A method of analyzing nonstationary ionic channel current fluctuations in the presence of an additive measurement noise

1993 ◽  
Vol 40 (3) ◽  
pp. 222-229 ◽  
Author(s):  
H. Mino
Keyword(s):  
Ionic Channel ◽  
Measurement Noise ◽  
Current Fluctuations ◽  
Channel Current

Time-dependent expression of ryanodine receptors in sea urchin eggs, zygotes and early embryos

Zygote ◽  
2021 ◽  
pp. 1-4
Author(s):  
G. Percivale ◽  
C. Angelini ◽  
C. Falugi ◽  
C. Picco ◽  
G. Prestipino
Keyword(s):  
Single Channel ◽  
Ryanodine Receptors ◽  
Paracentrotus Lividus ◽  
Current Fluctuations ◽  
Calcium Dependent ◽  
Channel Current ◽  
Single Channel Current ◽  
Electrophysiological Recordings ◽  
Early Embryos ◽  
Zygote Stage

Summary In this work, the presence of calcium-dependent calcium channels and their receptors (RyR) has been investigated in Paracentrotus lividus eggs and early embryos, from unfertilized egg to four-blastomere stages. Electrophysiological recordings of RyR single-channel current fluctuations showed that RyRs are functional during the first developmental events with a maximum at zygote stage, c. 40 min after fertilization, corresponding to the first cleavage. The nature of vertebrate-like RyRs active at this stage was established by specific activation/blockade experiments.

Ionic Channel Current Burst Analysis by a Machine Learning Based Approach

2011 ◽  
Vol 10 (3) ◽  
pp. 152-159 ◽  
Author(s):  
G. Rauch ◽  
S. Bertolini ◽  
R. Sacile ◽  
M. Giacomini ◽  
C. Ruggiero
Keyword(s):  
Machine Learning ◽  
Ionic Channel ◽  
Channel Current ◽  
Burst Analysis

Recording of a large-conductance chloride channel in normal rat lactotrophs

1995 ◽  
Vol 269 (5) ◽  
pp. E969-E976 ◽  
Author(s):  
M. Fahmi ◽  
L. Garcia ◽  
A. Taupignon ◽  
B. Dufy ◽  
P. Sartor
Keyword(s):  
Reversal Potential ◽  
High Gain ◽  
Ionic Channel ◽  
Current Fluctuations ◽  
Pharmacological Properties ◽  
Patch Clamp Technique ◽  
Nernst Equation ◽  
Whole Cell ◽  
Cell Configuration ◽  
Normal Rat

Membrane current fluctuations resembling channel openings and closings were observed in the whole cell configuration of the patch-clamp technique in normal rat lactotrophs in primary culture. Using high-gain head stage in whole cell configuration, we characterized the nature and pharmacological properties of the ionic channel underlying these fluctuations. This channel, found in small numbers (< 10 per cell), was specific for Cl- because its reversal potential varied with Cl- gradients, according to the Nernst equation, and its unitary amplitude was linearly related to membrane potential from -100 to 0 mV. Slope conductance was close to 100 pS. Analyzing open times, we demonstrated its Ca2+ and potential dependence. Four sublevels were observed. We suggest that this channel, belonging to the background Cl- channel group, takes part in the regulation of intracellular Cl- concentration of normal rat lactotrophs.

Ion channels for communication between and within cells

1992 ◽  
Vol 12 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Erwin Neher
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Single Channel ◽  
Current Fluctuations ◽  
Channel Current ◽  
Single Channel Current

The development of patch-clamp procedures for measuring single-channel current fluctuations are described. The application of these techniques for studying secretion is discussed.

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