Numerical simulations to analyze the effect of various heater configurations on heat transfer in polymerase chain reaction devices

2017 ◽  
Author(s):  
Rabia Jamshaid ◽  
Imran Aziz
Keyword(s):  
Heat Transfer ◽  
Polymerase Chain Reaction ◽  
Numerical Simulations ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals An Innovative Rapid Thermal Cycling Device for Polymerase Chain Reaction

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Shadi Mahjoob ◽  
Kambiz Vafai
Keyword(s):  
Heat Transfer ◽  
Polymerase Chain Reaction ◽  
Temperature Distribution ◽  
Thermal Cycling ◽  
Thermal Management ◽  
Uniform Temperature ◽  
Chain Reaction ◽  
Temperature Ramp ◽  
Polymerase Chain ◽  
Thermal Cycler

Polymerase chain reaction (PCR) is the most commonly used molecular biology technique to amplify nucleic acid (DNA and RNA) in vitro. This technique is highly temperature sensitive and thermal management has an important role in PCR operation in reaching the required temperature set points at each step of the process (denaturing, annealing and elongation). In this work, an innovative microfluidic PCR thermal cycling device is designed to increase the heating∕cooling thermal cycling speed while maintaining a uniform temperature distribution throughout the substrate containing the aqueous nucleic acid sample. The device design is incorporating the jet impingement and micro-channel thermal management technologies utilizing a properly arranged configuration filled with a porous medium. Porous Inserts are attractive choices in heat transfer augmentation. They provide a very large surface area for a given volume which is a key parameter in heat transfer processes. Various effective parameters that are relevant in optimizing this flexible thermal cycler are investigated such as thermal cycler configuration, thickness of inlet and exit fluid channels, fluid flow rate and velocity, the porous matrix material and properties, and utilization of thermal grease. An optimized case is established based on the effects of the cited parameters on the temperature ramp, temperature distribution and the required power for circulating the fluid in the thermal cycler. The results indicate that the heating∕cooling temperature ramp (temperature change per heating∕cooling cycling time) of the proposed device is considerably higher (150.82◻C∕s) than those in literature. In addition, the proposed PCR offers a very uniform temperature in the substrate while utilizing a low power.

Effect of convection modes on the polymerase chain reaction in a square cavity

2012 ◽  
Vol 9 (2) ◽  
pp. 86-89
Author(s):  
K.V. Moiseyev
Keyword(s):  
Heat Transfer ◽  
Polymerase Chain Reaction ◽  
Concentration Field ◽  
Boussinesq Approximation ◽  
Convection Flow ◽  
Square Cavity ◽  
Chain Reaction ◽  
Physicochemical Interactions ◽  
Polymerase Chain ◽  
Substance Transfer

The effect of various regimes of free-convective heat transfer of a Newtonian incompressible fluid on the polymerase chain reaction in a square cavity is numerically studied. As a mathematical model of the process of free convection, the Oberbeck-Boussinesq approximation is considered. For the polymerase chain reaction, the equations of substance transfer are used, taking into account the physicochemical interactions of the components of the mixture. It was believed that the mixture consists of three components. The results of calculations allow us to estimate the effect of convection flow regimes on the overall duration of the reaction and the concentration field of the components, and also to determine the optimum thermal parameters for PCR.

Computer Simulation Analysis of Microchannel for a Continuous-Flow PCR Chip

2013 ◽  
Vol 364 ◽  
pp. 238-243
Author(s):  
Chao Wang Young ◽  
Cheng Chang Lien ◽  
Chyung Ay ◽  
Pao Chia Pan
Keyword(s):  
Heat Transfer ◽  
Polymerase Chain Reaction ◽  
Cross Sections ◽  
Continuous Flow ◽  
Simulation Analysis ◽  
Main Idea ◽  
Chain Reaction ◽  
Fast Heating ◽  
Heating And Cooling ◽  
Polymerase Chain

The purpose of this study is to analyze the model of a continuous-flow Polymerase Chain Reaction (PCR) chip with CoventorWare software. It offered the ability of fast heating and cooling rate when the DNA duplication process happened. The main idea was to compare the effects of different cross sections of channel and find out the better one for heat transfer.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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