Polarized light scattering of nucleosomes and polynucleosomes-in situ and in vitro studies

1991 ◽  
Vol 38 (7) ◽  
pp. 670-678 ◽  
Author(s):  
A. Diaspro ◽  
M. Bertolotto ◽  
L. Vergani ◽  
C. Nicolini
Keyword(s):  
Light Scattering ◽  
Polarized Light ◽  
In Vitro Studies

scholarly journals Effect of Cavity Disinfectants on Adhesion to Primary Teeth—A Systematic Review

2021 ◽  
Vol 22 (9) ◽  
pp. 4398
Author(s):  
Ana Coelho ◽  
Inês Amaro ◽  
Ana Apolónio ◽  
Anabela Paula ◽  
José Saraiva ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Clinical Success ◽  
Dental Materials ◽  
In Vitro Study ◽  
In Vitro Studies ◽  
Cochrane Library ◽  
Promising Alternative

Some authors have been proposing the use of cavity disinfectants in order to reduce, or even eliminate, the effect of the microorganisms present in a dental cavity before a restoration is placed. The aim of this study was to evaluate the effect of different cavity disinfectants on bond strength and clinical success of composite and glass ionomer restorations on primary teeth. The research was conducted using Cochrane Library, PubMed/MEDLINE, SCOPUS, and Web of Science for articles published up to February 2021. The search was performed according to the PICO strategy. The evaluation of the methodological quality of each in vitro study was assessed using the CONSORT checklist for reporting in vitro studies on dental materials. Sixteen in vitro studies and one in situ study fulfilled the inclusion criteria and were analyzed. Chlorhexidine was the most studied cavity disinfectant, and its use does not compromise dentin bonding. Sodium hypochlorite is a promising alternative, but more research on its use is required to clearly state that it can safely be used as a cavity disinfectant for primary teeth. Although other disinfectants were studied, there is a low-level evidence attesting their effects on adhesion, therefore their use should be avoided.

scholarly journals MICROSPECTROPHOTOMETRY OF NEWLY SYNTHESIZED STARCH IN SITU

1970 ◽  
Vol 18 (6) ◽  
pp. 439-449
Author(s):  
GEORGE E. WHEELER
Keyword(s):  
Absorption Maximum ◽  
In Vitro Studies ◽  
Phosphate Solution ◽  
Starch Digestion ◽  
Spectral Curves ◽  
Similarities And Differences

Many of the cells in stem sections of several Commelinaceae species synthesized much new starch when incubated in buffered 1% glucose 1-phosphate solution. The starch appeared in the cytoplasm rather than in the plastids. Although the starch I2-KI color was uniform within any one cell, there was considerable variation from cell to cell, even in the same section. The colors with I2-KI ranged from blue, through purples to magenta and mahogany. Tests with α-amylase and with β-amylase showed the starch to be amylose. Microspectrophotometrically determined extinction curves, based on the new starch in situ, varied with the visualized color. As expected, starch which stained blue with I2-KI had an absorption maximum in the orange-red wavelengths above 600 mµ; increasingly red I2-KI colors were characterized by shifts of the absorption maximum further into the shorter wavelengths. The course of new starch digestion by α-amylase and by β-amylase was followed visually and with the microspectrophotometer. Similarities and differences between these spectral curves and those published for in vitro studies are pointed out. The difficulties met with in using the microspectrophotometric method are discussed.

Hyperspectral polarized light scattering to study tumor cells in in-vitro samples

2012 ◽  
Author(s):  
Nicolas Riviere ◽  
Romain Ceolato ◽  
Corinne Lorenzo ◽  
Raphaël Jorand ◽  
Bernard Ducommun
Keyword(s):  
Light Scattering ◽  
Tumor Cells ◽  
Polarized Light

scholarly journals Mature Dendritic Cells Infiltrate the T Cell-Rich Region of Oral Mucosa in Chronic Periodontitis: In Situ, In Vivo, and In Vitro Studies

2001 ◽  
Vol 167 (8) ◽  
pp. 4693-4700 ◽  
Author(s):  
Ravi Jotwani ◽  
Anna Karolina Palucka ◽  
Montasr Al-Quotub ◽  
Mahyar Nouri-Shirazi ◽  
Jay Kim ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Oral Mucosa ◽  
Chronic Periodontitis ◽  
In Vitro Studies ◽  
Rich Region

scholarly journals In-situ Detection Method for Microplastics in Water by Polarized Light Scattering

2021 ◽  
Vol 8 ◽  
Author(s):  
Tong Liu ◽  
Shijun Yu ◽  
Xiaoshan Zhu ◽  
Ran Liao ◽  
Zepeng Zhuo ◽  
...  
Keyword(s):  
Light Scattering ◽  
Scattered Light ◽  
Polarized Light ◽  
Chemical Properties ◽  
Future Application ◽  
Physical And Chemical Properties ◽  
The Individual ◽  
Physical And Chemical ◽  
And Chemical Properties

Microplastics (MPs) have become the widespread contaminants, which raises concerns on their ecological hazards. In-situ detection of MP in water bodies is essential for clear assessment of the ecological risks of MPs. The present study proposes a method based on polarized light scattering which measures the polarization parameters of the scattered light at 120° to detect MP in water. This method takes the advantage of in-situ measurement of the individual particles and the experimental setup in principle is used. By use of the measured polarization parameters equipped by machine learning, the standard polystyrene (PS) spheres, natural water sample, and lab-cultured microalgae are explicitly discriminated, and MP with different physical and chemical properties can be differentiated. It can also characterize the weathering of different MP and identify the specific type from multiple types of MP. This study explores the capability of the proposed method to detect the physical and chemical properties, weathering state and concentration of MP in water which promises the future application in water quality sensing and monitoring.

scholarly journals 5-Fluorouracil Release from Chitosan-Based Matrix.Experimental and Theoretical Aspects

2020 ◽  
Vol 57 (3) ◽  
pp. 180-188
Author(s):  
Roxana Iancu ◽  
Stefan Andrei Irimiciuc ◽  
Maricel Agop ◽  
Mihail Frasila ◽  
Maria-Alexandra Paun ◽  
...  
Keyword(s):  
Drug Release ◽  
Polarized Light ◽  
Crosslinking Density ◽  
Fickian Diffusion ◽  
Release Mechanism ◽  
Morphological Aspects ◽  
Drug Release Mechanism ◽  
The Matrix

A series of four drug release formulations based on 5-fluorouracil encapsulated into a chitosan-based matrix were prepared by in situ hydrogelation with 3,7-dimethyl-2,6-octadienal. The formulations were investigated from structural and morphological aspects by FTIR spectroscopy, polarized light microscopy and scanning electron microscopy. It was established that 5-fluorouracil was anchored into the matrix as crystals, whose dimension varied as a function of the crosslinking density. The in vitro drug release simulated into a media mimicking the physiological environment revealed a progressive release of the 5-fluorouracil, in close interdependence with the crosslinking density. In the context of Pharmacokinetics behavioral analysis, a new mathematical procedure for describing drug release dynamics in polymer-drug complex system is proposed. Assuming that the dynamics of polymer-drug system�s structural units take place on continuous and nondifferentiable curves (multifractal curves), we show that in a one-dimensional hydrodynamic formalism of multifractal variables the drug release mechanism (Fickian diffusion, non-Fickian diffusion, etc) are given through synchronous dynamics at a differentiable and non-differentiable scale resolutions. Finally, the model is confirmed by the empirical data.

scholarly journals Optimization of the In Situ Laser Fenestration of Stent Graft: In Vitro Studies

2018 ◽  
Vol 68 (3) ◽  
pp. e91
Author(s):  
Jing Lin ◽  
Niraj Parikh ◽  
Lu Wang ◽  
Mark Nutley ◽  
Jean Panneton ◽  
...  
Keyword(s):  
Stent Graft ◽  
In Vitro Studies
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