and 430,000 molecular weights. Analysis of the microstructures of the sample surfaces by scanning electron microscopy (SEM) before and after implantation revealed bioresorption of the PLLA polymer phase in the system with PLLA of lower molecular weight and generation of collagen fibers at the sites of implanted bioresorptive PLLA. As the studied synthetic materials behave as the natural bone, i.e., they are phagocytosed and resorpable, they can be considered as biocompatible.

Electrochemical Polymerization of N-(9-Fluorenylmethoxycarbony)-Glycine and Characterization of its Polymers

2011 ◽  
Vol 306-307 ◽  
pp. 297-300
Author(s):  
Yu He ◽  
Wen Juan Guo ◽  
Guang You Zhang ◽  
Mei Shan Pei ◽  
Qi Wei Pan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Boron Trifluoride ◽  
Electrochemical Polymerization ◽  
Supporting Electrolyte ◽  
Oxidation Potential ◽  
Ft Ir ◽  
Scanning Electron ◽  
Ft Ir Spectroscopy

Poly(N-(9-fluorenylmethoxycarbony)-glycine) (PFG) film was first synthesized on platinum wire by direct anodic oxidation of N-(9-fluorenylmethoxycarbony)-glycine (FG) in boron trifluoride diethyl etherate (BFEE). The oxidation potential of FG in BFEE was only 0.4 V vs. Pt. Therefore, BFEE can be an ideal supporting electrolyte for polymerization of FG. Scanning electron microscopy showed the morphology of PFG. The structure of PFG film was studied by FT-IR spectroscopy.

Delamination of Kaolinite by Intercalation of Urea Using Milling

2017 ◽  
Vol 888 ◽  
pp. 136-140 ◽  
Author(s):  
Suzi Salwah Jikan ◽  
Nur Azam Badarulzaman ◽  
Shehu Yahaya ◽  
Ajiya Dahiru Adamu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Powder Diffraction ◽  
Basal Spacing ◽  
X Ray ◽  
Ft Ir ◽  
Scanning Electron ◽  
Ft Ir Spectroscopy

The effect of milling on structure of kaolinite-urea intercalates were studied. Untreated and treated kaolinite samples were examined by Field scanning electron microscopy (FESEM), X-ray powder diffraction (XRPD) and Fourier transform infrared (FT-IR) spectroscopy. The basal spacing of kaolinite measured by X-ray powder diffraction (XRPD) increased from 1.02 to 3.62 nm after intercalation by urea. Significantly, nature of intercalation was reached through formation of hydrogen bonds between urea and both Si-O and AlOH groups of the interlayer surface of kaolinite.

Isolasi Dan Karakterisasi Bentonit Alam Menjadi Nanopartikel Monmorillonit

2018 ◽  
Vol 3 (1) ◽  
pp. 12 ◽  
Author(s):  
Zaimahwati Zaimahwati ◽  
Yuniati Yuniati ◽  
Ramzi Jalal ◽  
Syahman Zhafiri ◽  
Yuli Yetri
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Particle Size ◽  
Surface Morphology ◽  
Average Diameter ◽  
Particle Size Analyzer ◽  
Ft Ir ◽  
Scanning Electron ◽  
Sedimentation Processes

<p>Pada penelitian ini telah dilakukan isolasi dan karakterisasi bentonit alam menjadi nanopartikel montmorillonit. Bentonit alam yang digunakan diambil dari desa Blangdalam, Kecamatan Nisam Kabupaten Aceh Utara.  Proses isolasi meliputi proses pelarutan dengan aquades, ultrasonic dan proses sedimentasi. Untuk mengetahui karakterisasi montmorillonit dilakukan uji FT-IR, X-RD dan uji morfologi permukaan dengan Scanning Electron Microscopy (SEM). Partikel size analyzer untuk menganalisis dan menentukan ukuran nanopartikel dari isolasi bentonit alam. Dari hasil penelitian didapat ukuran nanopartikel montmorillonit hasil isolasi dari bentonit alam diperoleh berdiameter rata-rata 82,15 nm.</p><p><em>In this research we have isolated and characterized natural bentonite into montmorillonite nanoparticles. Natural bentonite used was taken from Blangdalam village, Nisam sub-district, North Aceh district. The isolation process includes dissolving process with aquades, ultrasonic and sedimentation processes.  The characterization of montmorillonite, FT-IR, X-RD and surface morphology test by Scanning Electron Microscopy (SEM). Particle size analyzer to analyze and determine the size of nanoparticles from natural bentonite insulation. From the research results obtained the size of montmorillonite nanoparticles isolated from natural bentonite obtained an average diameter of 82.15 nm.</em></p>

The Characterization of Bovine Bone-Derived Hydroxyapatite Isolated Using Novel Non-Hazardous Method

2020 ◽  
Vol 45 ◽  
pp. 49-56
Author(s):  
Aniek Setiya Budiatin ◽  
Samirah ◽  
Maria Apriliani Gani ◽  
Wenny Putri Nilamsari ◽  
Chrismawan Ardianto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Extraction Method ◽  
Bovine Bone ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ft Ir ◽  
Novel Method ◽  
Scanning Electron

Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800°C, 900°C and 1,000°C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 ± 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.

scholarly journals Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

2016 ◽  
Vol 82 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
Agustin Sri MULYATNI ◽  
Deden Dewantara ERIS ◽  
Haryo Tejo PRAKOSO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Palm Oil ◽  
Ft Ir ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil  PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan  karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh  tiga isolat potensial, yaitu : satu isolat  Pseudo-monas aeruginosa dan dua isolat  Bacillus subtilis.  Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa  berbeda dengan  Bacillus subtilisyang ditandai   perbedaan   pada  spectra   gugus  C-C  dan   C-H alifatik.  Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi  enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

scholarly journals Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

2016 ◽  
Vol 82 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
Agustin Sri MULYATNI ◽  
Deden Dewantara ERIS ◽  
Haryo Tejo PRAKOSO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Palm Oil ◽  
Ft Ir ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil  PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan  karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh  tiga isolat potensial, yaitu : satu isolat  Pseudo-monas aeruginosa dan dua isolat  Bacillus subtilis.  Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa  berbeda dengan  Bacillus subtilisyang ditandai   perbedaan   pada  spectra   gugus  C-C  dan   C-H alifatik.  Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi  enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

Sign in / Sign up

Export Citation Format

Share Document