Spectral method simulations of light scattering by biological cells

2001 ◽  
Vol 37 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Y. Shao ◽  
A.T. Maximov ◽  
I.G. Ourdev ◽  
U. Rozmus ◽  
C.E. Capjack
Keyword(s):  
Light Scattering ◽  
Spectral Method ◽  
Biological Cells

Numerical Simulation and Experimental Study on Light Scattering by Biological Cells with Discrete Dipole Approximation

2013 ◽  
Vol 760-762 ◽  
pp. 105-109 ◽  
Author(s):  
Jian Bin Liu ◽  
Hai Li ◽  
Ying Xin Zeng ◽  
Jia Wen Weng ◽  
Chu Ping Yang
Keyword(s):  
Cell Culture ◽  
Light Scattering ◽  
Culture Medium ◽  
Dipole Approximation ◽  
Spherical Particles ◽  
Biological Cells ◽  
Cell Culture Medium ◽  
Animal Cells ◽  
Experimental Apparatus ◽  
Liquid Suspensions

An experimental apparatus for the analysis of biological cells light scattering in liquid suspensions has been presented. Characterization is based on the scattering of a monochromatic laser beam by particles [which can be inorganic, organic, or biological (such as animal cells and bacteria)] and on the strong relation between the light-scattering pattern and the morphology and refractive index of the particles. In order to study light scattering in biological cells close to the actual situation, we focus on non-spherical particles in the cell-culture medium. Finally, we demonstrate the light scattering results of bovine kidney cells suspended in the cell-culture medium, and compares then with the simulated results.

scholarly journals The FDTD approach applied to light scattering from single biological cells

2004 ◽  
Author(s):  
Stoyan Tanev ◽  
Wenbo Sun ◽  
Richard Zhang ◽  
Andrew Ridsdale
Keyword(s):  
Light Scattering ◽  
Biological Cells

Light Scattering Images of Amplitude, Phase and Polarization from Typical Blood Cells

2011 ◽  
Vol 181-182 ◽  
pp. 430-433
Author(s):  
Ya Wei Wang ◽  
Yuan Yuan Xu ◽  
Qu Wei Yue ◽  
Yu Jiao Chen ◽  
Min Bu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Light Scattering ◽  
Blood Cells ◽  
Cell Recognition ◽  
Biological Cells ◽  
Phase Microscopy ◽  
Digital Phase ◽  
Simulation Techniques

With the development of DPM (digital phase microscopy) and FCM (flow cytometry), techniques of cell recognition are getting more and more applications in many fields. Researches on the information from cells are very important to these techniques. In this paper, focusing on the technique of analyzing light scattering information and its relation with the morphological structures of the leucocytes and erythrocytes have been done. Based on the VirtualLab simulation techniques, the light scattering images of amplitude, phase and polarization of these typical cells have been obtained. It is found that the amplitude, phase and polarization of these cells are closely related to their morphological structures by analyzing the images, which is very useful to identify biological cells.

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