scholarly journals Developmental and hormonal regulation of sunflower helianthinin genes: proximal promoter sequences confer regionalized seed expression.

1994 ◽  
Vol 6 (4) ◽  
pp. 473-486 ◽  
Author(s):  
A N Nunberg ◽  
Z Li ◽  
M A Bogue ◽  
J Vivekananda ◽  
A S Reddy ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Proximal Promoter ◽  
Promoter Sequences ◽  
Seed Expression

Developmental and Hormonal Regulation of Sunflower Helianthinin Genes: Proximal Promoter Sequences Confer Regionalized Seed Expression

1994 ◽  
Vol 6 (4) ◽  
pp. 473
Author(s):  
Andrew N. Nunberg ◽  
Zhuwen Li ◽  
Molly A. Bogue ◽  
Jeevalatha Vivekananda ◽  
Avutu S. Reddy ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Proximal Promoter ◽  
Promoter Sequences ◽  
Seed Expression

Proximal Promoter Sequences of Sunflower Helianthinin Genes Confer Regionalized Seed-Specific Expression

1995 ◽  
Vol 145 (5-6) ◽  
pp. 600-605 ◽  
Author(s):  
Andrew N. Nunberg ◽  
Zhuwen Li ◽  
Hwa-Jee Chung ◽  
Avutu S. Reddy ◽  
Terry L. Thomas
Keyword(s):  
Proximal Promoter ◽  
Specific Expression ◽  
Promoter Sequences ◽  
Seed Specific Expression

scholarly journals The Role of Transcription Factor PU.I in the Activity of the Intronic Enhancer of the Eosinophil-Derived Neurotoxin (RNS2) Gene

Blood ◽  
1998 ◽  
Vol 91 (6) ◽  
pp. 2126-2132 ◽  
Author(s):  
Thamar B. van Dijk ◽  
Eric Caldenhoven ◽  
Jan A.M. Raaijmakers ◽  
Jan-Willem J. Lammers ◽  
Leo Koenderman ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Point Mutations ◽  
Proximal Promoter ◽  
Multiple Forms ◽  
Enhancer Activity ◽  
Promoter Sequences ◽  
Intronic Enhancer ◽  
First Intron ◽  
Shift Analysis

Eosinophil-derived neurotoxin (EDN) found in the granules of human eosinophils is a cationic ribonuclease toxin. Expression of the EDN gene (RNS2) in eosinophils is dependent on proximal promoter sequences in combination with an enhancer located in the first intron. We further define here the active region of the intron using transfections in differentiated eosinophilic HL60 cells. We show that a region containing a tandem PU.I binding site is important for intronic enhancer activity. This region binds multiple forms of transcription factor PU.I as judged by gel-shift analysis and DNA affinity precipitation. Importantly, introducing point mutations in the PU.I site drastically reduces the intronic enhancer activity, showing the importance of PU.I for expression of EDN in cells of the eosinophilic lineage.

Functional interactions of an upstream enhancer of the mouse glycoprotein hormone α-subunit gene with proximal promoter sequences

1998 ◽  
Vol 142 (1-2) ◽  
pp. 141-152 ◽  
Author(s):  
William M Wood ◽  
Janet M Dowding ◽  
Virginia D Sarapura ◽  
Michael T McDermott ◽  
David F Gordon ◽  
...  
Keyword(s):  
Proximal Promoter ◽  
Subunit Gene ◽  
Glycoprotein Hormone ◽  
Α Subunit ◽  
Promoter Sequences ◽  
Functional Interactions

scholarly journals Developmental regulation of lck gene expression in T lymphocytes.

1991 ◽  
Vol 173 (2) ◽  
pp. 383-393 ◽  
Author(s):  
R S Wildin ◽  
A M Garvin ◽  
S Pawar ◽  
D B Lewis ◽  
K M Abraham ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Short Interval ◽  
T Antigen ◽  
Lymphoid Cells ◽  
Proximal Promoter ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Promoter Sequences ◽  
Antigen Gene ◽  
Distal Promoter

In the mouse and human, mRNA transcripts encoding the lymphocyte-specific protein tyrosine kinase p56lck are derived from two separate promoters resulting in heterogeneity in the 5' untranslated region sequence. The proximal promoter lies just 5' to the coding region for the gene and is active only in thymocytes. In contrast, the distal promoter lies 34 kilobases (kb) 5' in the human, and is active both in thymocytes and mature peripheral T cells. As previously reported, transgenic mice bearing functional proximal promoter sequence juxtaposed with the SV40 large T antigen gene invariably develop lymphoid tumors confined to the thymus. In the current work, transgenic mice bearing a 2.6-kb fragment of the human distal promoter fused to the SV40 large T antigen gene express large T antigen in thymocytes and in peripheral lymphoid cells, and develop tumors of both the thymus and the peripheral lymphoid organs. The ability of the human distal promoter to function appropriately in transgenic mice is consistent with the strong similarity observed between the mouse and human distal promoter sequences. With the exception of a single short interval that serves as a target for binding of nuclear factors, significant sequence similarity is not seen when the distal and proximal promoter sequences are compared. Hence, developmentally regulated, lineage-specific transcription of the lck gene is mediated by distinct promoter sequences that appear to be capable of functioning independently.

scholarly journals Genomic Promoter Analysis Predicts Functional Transcription Factor Binding

2008 ◽  
Vol 2008 ◽  
pp. 1-9 ◽  
Author(s):  
J. Sunil Rao ◽  
Suresh Karanam ◽  
Colleen D. McCabe ◽  
Carlos S. Moreno
Keyword(s):  
Transcription Factor ◽  
Binding Sites ◽  
Transcription Factor Binding Sites ◽  
Transcription Factor Binding ◽  
Proximal Promoter ◽  
Marginal Effect ◽  
Promoter Sequences ◽  
Factor Binding ◽  
Functional Transcription Factor

Background. The computational identification of functional transcription factor binding sites (TFBSs) remains a major challenge of computational biology. Results. We have analyzed the conserved promoter sequences for the complete set of human RefSeq genes using our conserved transcription factor binding site (CONFAC) software. CONFAC identified 16296 human-mouse ortholog gene pairs, and of those pairs, 9107 genes contained conserved TFBS in the 3 kb proximal promoter and first intron. To attempt to predict in vivo occupancy of transcription factor binding sites, we developed a novel marginal effect isolator algorithm that builds upon Bayesian methods for multigroup TFBS filtering and predicted the in vivo occupancy of two transcription factors with an overall accuracy of 84%. Conclusion. Our analyses show that integration of chromatin immunoprecipitation data with conserved TFBS analysis can be used to generate accurate predictions of functional TFBS. They also show that TFBS cooccurrence can be used to predict transcription factor binding to promoters in vivo.

Binding of Sp1 to the proximal promoter links constitutive expression of the human uPA gene and invasive potential of PC3 cells

Blood ◽  
2002 ◽  
Vol 100 (9) ◽  
pp. 3325-3332 ◽  
Author(s):  
Inés Ibañez-Tallon ◽  
Carmelo Ferrai ◽  
Elena Longobardi ◽  
Ileana Facetti ◽  
Francesco Blasi ◽  
...  
Keyword(s):  
Regulatory Region ◽  
Constitutive Expression ◽  
Proximal Promoter ◽  
Promoter Sequences ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Constitutive Gene Expression ◽  
Cellular Context ◽  
Pc3 Cells

Abstract Activated transcription of the urokinase-type plasminogen activator (uPA) gene depends on the enhancer, located approximately 2 kb from the start of transcription. The proximal promoter, driving basal transcription, contains a GC-/GA-rich sequence immediately upstream of the TATA box. We have investigated the role played by this element in the transcription of the uPA gene in HeLa and PC3 cells, which do not express or constitutively express the gene, respectively. This region binds either Sp1 or Sp3, as monomers or multimers, but not a combination of the 2 proteins. The more efficient binding of Sp1 to the proximal promoter in PC3 cells is correlated to its phosphorylation state. Polymerase chain reaction (PCR)–coupled, chromatin immunoprecipitation experiments with anti-Sp1 antibodies indeed show an enrichment of proximal promoter sequences in PC3 cells and support the observed difference in transcription levels from proximal promoter constructs in HeLa versus PC3 cells. Furthermore, overexpression of Sp1 increases transcription from the reporter construct in HeLa cells, whereas in PC3 cells, overexpression of Sp3 does not reduce transcription from the same construct, indicating that the Sp1/Sp3 balance cannot be shifted. We conclude that the GC-/GA-rich element of the uPA regulatory region is an independent functional element, regulated by Sp family proteins. Phosphorylation of Sp1 determines the presence in vivo and the functionality of this element in PC3 cells. Thus, the cellular context determines the relevance of the GC-/GA-rich region in uPA gene transcription, which contributes to constitutive gene expression, related, in turn, to the invasive phenotype.

scholarly journals Human chorionic somatomammotropin and growth hormone gene expression in rat pituitary tumour cells is dependent on proximal promoter sequences

1989 ◽  
Vol 17 (11) ◽  
pp. 4327-4337 ◽  
Author(s):  
Mark W. Nachtigal ◽  
Barbara E. Nickel ◽  
Margaret E. Klassen ◽  
Wengang Zhang ◽  
Norman L. Eberhardt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Pituitary Tumour ◽  
Tumour Cells ◽  
Proximal Promoter ◽  
Growth Hormone Gene ◽  
Promoter Sequences ◽  
Rat Pituitary ◽  
Chorionic Somatomammotropin
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