Crystallization and preliminary X-ray crystallographic analysis of the putative [6Fe–6S] prismane protein from Desulfovibrio vulgaris (Hildenborough)

1996 ◽  
Vol 52 (6) ◽  
pp. 1211-1213
Author(s):  
A. Arendsen ◽  
Y. Bulsink ◽  
W. Hagen ◽  
J. Hadden ◽  
G. Card ◽  
...  
Keyword(s):  
Crystallographic Analysis ◽  
Desulfovibrio Vulgaris ◽  
X Ray ◽  
Desulfovibrio Vulgaris Hildenborough

scholarly journals Probing the Structure of [NiFeSe] Hydrogenase with QM/MM Computations

2020 ◽  
Vol 10 (3) ◽  
pp. 781 ◽  
Author(s):  
Samah Moubarak ◽  
N. Elghobashi-Meinhardt ◽  
Daria Tombolelli ◽  
Maria Andrea Mroginski
Keyword(s):  
Iron Oxidation ◽  
Structural Models ◽  
Oxidation States ◽  
Quantum Mechanical ◽  
Desulfovibrio Vulgaris ◽  
X Ray ◽  
X Ray Crystallography ◽  
Desulfovibrio Vulgaris Hildenborough ◽  
Optimized Geometries ◽  
Good Agreement

The geometry and vibrational behavior of selenocysteine [NiFeSe] hydrogenase isolated from Desulfovibrio vulgaris Hildenborough have been investigated using a hybrid quantum mechanical (QM)/ molecular mechanical (MM) approach. Structural models have been built based on the three conformers identified in the recent crystal structure resolved at 1.3 Å from X-ray crystallography. In the models, a diamagnetic Ni2+ atom was modeled in combination with both Fe2+ and Fe3+ to investigate the effect of iron oxidation on geometry and vibrational frequency of the nonproteic ligands, CO and CN-, coordinated to the Fe atom. Overall, the QM/MM optimized geometries are in good agreement with the experimentally resolved geometries. Analysis of computed vibrational frequencies, in comparison with experimental Fourier-transform infrared (FTIR) frequencies, suggests that a mixture of conformers as well as Fe2+ and Fe3+ oxidation states may be responsible for the acquired vibrational spectra.

scholarly journals X-ray crystal structure of the Desulfovibrio vulgaris (Hildenborough) apoflavodoxin-riboflavin complex

1998 ◽  
Vol 258 (2) ◽  
pp. 362-371 ◽  
Author(s):  
Martin A. Walsh ◽  
Andrew McCarthy ◽  
Paul A. O'Farrell ◽  
Patrick McArdle ◽  
P. Desmond Cunningham ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Desulfovibrio Vulgaris ◽  
X Ray ◽  
Desulfovibrio Vulgaris Hildenborough

Quaternary structure of Limulus polyphemus hemocyanin

1978 ◽  
Vol 36 (2) ◽  
pp. 176-177
Author(s):  
T. Wichertjes ◽  
E.J. Kwak ◽  
E.F.J. Van Bruggen
Keyword(s):  
Electron Microscopy ◽  
Functional Properties ◽  
Horseshoe Crab ◽  
Temperature Jump ◽  
Quaternary Structure ◽  
Crystallographic Analysis ◽  
Limulus Polyphemus ◽  
Oxygen Binding ◽  
X Ray ◽  
Intact Molecule

Hemocyanin of the horseshoe crab (Limulus polyphemus) has been studied in nany ways. Recently the structure, dissociation and reassembly was studied using electron microscopy of negatively stained specimens as the method of investigation. Crystallization of the protein proved to be possible and X-ray crystallographic analysis was started. Also fluorescence properties of the hemocyanin after dialysis against Tris-glycine buffer + 0.01 M EDTA pH 8.9 (so called “stripped” hemocyanin) and its fractions II and V were studied, as well as functional properties of the fractions by NMR. Finally the temperature-jump method was used for assaying the oxygen binding of the dissociating molecule and of preparations of isolated subunits. Nevertheless very little is known about the structure of the intact molecule. Schutter et al. suggested that the molecule possibly consists of two halves, combined in a staggered way, the halves themselves consisting of four subunits arranged in a square.

Purification, crystallization and X-ray crystallographic analysis of ATPase domain in transcription regulator DmpR

2019 ◽  
Vol 7 (2) ◽  
pp. 52-55
Author(s):  
Su-Jin Lee ◽  
In-Young Baek ◽  
Yan An ◽  
Woo-Chan Ahn ◽  
Kwang-Hyun Park ◽  
...  
Keyword(s):  
Crystallographic Analysis ◽  
Atpase Domain ◽  
X Ray ◽  
Transcription Regulator

Purification, crystallization and X-ray crystallographic analysis of methylglyoxal reductase Gre2 from Candida albicans

2019 ◽  
Vol 7 (2) ◽  
pp. 42-46 ◽  
Author(s):  
Giang Thu Nguyen ◽  
Shinae Kim ◽  
Hang-Suk Chun ◽  
Woo-Keun Kim ◽  
Jeong Ho Chang
Keyword(s):  
Candida Albicans ◽  
Crystallographic Analysis ◽  
X Ray
Sign in / Sign up

Export Citation Format

Share Document