Muscle Reconstruction and Regeneration Using Biodegradable Scaffolds

2010 ◽  
Author(s):  
Cristiano Spadaccio ◽  
Alberto Rainer ◽  
Stefano De Porcellinis ◽  
Federico De Marco ◽  
Massimo Chello ◽  
...  
Keyword(s):  
Biodegradable Scaffolds

In Vitro Degradation of β-TCP/PLLA Scaffolds with Different Proportions of β-TCP

2007 ◽  
Vol 336-338 ◽  
pp. 1545-1548
Author(s):  
Lin Luo ◽  
Guang Fu Yin ◽  
Yun Zhang ◽  
Ya Dong Yao ◽  
Wei Zhong Yang ◽  
...  
Keyword(s):  
Degradation Rate ◽  
Porous Scaffolds ◽  
Carbonate Apatite ◽  
Tissue Formation ◽  
Cellular Attachment ◽  
Biodegradable Scaffolds ◽  
Scaffold Structure ◽  
Mechanical Strengths

Porous biodegradable scaffolds are widely used in bone tissue engineering to provide temporary templates for cellular attachment and matrix synthesis. Ideally, the degradation rate in vivo may be similar or slightly less than that of tissue formation, allowing for the maintenance of the scaffold structure and the mechanical support during early stages of tissue formation. Eventually, the 3-D spaces occupied by the porous scaffolds will be replaced by newly formed tissue. In this work, β-tricalcium phosphate/Poly-L lactide (β-TCP/PLLA) scaffolds with different proportions of β-TCP to PLLA were investigated. The effects of β-TCP proportions on degradation rate and mechanical strengths of the scaffolds were evaluated in simulated body fluid (SBF) at 37°C up to 42 days. Results show that: different proportions of β-TCP to PLLA have significant influence on degradation behaviors of the scaffolds, and mechanical strengths of the scaffolds with weight proportion of β-TCP to PLLA being 2 to 1 are much higher than those of the others during the degradation period. And in this period, the scaffolds biodegrade slowly, and Hydroxyl Carbonate Apatite (HCA) forms in the surface of the material.

Impact of metalloproteinase motifs on the mechanical properties of pegda hydrogels as potential biodegradable scaffolds for wound healing

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S141
Author(s):  
G.A. Bayona ◽  
V.A. Solarte-David ◽  
A.V. Pinzon-Mora ◽  
M.L. Arango-Rodriguez ◽  
S.M. Becerra-Bayona
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Biodegradable Scaffolds

Techniques and materials used to optimize attached gingiva size: literature review

2021 ◽  
Vol 1 (12) ◽  
pp. 42-47
Author(s):  
Z. S. Khabadze ◽  
A. B. Adzhieva ◽  
A. A. Nedashkovsky ◽  
Yu. A. Generalova ◽  
M. G. Sherozia ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Closure ◽  
Soft Tissue Defects ◽  
Autologous Tissue ◽  
Biodegradable Scaffolds ◽  
Attached Gingiva ◽  
Materials Used ◽  
Oral Soft Tissue ◽  
Tissue Defects ◽  
Interesting Alternative

The aim of this review was to examine the techniques for performing keratinized gingival augmentation and grafts, as well as the materials used, which are often required to ensure proper wound closure. Tissue engineering of the oral mucosa represents an interesting alternative to obtain sufficient autologous tissue to repair oral soft tissue defects using biodegradable scaffolds and can improve vascularization and epithelialization, which are critical for successful outcomes.

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

2004 ◽  
Vol 83 (7) ◽  
pp. 523-528 ◽  
Author(s):  
M.T. Duailibi ◽  
S.E. Duailibi ◽  
C.S. Young ◽  
J.D. Bartlett ◽  
J.P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Engineering Applications ◽  
Adult Rat ◽  
Dental Tissues ◽  
Biodegradable Scaffolds ◽  
Bioengineered Tooth ◽  
Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

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